MMP is key toward maintaining mitochondrial function, which was measured with a commercial kit of JC-1 (Beyotime). The procedure was performed according to the manufacturer’s instructions. Mitochondria from liver tissue were separated using a tissue mitochondria extraction kit (Beyotime) and were then incubated with JC-1 solution for 30 min at 37°C. The fluorescence in different groups was measured with a micro-plate reader (Bio-Rad, Hercules, CA, USA). The MMP percentage was finally calculated in order to evaluate the loss of MMP in the liver.
Tissue mitochondria extraction kit
Manufactured by Beyotime
Sourced in China
The Tissue Mitochondria Extraction Kit is a laboratory tool designed for the isolation and purification of mitochondria from various tissue samples. The kit provides a standardized protocol and reagents to facilitate the extraction process, enabling researchers to obtain high-quality mitochondrial fractions for further analysis and experimentation.
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Lab products found in correlation
2 protocols using tissue mitochondria extraction kit
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Mitochondrial Membrane Potential Assay
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Mitochondrial Membrane Potential Measurement
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The mitochondrial membrane potential (ΔΨm) was determined using a JC-1 probe kit (Beyotime, Shanghai, China). Firstly, a small section (50 mg) of liver was excised and weighted immediately after rat sacrifice. Liver mitochondria was obtained by a tissue mitochondria extraction kit (Beyotime, Shanghai, China). Thereafter, the purified mitochondria were stained with a 1× JC-1 working solution, and samples were detected by a fluorescence microplate. ΔΨm was calculated as the ratio of red fluorescence (JC-1 aggregates) to green fluorescence (JC-1 monomers).
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