12 13 epome

9,10-EpOME and 12,13-EpOME were purchased from Cayman chemical company (Ann Arbor, MI, USA). Eight sEH inhibitors used in a previous study^{11 (link)} included TPPU (‘C1’) trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea, PTUPB (‘C2’) 4-(5-phenyl-3-{3-[3-(4-trifluoromethyl-phenyl)-ureido]-propyl}-pyrazol-1-yl)-benzenesulfonamide, AUDA (‘C3’) 12-(3-adamantan-1-yl-ureido) dodecanoic acid, AEPU (‘C4’) 1-adamantanyl-3-(5-(2-(2-ethoxyethoxy)ethoxy)pentyl))urea, t-AUCB (‘C5’) trans-4-[4-(3-adamantan-1-yl-ureido)-cyclohexyloxy]-benzoic acid, t-TUCB (‘C6’) trans-4-{4-[3-(4-trifluoromethoxyphenyl)-ureido]-cyclohexyloxy}-benzoic acid, c-TUCB (‘C7’) cis-4-{4-[3-(4-trifluoromethoxyphenyl)-ureido]-cyclohexyloxy}-benzoic acid, and c-AUCB (‘C8’) cis-4-[4-(3-adamantan-1-yl-ureido)-cyclohexyloxy]-benzoic acid. Dexamethasone (DEX), [(11β, 16α)-9-fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-dione] and linoleic acid (LA) were purchased from Sigma-Aldrich Korea (Seoul, Korea).

Vasicine, vasicinone, isoorientin, isovitexin, vitexin, apigenin, 12,13-DiHOME, 9,10-DiHOME, 13(S)-HODE, 9(S)-HODE, 13-oxoODE, 9-oxoODE, 12,13-EpOME and 9,10-EpOME, 9-HpODE, and 7-ethoxyresorufin were purchased from Cayman Chemical (Ann Arbor, MI, USA). AITC, ionomycin calcium salt, HC-030039, and A967079 were from Sigma-Aldrich (St. Louis, MO, USA). DMSO was from Fisher Scientific (Waltham, MA, USA).

Whisker pads were removed from 7-week-old mice as described [25 (link)]. Fat covering the follicles was removed under a dissection microscope, before individual whiskers were plucked from the whisker pad. Immediately after isolation, the hair shaft was cut back to the follicle surface and transferred to a 48-well plate. Follicles were allowed to adhere for 1–2 min before culture medium was added. Samples were maintained in William’E medium (#22,551–089 Thermo Fisher Scientific GmbH, Dreieich, Germany) supplemented with hydrocortisone (10 ng/mL), insulin (10 µg/mL), 400 U/mL penicillin, 400 μg/mL, streptomycin, and glutamine (2 mmol/L). In some cases, samples were also treated with solvent (0.1% DMSO), FCS (5%), 12,13-EpOME (10 µmol/L, #52,450, Cayman Chemical, Tallinn, Estonia), 12,13-DiHOME (10 µmol/L, #10,009,832, Cayman Chemical, Tallinn, Estonia), or glycogen synthase kinase-3 inhibitor (10 µmol/L; # CHIR99021, Sigma-Aldrich, Darmstadt, Germany). Whisker follicles were imaged on days 0 and 3 and hair regrowth was analyzed from digital images using ImageJ software.