Anti ki67 antibodies

Manufactured by Merck Group

Sourced in United States

Anti-Ki67 antibodies are laboratory reagents used to detect the Ki67 protein, which is a marker of cellular proliferation. These antibodies are commonly used in immunohistochemistry and other research applications to assess the proliferative activity of cells.

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Anti-Ki67 (31 citations)

2 protocols using anti ki67 antibodies

TRAIP Antibody Production and Validation

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The TRAIP polyclonal antibody was raised against GST-TRAIP-N terminal fusion protein (see Figure 3e) and affinity purified using column coated with MBP-TRAIP-N terminal fusion protein. Antibody specifically recognizing γH2AX was previously described [42 (link)]. The anti-PCNA (PC10) and anti-CHK1 (G-4) antibodies were from Santa Cruz (Dallas, TX, USA); anti-Ki67 antibodies were from Chemicon (Darmstadt, Germany); anti-RPA1 antibodies were from Calbiochem (Darmstadt, Germany); anti-Chk1-pS345 antibodies were from Cell Signaling (Danvers, MA, USA); anti-KAP1 antibodies were from BD Transduction Laboratories (San Jose, CA, USA). Anti-Actin, anti-GFP and anti-Flag (M2) antibodies were obtained from Sigma (Darmstadt, Germany). ATMi (KU55993) and ATRi (VE821) inhibitors were from SelleckChem (Houston, TX, USA).

Feng W., Guo Y., Huang J., Deng Y., Zang J, & Huen M.S. (2016). TRAIP regulates replication fork recovery and progression via PCNA. Cell Discovery, 2, 16016-.

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Antibodies for TRAIP, γH2AX, and DNA damage

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The anti-TRAIP polyclonal antibodies were raised against GST tagged TRAIP-N terminal fusion proteins and were affinity-purified using column coated with N-terminal MBP tagged TRAIP as previously described (29 (link)). Anti-γH2AX antibodies were previously described (30 (link)). The anti-S9.6 antibodies were from Kerafast (Boston, USA); anti-Nucleolin (C23) antibodies were from Santa Cruz (Dallas, TX, USA); anti-Ki67 antibodies were from Chemicon (Darmstadt, Germany). (Z)-4-Hydroxytamoxifen (4-OHT), anti-Actin, anti-Flag (Rabbit) and anti-Flag (Mouse; Clone M2) antibodies were from Sigma (Darmstadt, Germany); anti-pRPA32 antibodies were from Bethyl Laboratories. Shield-1 was purchased from Takara (Kanagawa, Japan). Hydroxyurea, Aphidicolin, Mitomycin C, Cisplatin, Actinomycin D, α-Amanitin, and DRB were from Sigma (Darmstadt, Germany). CX5461, ATM inhibitor (ATMi; KU55933) and ATR inhibitor (ATRi; VE821) were from SelleckChem (Houston, TX, USA). DAPI (4′,6-diamidino-2-phenylindole) and DNase I (EN0523) was from Thermo Fisher Scientific. RNase A was from Qiagen.

Chen Y., Li J., Cao F., Lam J., Cheng C.C., Yu C.H, & Huen M.S. (2018). Nucleolar residence of the seckel syndrome protein TRAIP is coupled to ribosomal DNA transcription. Nucleic Acids Research, 46(19), 10119-10131.

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