Blood samples were collected from the tail tip before euthanasia for glucose analysis and were assessed using a handheld glucometer (Accu-Chek Go Kit; Roche Diagnostic Brazil Ltd., São Paulo, Brazil). Fasting serum insulin concentrations were measured in blood samples collected after euthanasia and centrifuged at 1620× g for 10 min at 4 °C using an enzyme-linked immunosorbent assay (ELISA) kit (EMD Millipore Corporation, Billerica, MA, USA), according to manufacturer instructions.
Accu chek go kit
Manufactured by Roche
Sourced in Brazil
The Accu-Chek Go Kit is a portable blood glucose monitoring system designed for individuals with diabetes. It provides a convenient and reliable way to measure blood glucose levels. The kit includes a digital glucose meter, test strips, and a lancing device to obtain a small blood sample for analysis.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using accu chek go kit
1
Measuring Glucose and Insulin Levels
2
Plasma Lipid and Glucose Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma non-esterified fatty acids and triacylglycerol concentrations were measured using colorimetric assays (Wako Chemicals, Richmond, VA, USA; and Bioclin, Belo Horizonte, MG, Brazil, respectively). Blood glucose concentration was measured using the Accu-Chek Go Kit glucose analyzer (Roche Diagnostics Brazil Ltd., São Paulo, Brazil).
3
Glucose Tolerance and Insulin Resistance
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the end of treatment, the animals were fasted for 6 h. Blood samples from the tail tip were collected at baseline and after intraperitoneal administration of 30% glucose solution, equivalent to 2.0 g/kg body weight. Blood samples were collected at 0 min (baseline) and after 15, 30, 60, 90 and 120 min of glucose infusion, and analyzed using a handheld glucometer (Accu‐Chek Go Kit; Roche Diagnostic Brazil Ltda, Sao Paulo, Brazil). Glucose tolerance was assessed by the area under the curve (AUC). The homeostatic model assessment of insulin resistance (HOMA‐IR) was used as an insulin resistance index, calculated according to the formula: HOMA‐IR = [fasting glucose (mmol/L) × fasting insulin (μU/mL)]/22.5 (Matthews et al. 1985 ).
4
Glucose Tolerance Test in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 20 weeks, all rats were fasted for 6 h prior to the glucose tolerance test. After fasting, a blood sample from the tip of the tail was collected. The basal blood glucose level of each animal was immediately determined using a handheld glucometer (Accu-Chek Go Kit—Roche Diagnostic Brazil Ltda., São Paulo, Brazil). Subsequently, an injection of glucose solution (2 g/kg body weight) dissolved in water was administered intraperitoneally, and blood glucose levels were measured after 30, 60, 90, and 120 min [23 (link)]. Glucose intolerance was evaluated by the area under the curve (AUC) for glucose.
5
Glucose Tolerance Test in Animal Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucose tolerance test was performed after a 12-hour fast, and blood samples
collected from caudal artery were used for glucose analysis. Intraperitoneal
administration of 20% glucose (glucose monohydrate, Merck, São Paulo, Brazil)
(2 g/kg) was performed, followed by measurement of glucose levels at 30, 60, 90, 120,
180 and 240 minutes7 (link),10 , using a glucose meter (ACCU-CHEK GO
KIT, Roche Diagnostic Brazil Ltda, SP, Brazil).
Two days after in vivo analysis, the animals were kept under fasting
for 12 hours, anaesthetized intraperitoneally with sodium pentobarbital (50 mg/kg)
and euthanized by decapitation. Blood samples were collected, centrifuged (3,000 rpm)
and stored for further analysis. Biochemical analyses included measurements of serum
glucose, triglycerides, total cholesterol, HDL, LDL, albumin and total protein, using
an enzymatic method with specific kits.
collected from caudal artery were used for glucose analysis. Intraperitoneal
administration of 20% glucose (glucose monohydrate, Merck, São Paulo, Brazil)
(2 g/kg) was performed, followed by measurement of glucose levels at 30, 60, 90, 120,
180 and 240 minutes7 (link),10 , using a glucose meter (ACCU-CHEK GO
KIT, Roche Diagnostic Brazil Ltda, SP, Brazil).
Two days after in vivo analysis, the animals were kept under fasting
for 12 hours, anaesthetized intraperitoneally with sodium pentobarbital (50 mg/kg)
and euthanized by decapitation. Blood samples were collected, centrifuged (3,000 rpm)
and stored for further analysis. Biochemical analyses included measurements of serum
glucose, triglycerides, total cholesterol, HDL, LDL, albumin and total protein, using
an enzymatic method with specific kits.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!