N tert butyl α phenylnitrone

Manufactured by Merck Group

Sourced in United States

N-tert-butyl-α-phenylnitrone is a chemical compound used as a lab equipment product. It functions as a spin trapping agent, which is used in electron spin resonance (ESR) spectroscopy to detect and identify short-lived free radicals.

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Lab products found in correlation

13 protocols using n tert butyl α phenylnitrone

Synthesis and Characterization of EPFR-Containing Particles

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The chemicals 4-Hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL, ≥97% nitrogen content, CAS 2226-96-2, Cat. No. 581500) and N-tert-butyl-α-phenylnitrone (PBN, ≥98%, CAS 3376-24-7) were purchased from Sigma Aldrich. Particles containing EPFRs were prepared as follows. The wetness impregnation method was applied to prepare Cab-o-sil/5% CuO particles [3 (link)]. EPFR-containing particles were acquired by dosing dichlorobenzene (DCB) vapor on the particles at 230 °C in vacuo. The samples were referred to as DCB-230.
Three controls were used in the experiments: Cab-o-sil particles dosed with DCB vapor at 50 °C in vacuo (Cab-o-sil-DCB); cab-o-sil/5% CuO particles; and water (vehicle control).

Guan X., Truong L., M. Lomnicki S., L. Tanguay R, & A. Cormier S. (2021). Developmental Hazard of Environmentally Persistent Free Radicals and Protective Effect of TEMPOL in Zebrafish Model. Toxics, 9(1), 12.

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Pharmacological Agents for Cell Studies

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Adenosine 5′-triphosphate disodium salt hydrate (ATP), N-acetylcysteine (NAC), apocynin and N-tert-butyl-α-phenylnitrone (PBN) were purchased from Sigma Aldrich (St. Louis, MO). CNQX, D-AP5, 2′(3′)-O-(4-Benzoylbenzoyl)adenosine-5′-triphosphate tri(triethylammonium) salt (BzATP) and A438079 were purchased from Tocris (Bristol, UK). All compounds were dissolved in Milli-Q water or dimethyl sulfoxide (DMSO) as stock solutions and further diluted to final concentrations in 0.1% DMSO.

Munoz F.M., Gao R., Tian Y., Henstenburg B.A., Barrett J.E, & Hu H. (2017). Neuronal P2X7 receptor-induced reactive oxygen species production contributes to nociceptive behavior in mice. Scientific Reports, 7, 3539.

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Oxidative Stress and Calcium Signaling

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L-OHP and allyl isothiocyanate (AITC) were purchased from Wako Pure Chemical Industries (Osaka, Japan). N-tert-butyl-α-phenylnitrone (PBN), cremophore EL, 2-aminoethoxydiphenyl borate (2-APB), poly-L-lysine, and D-mannitol were purchased from Sigma-Aldrich (St. Louis, MO). The 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) was acquired from Dojindo Laboratories (Kumamoto, Japan). The mitoTEMPO was obtained from Santa Cruz (Dallas, TX). Peroxy Green 1 (PG-1) was synthesized previously (Miyake et al., 2016 (link)) according to the literature (Miller et al., 2007 (link)). Other drugs and chemicals were obtained from Nacalai Tesuque (Kyoto, Japan).

Miyake T., Nakamura S., Meng Z., Hamano S., Inoue K., Numata T., Takahashi N., Nagayasu K., Shirakawa H., Mori Y., Nakagawa T, & Kaneko S. (2017). Distinct Mechanism of Cysteine Oxidation-Dependent Activation and Cold Sensitization of Human Transient Receptor Potential Ankyrin 1 Channel by High and Low Oxaliplatin. Frontiers in Physiology, 8, 878.

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Quantification of Zearalenone by EPR

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Pure zearalenone standards in acetonitrile (50 mg/L) were purchased from ANPEL (ANPEL Laboratory Technologies Co., Ltd., Shanghai, China). Zearalenone were diluted in acetonrtrile to obtain a concentration of 50 ng/mL and stored at −20 °C. 5,5-dimethyl-1-pyrroline-N-oxide (DMPO); 2,2,6,6-tetramethylpiperidine (TEMP, >98.0%) and N-tert-Butyl-α-phenylnitrone (PBN) were purchased from Sigma (Sigma Aldrich Co., Ltd., Shanghai, China). All of other reagents were provided by Aladdin (Aladdin Chemicals Co., Ltd., Shanghai, China).

Zheng Z., Huang Y., Liu L., Chen Y., Wang Y, & Li C. (2022). Zearalenone Degradation by Dielectric Barrier Discharge Cold Plasma: The Kinetics and Mechanism. Foods, 11(10), 1494.

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ROS Scavengers Modulate Cancer-Induced Bone Pain

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N-tert-Butyl-α-phenylnitrone (PBN) and 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (Tempol) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in 0.9% saline. The dose of drugs was chosen according to our preliminary results and previous reports [17] (link), [18] (link). To determine whether ROS scavengers could alleviate CIBP in advanced phase, a single dose of PBN (10, 50, 100 mg/kg, i.p.) or Tempol (10, 100, 200 mg/kg, i.p.) was given on day 14 (14 days) after the establishment of CIBP models. To determine whether repetitive treatment with ROS scavengers had a cumulative analgesic effect on CIBP, PBN (100 mg/kg, i.p.) or Tempol (200 mg/kg, i.p.) was given once daily from 14 days to 18 days. To determine whether early treatment with ROS scavengers could suppress the development of CIBP, PBN (100 mg/kg, i.p.) or Tempol (200 mg/kg, i.p.) was given once daily from 1 days to 5 days. To determine whether ROS scavengers could affect the mechanical sensitivity of naive rats, PBN (10, 50, 100 mg/kg, i.p.) or Tempol (10, 100, 200 mg/kg, i.p.) was given once daily for 5 consecutive days.

Zhou Y.Q., Liu D.Q., Chen S.P., Sun J., Zhou X.R., Rittner H., Mei W., Tian Y.K., Zhang H.X., Chen F, & Ye D.W. (2017). Reactive oxygen species scavengers ameliorate mechanical allodynia in a rat model of cancer-induced bone pain. Redox Biology, 14, 391-397.

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Synthesis and Characterization of PCL Polymer

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PCL pellets (M_n = 80,000 g/mol), chloroform (CHCl₃, > 99%), N,N-dimethylformamide (DMF, > 99%) N-tert-butyl-α-phenylnitrone (PBN, 98%), sodium carbonate (Na₂CO₃), sodium bicarbonate (NaHCO₃), sodium nitrate (NaNO₃), potassium chloride (KCl), and formic acid (HCOOH) were purchased from Sigma-Aldrich and used without further purification. Argon gas (Alphagaz 1) was purchased from Air Liquide. Ultrapure grade water (milliQ water) was obtained by filtration of deionized water with a Millipore system.

Grande S., Tampieri F., Nikiforov A., Giardina A., Barbon A., Cools P., Morent R., Paradisi C., Marotta E, & De Geyter N. (2019). Radicals and Ions Formed in Plasma-Treated Organic Solvents: A Mechanistic Investigation to Rationalize the Enhancement of Electrospinnability of Polycaprolactone. Frontiers in Chemistry, 7, 344.

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Photoactive Siloxane-Based Polymer Synthesis

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The (3-Glycidyloxypropyl)trimethoxysilane (GPTMS), 3-(Trimethoxysilyl)propyl methacrylate (MAPTMS), dipentene dioxide (DPDO), (3,4-epoxycyclohexane)methyl 3,4-epoxycyclohexylcarboxylate (EPOX), thioxanthone (TX), soybean oil acrylate (SOA), and bis(4-methylphenyl)iodonium hexafluorophosphate (98%) were purchased from Sigma-Aldrich. The 5,5-dimethyl-1-pyrroline N-oxide (DMPO, distilled prior to the application), N-tert-butyl-α-phenylnitrone (PBN), and nitrosodurene (ND) were provided by Sigma-Aldrich, and the 2,2-dimethyl-4-phenyl-2H-imidazole 1-oxide (DMPIO) was purchased from Enzo Life Sciences. All the solvents were purchased from Sigma Aldrich (Saint-Quentin-Fallavier, France) and used without any further purification. Table 2 summarizes the structures of the compounds used in the investigation.

Nguyen T.T., Breloy L., Rios De Anda A., Hayek H., Chiappone A., Malval J.P., Grande D, & Versace D.L. (2024). Thioxanthone-Based Siloxane Photosensitizer for Cationic/Radical Photopolymerization and Photoinduced Sol–Gel Reactions. Molecules, 29(1), 255.

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Emulsion Formulation and Characterization

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Glucose syrup (DE38, C*Dry 1934) was provided by Cargill Germany GmbH (Krefeld, Germany). Dextran (Molecular weight 70 kDa) was kindly provided by Pharmacosmos A/S (Holbaek, Denmark). Pullulan (molecular weight 200 kDa) was provided by Hayashibara Co., Ltd. (Okayama, Japan). Whey Powder Concentrate (WPC) was provided by ARLA Food Ingredients (Viby, Denmark). MCT oil, under the commercial name MIGLYOL, was kindly provided by IOI Oleo GmbH (Witten, Germany). Fish oil with a content of 9.3 wt.% EPA and 10.9 wt.% DHA was delivered by Maritex A/S (Sortland, Norway). The peroxide value (PV) of the fish oil was 0.4±0.1 meq/kg oil. Citrem (esters of citric acid without antioxidants), with a PV of 2.3±0.1 meq/kg oil, was provided by Danisco (Copenhagen, Denmark). ESR probes 16-DOXYL-stearic acid (DSA) and N-tert-Butyl-α-phenylnitrone (PBN) were purchased from Sigma Aldrich (Søborg, Denmark). Oxygen (≥ 99.5%) and nitrogen (≥ 99.999%) gasses were supplied by Air Liquide (Taastrup, Denmark)

Boerekamp D.M.W., Andersen M.L., Jacobsen C., Chronakis I.S, & García-Moreno P.J. (2019). Oxygen permeability and oxidative stability of fish oil-loaded electrosprayed capsules measured by Electron Spin Resonance: Effect of dextran and glucose syrup as main encapsulating materials. Food chemistry, 287.

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Optimized Hop Resin Extract Protocol

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Sodium acetate trihydrate was purchased from VWR International (Radnor, PA, USA) and 200 proof ethanol from Decon Labs, Inc. (King of Prussia, PA, USA). Pilsen dry malt extract (DME) was purchased from Briess Malt & Ingredients (Chilton, WI, USA), Cascade HopShot hop resin from Northern Brewer (Roseville, MN, USA), and 1056 American Ale yeast from Wyeast (Hood River, OR, USA). Sodium azide was obtained from Mallinckrodt Chemicals (Dublin, Ireland). Nitric acid was purchased from J.T. Baker Chemical Co. (Phillipsburg, NJ, USA) and was twice-distilled in two Savillex DST-1000 acid purification systems (Savillex, Eden Prairie, MN, USA) prior to use. N-tert-Butyl-α-phenylnitrone (PBN) and 4-mercapto-4-methylpentan-2-one (4-MMP) were purchased from MilliporeSigma (Burlington, MA, USA). Hydrogen peroxide for trace metal analysis was obtained from VWR International (Radnor, PA, USA). Ultra-high purity argon (99.999%), nitrogen (99.995%), and carbon dioxide (99.5%) were supplied by Penn State General Stores (University Park, PA, USA). All hops were grown at Penn State's Russell E. Larson Research Farm (Pennsylvania Furnace, PA, USA) in 2018 and 2019.

Chrisfield B.J., Hopfer H., & Elias R.J. (2020). Impact of Copper Fungicide Use in Hop Production on the Total Metal Content and Stability of Wort and Dry-Hopped Beer. Beverages, 6(3), 48-48.

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Protein Extraction and Quantification

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Agarose, diluted trypsin (0.25%),
RIPA buffer, N-tert-butyl-α-phenylnitrone
(PBN), N,N′-dimethylthiourea
(DMTU), and 1-palmitoyl-sn-glycero-3-phosphocholine
(LysoPC(16:0)) were purchased from Millipore Sigma (St. Louis MO).
Hydrogen peroxide, Pierce’s gold standard BCA assay, trypsin,
tris base, hydrochloric acid, acetone, formic acid, Pierce colorometric
peptide quantification assay, MS grade 0.1% formic acid in water,
MS grade 0.1% formic acid in acetonitrile, and MS grade water were
purchased from Thermo Fisher Scientific (Waltham, MA). McCoy’s
5A, fetal bovine serum (FBS), l-glutamine, phosphate-buffered
saline (PBS), and 0.05% porcine trypsin with 0.1% EDTA were purchased
from Gibco (Gaithersburg, MD). Iodoacetamide (IAA) was acquired from
ACROS Organics. Dithiothreitol (DTT) was purchased from American Bio
(Canton, MA). Dimethyl sulfoxide (DMSO) was purchased from Invitrogen
(Carlsbad, CA).

Shortt R.L., Wang Y., Hummon A.B, & Jones L.M. (2023). Development of Spheroid-FPOP: An In-Cell Protein Footprinting Method for 3D Tumor Spheroids. Journal of the American Society for Mass Spectrometry, 34(3), 417-425.

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