Cells were fixed in 4% paraformaldehyde (Sigma) and permeabilized with 0.1% Triton-X 100 (Sigma). Non-specific binding was blocked with 4% goat serum (Dako) in PBS for 1 h. Samples were immunostained with primary antibody against human cardiac sarcomeric α actinin raised in mouse (1:800; Sigma). After 24 h and a 0.1% Tween20 (Sigma) wash, samples were exposed to Cy3-conjugated goat anti-mouse secondary antibody IgG + IgM (1:250; Jackson Immuno Research) and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma). Samples were mounted in VectorShield mounting medium (Vector Labs, Peterborough, UK) and imaged using an automated fluorescence microscope (IMSTAR).
Primary antibody against human
Manufactured by Merck Group
This primary antibody is designed to detect human proteins. It is a key tool for researchers in the field of immunology, molecular biology, and cell biology. The antibody specifically binds to and recognizes human target proteins, enabling their identification and quantification in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Goat serum, by Agilent Technologies (2 mentions)
Cy3 conjugated goat anti mouse secondary antibody igg igm, by Jackson ImmunoResearch (2 mentions)
Paraformaldehyde (pfa), by Merck Group (2 mentions)
Triton x 100, by Merck Group (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
Vectorshield mounting medium, by Vector Laboratories (2 mentions)
2 protocols using primary antibody against human
1
Immunofluorescence Staining of Cardiac Cells
2
Immunofluorescence Staining of Cardiac Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were fixed in 4% paraformaldehyde (Sigma) and permeabilized with 0.1% Triton-X 100 (Sigma). Non-specific binding was blocked with 4% goat serum (Dako) in PBS for 1 h. Samples were immunostained with primary antibody against human cardiac sarcomeric α actinin raised in mouse (1:800; Sigma). After 24 h and a 0.1% Tween20 (Sigma) wash, samples were exposed to Cy3-conjugated goat anti-mouse secondary antibody IgG + IgM (1:250; Jackson Immuno Research) and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma). Samples were mounted in VectorShield mounting medium (Vector Labs, Peterborough, UK) and imaged using an automated fluorescence microscope (IMSTAR).
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