Cd161 pe

Manufactured by BioLegend

CD161-PE is a fluorescent-labeled antibody that binds to the CD161 receptor expressed on natural killer cells and a subset of T cells. It is used for the identification and analysis of these cell populations in flow cytometry applications.

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Lab products found in correlation

Mouse tumor dissociation kit, by Miltenyi Biotec (1 mentions) Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Cd11b fitc, by BD (1 mentions) Cd62l bv785, by BioLegend (1 mentions) F4 80 af647, by BioLegend (1 mentions) Cd4 bv510, by BioLegend (1 mentions) Cd11c pe, by BioLegend (1 mentions) Cd44 apc cy7, by BD (1 mentions) 70 m filter, by BD (1 mentions) Anti cd16 32, by BD (1 mentions) Cd45 bv605, by BD (1 mentions) Cd3 buv737, by BD (1 mentions) Cd8a bb515, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Ifn γ bv510, by BioLegend (1 mentions) Aqua live dead amine dye amcyan, by Thermo Fisher Scientific (1 mentions) Cd4 bv605, by BD (1 mentions) Tnf α alexafluor 700, by BD (1 mentions) Cd69 pe cf594, by BD (1 mentions) Cd8 bv650, by BD (1 mentions)

Close spelling variants of this product

Anti‐CD161‐PE Anti-CD161 PE-Cy7 (HP-3G10, Anti-CD161-PE/Dazzle-594 Anti-CD161 PE-Cy7

2 protocols using cd161 pe

Tumor Immune Cell Profiling Protocol

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Tumors were minced and digested by using the mouse Tumor Dissociation Kit (Miltenyi Biotech), washed with FACS buffer (PBS containing 2% FBS, 2 mM EDTA and 0.02% sodium azide), and filtered through a 70 -µm filter (BD Biosciences). The obtained single-cell suspension was stained with LIVE/DEAD Fixable Blue Dead Cell Stain kit (Thermo Fisher Scientific), blocked with anti-CD16/32 (BD Biosciences; #553142; clone 2.4G2; 1:1000), stained with fluorochrome-labeled antibodies, and analyzed using a LSR Fortessa (BD Biosciences) and FlowJo software (Tree Star Inc).
The following antibodies were purchased from BD Biosciences: CD3-BUV737 (#564380; clone 17A2; 1:400), CD8a-BB515 (#564422; clone 53-6.7; 1:400), CD44-APC-Cy7 (#560568; clone IM7; 1:1000), CD274-BV711 (#563369; clone MIH5; 1:200), CD11b-FITC (#557672; clone M1/70; 1:800), CD45-BV605 (#563053; clone 30F11; 1:400). The following antibodies were purchased from Biolegend: CD4-BV510 (#100449; clone GK1.5; 1:400), CD62L-BV785 (#104440; clone MEL-14; 1:200), CD161-PE (#108707; clone PK136; 1:400), CD11c-PE (#117308; clone N418; 1:200), F4/80-AF647 (#123122; clone BM8; 1:2000).

Galvani E., Mundra P.A., Valpione S., Garcia-Martinez P., Smith M., Greenall J., Thakur R., Helmink B., Andrews M.C., Boon L., Chester C., Gremel G., Hogan K., Mandal A., Zeng K., Banyard A., Ashton G., Cook M., Lorigan P., Wargo J.A., Dhomen N, & Marais R. (2020). Stroma remodeling and reduced cell division define durable response to PD-1 blockade in melanoma. Nature Communications, 11, 853.

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Multicolor Flow Cytometry for Immune Profiling

Cited 1 time

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Multi-color flowcytometric analysis was performed on cells according to standard procedures using anti-human mAbs that cross-react with rhesus macaques. The following antibodies were used at predetermined optimal concentrations: CD45 (BD clone D058-1283), CD3 APC-Cy7 (BD clone SP34-2), CD4 BV605 (BD clone L200), CD8 BV650 (BD clone SK1), CD69 PE-CF594 (BD clone FN50), CD161 PE (Biolegend clone HP- 3G10), TCR γδ PE-Cy7 (BD clone B1), TCR Vδ1 FITC (ThermoScientific clone TS8.2), TCR Vδ2 FITC (ThermoScientific clone 15D), TCR Vα7.2 BV421 (Biolegend clone 3C10), and Aqua Live/Dead amine dye-AmCyan from Invitrogen (Waltham, MA). Surface staining was carried out by standard procedures as earlier described (23 (link)). Flow cytometric acquisition was performed on the BD Fortessa instrument driven by the FACS DiVa software for at least 100,000 CD3+ T cells in PBMC or at least 10,000 CD3+ T cells for rectal biopsy lymphocytes. The data acquired were analyzed using FlowJo software (version 10.7.1; TreeStar, Ashland, OR). For evaluation of cytokine production, intracellular cytokine staining with IFN-γ BV510 (Biolegend clone 4S.B3), IL-17 PerCP-Cy5.5 (eBioscience clone eBio64DEC17), IL-22 APC (Invitrogen clone IL22JOP), and TNF-α Alexa Fluor 700 (BD clone MAb11) were utilized.

Walker E.M., Slisarenko N., Gerrets G.L., Grasperge B.F., Mattison J.A., Kissinger P.J., Welsh D.A., Veazey R.S., Jazwinski S.M, & Rout N. (2021). Dysregulation of IL-17/IL-22 Effector Functions in Blood and Gut Mucosal Gamma Delta T Cells Correlates With Increase in Circulating Leaky Gut and Inflammatory Markers During cART-Treated Chronic SIV Infection in Macaques. Frontiers in Immunology, 12, 647398.

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