Epl 405

Manufactured by Edinburgh Instruments

Sourced in Japan

The EPL-405 is a pulsed diode laser from Edinburgh Instruments. It operates at a wavelength of 405 nm and provides pulse widths of 60 ps to 2 ns. The laser is designed for time-resolved fluorescence and phosphorescence applications.

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Lab products found in correlation

Fls980 spectrophotometer, by Edinburgh Instruments (2 mentions) Triax190, by Horiba (2 mentions) Microhr, by Horiba (2 mentions) Gemini 180, by Horiba (2 mentions) Ep led 340, by Edinburgh Instruments (2 mentions) Fls920, by Edinburgh Instruments (2 mentions) Symphony 2, by Horiba (1 mentions) Ray tube, by Philips (1 mentions) Triax 180, by Horiba (1 mentions) Cary eclipse spectrophotometer, by Agilent Technologies (1 mentions) Cary 50 uv vis spectrophotometer, by Agilent Technologies (1 mentions) Fls1000 spectrometer, by Edinburgh Instruments (1 mentions)

10 protocols using epl 405

Time-resolved Photoluminescence Decay Analysis

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Time-dependent PL intensity
decays were measured after the PLQY measurements on the same samples
using the time-correlated single-photon counting technique using a
FLS920 Edinburgh Instruments spectrofluorimeter. The PL intensity
decay curves are shown on a logarithmic scale. The samples were excited
with a 401.2 nm picosecond pulsed diode laser (EPL-405, Edinburgh
Instruments) and a 500 ns pulse period. The PL decays were collected
at the peak of the emission spectra with an emission slit width of
3–5 nm.

Akkerman Q.A., Bladt E., Petralanda U., Dang Z., Sartori E., Baranov D., Abdelhady A.L., Infante I., Bals S, & Manna L. (2019). Fully Inorganic Ruddlesden–Popper Double Cl–I and Triple Cl–Br–I Lead Halide Perovskite Nanocrystals. Chemistry of Materials, 31(6), 2182-2190.

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Steady-state RL Measurements via CCD

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Steady state RL measurements were carried out at room temperature using a homemade apparatus featuring, as a detection system, a liquid nitrogen-cooled, back-illuminated, and UV-enhanced charge coupled device (CCD) Jobin-Yvon Symphony II, combined with a monochromator Jobin-Yvon Triax 180 equipped with a 100 lines/mm grating. All spectra are corrected for the spectral response of the detection system. RL excitation was obtained by unfiltered X-ray irradiation through a Be window, using a Philips 2274 X-ray tube with tungsten target operated at 20 kV. At this operating voltage, a continuous X-ray spectrum is produced by a Bremsstrahlung mechanism superimposed to the L and M transition lines of tungsten, due to the impact of electrons generated through thermionic effect and accelerated onto a tungsten target. The dose rate was 0.2 Gy/s, evaluated by comparison with a calibrated ⁹⁰Sr-⁹⁰Y beta radioactive source and using optically stimulated luminescence emission from quartz crystalline powder (100 – 200 μm grains). In order to record the PL measurements, the same acquisition system of RL measurements has been coupled to a 405 nm pulsed diode laser (EPL-405 Edinburgh Instruments) through a quartz optical fiber bundle allowing the illumination of the sample in the X-ray chamber.

Perego J., Bezuidenhout C.X., Villa I., Cova F., Crapanzano R., Frank I., Pagano F., Kratochwill N., Auffray E., Bracco S., Vedda A., Dujardin C., Sozzani P.E., Meinardi F., Comotti A, & Monguzzi A. (2022). Highly luminescent scintillating hetero-ligand MOF nanocrystals with engineered Stokes shift for photonic applications. Nature Communications, 13, 3504.

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Absorption and Photoluminescence Characterization

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Absorption spectra
were recorded using a Cary Lambda 900 spectrophotometer at normal
incidence with Suprasil quartz cuvettes with a 0.1 cm optical path
length. Steady-state PL and PL excitation spectra have been recorded
using a xenon lamp as an excitation source, together with a double
monochromator (Jobin-Yvon Gemini 180 with a 1200 grooves/mm grating),
and recorded through a nitrogen-cooled charge-coupled device (CCD)
detector coupled to a monochromator (Jobin-Yvon Micro HR). Under cw
laser excitation, signals have been recorded using a nitrogen-cooled
CCD coupled with a double monochromator, Triax- 190 (HORIBA Jobin-Yvon),
with a spectral resolution of 0.5 nm. All spectra have been corrected
for the setup optical response. Time-resolved PL spectra have been
recorded using a pulsed light-emitting diode (LED) at 250 nm (3.65
eV, EP-LED 340 Edinburgh Instruments, a pulse width of 700 ps) or
a pulsed laser at 405 nm (3.06 eV, EPL-405 Edinburgh Instruments,
a pulse width of 150 ps) as a light source. Data were obtained with
an Edinburgh Instruments FLS-980 spectrophotometer, with a 5 nm bandwidth
and a time resolution of 0.1 ns.

Secchi V., Cova F., Villa I., Babin V., Nikl M., Campione M, & Monguzzi A. (2023). Energy Partitioning in Multicomponent Nanoscintillators for Enhanced Localized Radiotherapy. ACS Applied Materials & Interfaces, 15(20), 24693-24700.

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Steady-State and Time-Resolved Optical Spectroscopy

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Steady-state absorption spectra were recorded with a Cary 50 UV-VIS spectrophotometer (Varian Inc., Palo Alto, CA, USA). PL measurements were carried out with a Cary Eclipse spectrophotometer (Varian Inc., Palo Alto, CA, USA). Fluorescence decay measurements were performed with a F920 spectrophotometer (Edinburgh Instruments, Livingston, UK), equipped with a single photon photomultiplier detector (S900-R) (Hamamatsu, Shizuoka, Japan) and a picosecond pulsed diode laser (EPL-405) (excitation wavelength 405 nm, pulse width 66.9 ps, repetition rate 2 MHz) (Edinburgh Instruments, Livingston, UK). Quartz cuvettes with the optical path length of 1 cm were used for all measurements.

Skripka A., Dapkute D., Valanciunaite J., Karabanovas V, & Rotomskis R. (2018). Impact of Quantum Dot Surface on Complex Formation with Chlorin e6 and Photodynamic Therapy. Nanomaterials, 9(1), 9.

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Fluorescence Lifetime Spectroscopy Technique

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An Edinburgh Instruments FLS920 combined fluorescence
lifetime and steady-state spectrometer was used for all emission measurements.
Samples were held in a l × 1 cm quartz fluorescence cuvette and
either degassed using three freeze–pump–thaw cycles
on a Schlenk line or prepared in a glovebox. Excited-state lifetime
measurements were performed using the time-correlated photon counting
method, with a pulsed diode laser (EPL-405, Edinburgh Instruments)
as an excitation source.

Summers P.A., Calladine J.A., Ghiotto F., Dawson J., Sun X.Z., Hamilton M.L., Towrie M., Davies E.S., McMaster J., George M.W, & Schröder M. (2015). Synthesis and Photophysical Study of a [NiFe] Hydrogenase Biomimetic Compound Covalently Linked to a Re-diimine Photosensitizer. Inorganic Chemistry, 55(2), 527-536.

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Time-Correlated Single-Photon Counting

Cited 1 time

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TCSPC
has been previously described in detail.^{17 (link)} Briefly, the excitation of the sample was done with a picosecond
diode laser (Edinburgh Instruments, EPL405) at 404.6 nm (instrument
response function (IRF) ≈ 77.1 ps pulses). SpectraSolve was
used for the TCSPC data analysis, as described before.^{17 (link)}

El-Zohry A.M., Agrawal S., De Angelis F., Pastore M, & Zietz B. (2020). Critical Role of Protons for Emission Quenching of Indoline Dyes in Solution and on Semiconductor Surfaces. The Journal of Physical Chemistry. C, Nanomaterials and Interfaces, 124(39), 21346-21356.

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Fluorescence Lifetime Analysis of Nanoparticles

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Fluorescence lifetimes were taken on an Edinburgh Instruments FLS1000 spectrometer at sample concentrations of 0.5 μM to 1 μM in deionized water and in a 3 mL 10 mm pathlength quartz cuvette at 90 degrees. Particle samples were excited using a 405 nm pulsed excitation (Edinburgh Instruments EPL-405 pulsed diode laser with ~500 ps pulsewidth, 5 mW) operated at a 500 ns pulse period. Fluorescence was collected with a photon counting photomultiplier tube and decay curves were fitted in the Edinburgh Instruments software using

I (t) = \int_{- \infty}^{t} I R F (t^{'}) \sum_{i = 1}^{n} α_{i} exp (\frac{- t - t'}{τ_{i}}) d t

where I(t) is the fluorescence decay function, IRF(t′) the instrument response function, α_i is the amplitude of the i-th lifetime, τ_i, and n is the number of fitting components. The IRF was determined using 40 nm SNPs. The fit quality was evaluated based on χ² values. All decay curves were fitted to a bi-exponential decay model (n=2).

Kohle F.F., Hinckley J.A, & Wiesner U.B. (2019). Dye Encapsulation in Fluorescent Core-Shell Silica Nanoparticles as Probed by Fluorescence Correlation Spectroscopy. The journal of physical chemistry. C, Nanomaterials and interfaces, 123(15), 9813-9823.

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Time-Resolved Photoluminescence Spectroscopy

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Time-dependent PL intensity decays were measured after the PLQY
measurements on the same samples using the time-correlated single-photon
counting technique using a FLS920 Edinburgh Instruments spectrofluorimeter. The
PL intensity decay curves are shown on a logarithmic scale. The samples were
excited with a 401.2 nm picosecond pulsed diode laser (EPL-405, Edinburgh
Instruments) and a 500 ns pulse period. The PL decays were collected at the peak
of the emission spectra with an emission slit width of 3-5 nm.

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Time-Correlated Single Photon Counting Setup

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The TCSPC setup
has been described in detail previously.^{21 (link)} Briefly, the sample was excited with a picosecond diode laser (Edinburgh
Instruments, EPL405) at 404.6 nm (77.1 ps pulses), giving an instrument
response function of ca. 100 ps.

El-Zohry A.M., Orabi E.A., Karlsson M, & Zietz B. (2021). Twisted Intramolecular Charge Transfer (TICT) Controlled by Dimerization: An Overlooked Piece of the TICT Puzzle. The Journal of Physical Chemistry. a, 125(14), 2885-2894.

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Steady-State and Time-Resolved Spectroscopy of NTs

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Steady-state PL and PLE spectra have
been recorded using a xenon lamp as an excitation source, together
with a double monochromator (Jobin-Yvon Gemini 180 with a 1200 grooves/mm
grating), and recorded through a nitrogen-cooled CCD detector coupled
to a monochromator (Jobin-Yvon Micro HR). Under cw laser excitation, signals have been recorded using a nitrogen-cooled
CCD coupled with a double monochromator, Triax-190 (HORIBA Jobin-Yvon),
with a spectral resolution of 0.5 nm. The PL quantum yield of bare
NTs has been measured with relative methods using 2,5-diphenyloxazole
as a fluorescence standard.⁶⁶ All spectra
have been corrected for the setup optical response. Time-resolved
PL spectra have been recorded using a pulsed LED at 340 nm (3.65 eV,
EP-LED 340 Edinburgh Instruments, a pulse width of 700 ps) or a pulsed
laser at 405 nm (3.06 eV, EPL-405 Edinburgh Instruments, a pulse width
of 150 ps) as a light source. Data were obtained with an Edinburgh
Instruments FLS-980 spectrophotometer, with a 5 nm bandwidth and a
time resolution of 0.1 ns.

Villa I., Villa C., Crapanzano R., Secchi V., Tawfilas M., Trombetta E., Porretti L., Brambilla A., Campione M., Torrente Y., Vedda A, & Monguzzi A. (2021). Functionalized Scintillating Nanotubes for Simultaneous Radio- and Photodynamic Therapy of Cancer. ACS Applied Materials & Interfaces, 13(11), 12997-13008.

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