Nitazoxanide

Manufactured by Selleck Chemicals

Sourced in United States

Nitazoxanide is a chemical compound used as a laboratory reagent. It is a white to pale yellow crystalline powder. The primary function of Nitazoxanide is to serve as a reference standard or analytical standard in chemical and pharmaceutical research and development.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (1 mentions) Superreal premix plus sybr green, by Tiangen Biotech (1 mentions) Mk 8776, by Selleck Chemicals (1 mentions) Proteinext mammalian total protein extraction kit, by Transgene (1 mentions) Beta actin monoclonal antibody, by Thermo Fisher Scientific (1 mentions) Fastking rt kit with gdnase, by Tiangen Biotech (1 mentions) Crystal violet, by Merck Group (1 mentions) Oligomycin, by Merck Group (1 mentions) Antimycin a, by Merck Group (1 mentions) Anisomycin, by Merck Group (1 mentions) Valinomycin, by Merck Group (1 mentions) Niclosamide, by Selleck Chemicals (1 mentions) Chloroquine, by MedChemExpress (1 mentions) Salinomycin, by MedChemExpress (1 mentions) Tilorone, by MedChemExpress (1 mentions) Homoharringtonine, by MedChemExpress (1 mentions) Atovaquone, by MedChemExpress (1 mentions) Gs 441524, by Biosynth (1 mentions) Vismodegib, by MedChemExpress (1 mentions) Gemcitabine, by MedChemExpress (1 mentions)

5 protocols using nitazoxanide

Nitazoxanide and Chk1 Inhibitors Protocol

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Nitazoxanide (NTZ) and the cell cycle checkpoint kinase 1 (Chk1) inhibitors MK-8776 and Prexasertib HCl were purchased from Selleck Chemicals (Selleck, Houston, TX, USA). The anti-VP2 monoclonal antibody was purchased from Ingenasa (Ingenasa, Madrid, Spain). Beta-actin monoclonal antibody was purchased from Thermo Fisher (Thermo Fisher, Waltham, MA, USA). The ProteinExt^® Mammalian Total Protein Extraction Kit was purchased from TransGen (TransGen Biotech, Beijing, China). Total RNA extraction was conducted using the RNeasy Mini Kit (QIAGEN, Hilden, Germany). The reverse transcription kit FastKing RT Kit (With gDNase) and quantitative PCR kit SuperReal PreMix Plus (SYBR Green) were purchased from TIANGEN (TIANGEN, Beijing, China).

Su X., Zhou H., Han Z., Xu F., Xiao B., Zhang J., Qi Q., Lin L., Zhang H., Li S, & Yang B. (2024). Transcriptional Differential Analysis of Nitazoxanide-Mediated Anticanine Parvovirus Effect in F81 Cells. Viruses, 16(2), 282.

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Comprehensive Chemical Compound Library

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Emetine (HY–B1479A, 99.81%, LCMS), salinomycin (HY-15597, >98%, NMR), tilorone (HY–B1080, 99.9%, LCMS), chloroquine (HY-17589, 99.9%, LCMS), homoharringtonine (HY-14944, 99.2%, LCMS), gemcitabine (HY–B0003, 99.9%, LCMS), vismodegib (HY-10440, 99.9%, LCMS), conivaptan (HY-18347A, 99.9%, LCMS), and atovaquone (HY-13832, 99.8%, LCMS) were purchased from MedChem Express (Monmouth Junction, NJ, USA); niclosamide (S3030, 99.8%, HPLC) and nitazoxanide (S1627, 99.3%, HPLC) were from Selleckchem (Houston, TX, USA); antimycin A (A8674, 97.64%, HPLC), anisomycin (A9789, >98%, HPLC) oligomycin (O4876, >90%, HPLC), valinomycin (V0627, ≧90%, HPLC) and crystal violet (C0775, Dye content ≥90%) were from Sigma–Aldrich (St. Louis, MO, USA); GS-441524 (AG167808, >98%, HPLC) were from Carbosynth (San Diego, CA, USA). All chemicals were used as supplied.

Yang C.W., Peng T.T., Hsu H.Y., Lee Y.Z., Wu S.H., Lin W.H., Ke Y.Y., Hsu T.A., Yeh T.K., Huang W.Z., Lin J.H., Sytwu H.K., Chen C.T, & Lee S.J. (2020). Repurposing old drugs as antiviral agents for coronaviruses. Biomedical Journal, 43(4), 368-374.

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Nitazoxanide and 5-FU Cytotoxicity Protocol

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Nitazoxanide was obtained from Selleck Chemicals. For comparison and reference, the standard cytotoxic drug 5-FU (Sigma-Aldrich; Merck KGaA) was included. 5-FU is the backbone therapy for colorectal cancer chemotherapy regimens and is also in clinical use as a radiosensitizer (1 (link),14 (link)).
The oxygen and moisture free MiniPod™ system (Roylan Developments Ltd.) was used to store source plates that were prepared with drugs solubilized in DMSO. The liquid handling system ECHO^® 550 (Labcyte, Inc.) was used to add the drugs to the experimental plates.

Karlsson H., Fryknäs M., Senkowski W., Larsson R, & Nygren P. (2022). Selective radiosensitization by nitazoxanide of quiescent clonogenic colon cancer tumour cells. Oncology Letters, 23(4), 123.

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Investigating NOX2 and ATM Inhibitors

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Nitazoxanide and Clotrimazole were obtained from Selleckchem and solubilized in DMSO (used as vehicle control in all experiments with these compounds). Ultrapure LPS for cell culture experiments (Lipopolysaccharide from Escherichia coli 0111:B4) was purchased from Invivogen. NOX2 inhibitor GSK2795039 was obtained from MedChem Express. ATM inhibitor KU-55933 was from Cell Signaling Technology.

Purandare N., Kunji Y., Xi Y., Romero R., Gomez-Lopez N., Fribley A., Grossman L.I, & Aras S. (2022). Lipopolysaccharide induces placental mitochondrial dysfunction in murine and human systems by reducing MNRR1 levels via a TLR4-independent pathway. iScience, 25(11), 105342.

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Cell Proliferation Assay and Western Blot Analysis

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HeLa, MGC803, A549 and 293 T cells were maintained in Dulbecco’s modified Eagles medium (Hyclone) supplemented with 10% (v/v) fetal bovine serum (Biological Industries) and transfected with polyethyleneimine (PEI) transfection reagent (Thermo Scientific). Cell proliferation assay was performed with 96-well plates, seeding about 1500 cells for each well. Every plasmid was transfected in 10–12 replicates. Sulforhodamine B (SRB) staining was performed 5 days after transfection. Western blot was performed with 6-well plates, 48 h after transfection. Antibodies against Tubulin (ProteinTech,1:5000), mCherry (ProteinTech, 1:1000), Bcl-2 (SAB, 1:1000), t-ERK (SAB, 1:1000), p-ERK (SAB, 1:1000), β-catenin (SAB, 1:1000), CyclinD1 (ProteinTech, 1:1000) and c-Myc (ProteinTech, 1:1000) were used. Stable cell lines were generated using lentiviral infection of HeLa cells in pLVX-IRES-Neo plasmids. Inhibitors against ERK (PD98059, Selleck), c-Myc (10058-F4, MCE), Cyclin D1 (Fascaplysin, Santa Cruz) and β-catenin (Nitazoxanide, Selleck) were purchased.

Zhou J., Tan Y., Zhang Y., Tong A., Shen X., Sun X., Jia D, & Sun Q. (2020). GEF-independent Ran activation shifts a fraction of the protein to the cytoplasm and promotes cell proliferation. Molecular Biomedicine, 1, 18.

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