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Anti ero1 lα

Manufactured by Cell Signaling Technology
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Anti-Ero1-Lα is a polyclonal antibody that recognizes the Ero1-Lα protein. Ero1-Lα is an endoplasmic reticulum oxidoreductase involved in the oxidative protein folding process.

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Lab products found in correlation

Anti pdi, by Cell Signaling Technology (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Anti bip, by Cell Signaling Technology (2 mentions) Anti ire1α, by Cell Signaling Technology (2 mentions) Anti calnexin, by Cell Signaling Technology (2 mentions) Anti p erk, by Cell Signaling Technology (2 mentions) Anti β actin, by Merck Group (2 mentions) Anti flag, by Merck Group (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Cell lysis buffer, by Thermo Fisher Scientific (1 mentions) Anti chop, by Cell Signaling Technology (1 mentions) Anti atf6, by Abcam (1 mentions) Anti atg7, by Cell Signaling Technology (1 mentions) Anti beclin 1, by Cell Signaling Technology (1 mentions) Anti gapdh, by Kangchen Biotech (1 mentions) Anti atg5, by Cell Signaling Technology (1 mentions) Anti lc3a b, by Cell Signaling Technology (1 mentions) Anti atg12, by Cell Signaling Technology (1 mentions) Anti atg16l1, by Cell Signaling Technology (1 mentions) Anti p jnk, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Ero1-Lα (17 citations)

4 protocols using anti ero1 lα

1

ER Stress-Induced Apoptosis and Autophagy

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TBT and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cell lysis buffer, fetal bovine serum (FBS), RPMI 1640 medium, and Western Bright Enhanced Chemiluminescence (ECL) detection reagents were obtained from Thermo Fisher Scientific (Suwanee, GA, USA). An Annexin V/PI apoptosis detection kit was purchased from Multi-Sciences (Hangzhou, China). Anti-Bip (Cat#3177), anti-Calnexin (Cat#2679), anti-Ero1-Lα (Cat#3264), anti-IRE1α (Cat#3294), anti-PDI (Cat#3501), anti-CHOP (Cat#2895), anti-PERK (Cat#5683), anti-eIF2α (Cat#5324), anti-P-eIF2α (Cat#3398), anti-Atg12 (Cat#4180), anti-Beclin-1 (Cat#3495), anti-JNK (Cat#3708), anti-P-JNK (Cat#4668), anti-LC3A/B (Cat#12741), anti-Atg5 (Cat#12994), anti-Atg16L1 (Cat#8089), anti-Atg7 (Cat#8558), anti-Atg3 (Cat#3415), anti-rabbit IgG (Cat#7074), and anti-mouse IgG (Cat#7076) were obtained from Cell Signaling Technology (Beverly, MA, USA). Anti-ATF6 (Cat#ab227830) was purchased from Abcam (Cambridge, UK) and anti-GAPDH was obtained from KangChen Biotech (Shanghai, China).
Liang W., Fu L., Feng M., Wang X., Yun Z, & Xu J. (2023). Endoplasmic Reticulum Stress and Autophagy Are Involved in Hepatotoxicity Induced by Tributyltin. Toxics, 11(7), 607.
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2

Western Blot Analysis of Cellular Proteins

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Total cellular protein (10-20 μg) from each sample was separated by SDS-PAGE, transferred to PVDF membranes and subjected to Western blot analysis. Antibodies for Western blot analysis were obtained by the following commercial sources: anti-ubiquitin (Santa Cruz Biotechnology and Millipore), anti-PCNA (Abcam), anti-PARP (BD Pharmingen), anti-GRP78, anti-GCN2, anti-phospho-eIF2oc, anti-IRE1-α, anti-Ero1L-α, anti-caspase-3 (Cell Signaling), anti-β-actin (Sigma). Peroxidase-linked anti-mouse Immunoglobulin G and peroxidase-linked anti-rabbit Immunoglobulin G were from Amersham.
Coughlin K., Anchoori R., Iizuka Y., Meints J., MacNeill L., Vogel R.I., Orlowski R.Z., Lee M.K., Roden R.B, & Bazzaro M. (2014). Small-Molecule RA-9 Inhibits Proteasome-Associated DUBs and Ovarian Cancer in Vitro and in Vivo Via Exacerbating Unfolded Protein Responses. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(12), 3174-3186.
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3

Comprehensive Antibody Validation Protocol

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Anti-Reelin (G10; Millipore; AB_565117), anti-Flag (Sigma-Aldrich; AB_262044), anti-phosphotyrosine (4G10; Millipore; AB_916370), anti-β-actin (Sigma-Aldrich; AB_476744), anti-PA tag (AB_10920577; WAKO), anti-BIP (AB_732737; Abcam), anti-PERK (AB_10831515), anti-PDI (AB_2156433; against PDIA1), anti-Ero1-Lα (AB_823684), anti-phospho-eIF2α (AB_10692650), anti-total-eIF2α (AB_330951; Cell Signaling), and anti-V5 (AB_2556564; Invitrogen). Anti-Dab H1 antibody was a generous gift from Dr. Andre Goffinet (University of Louvain, Belgium).
Lammert D.B., Middleton F.A., Pan J., Olson E.C, & Howell B.W. (2017). The de novo Autism Spectrum Disorder RELN R2290C Mutation Reduces Reelin Secretion and Increases Protein Disulfide Isomerase Expression. Journal of neurochemistry, 142(1), 89-102.
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4

Protein Quantification and Western Blotting

Cited 1 time
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Protein quantification was performed using a BCA Kit. Protein lysates was subjected to SDS-PAGE and subsequently electro-transferred to a polyvinylidene fluoride membrane (Millipore). Western blotting was performed as previously described57 (link), 58 (link). The primary antibodies used were as follows (company, catalog number): anti-ATF6 (Santa Cruz, sc-22799), anti-BIP (Cell Signaling Technology, 3177), anti-P16 (BD Bioscience, 550834), anti-P21 (Cell Signaling Technology, 2947), anti-Lamin B, anti-LAP2 (BD Bioscience, 611000), anti-FOS (Cell Signaling Technology, 2250), anti-Calreticulin (Abcam, ab2907), anti-Calnexin (Cell Signaling Technology, 2679), anti-Ero1Lα (Cell Signaling Technology, 3264), anti-IRE1 (Cell Signaling Technology, 3294), anti-PDI (Cell Signaling Technology, 3501), anti-PERK (Cell Signaling Technology, 5683), anti-EMDM (Santa Cruz, sc-377394), anti-β-Actin (Santa Cruz, sc69879), anti-GAPDH (Santa Cruz, sc-25778), and anti-Flag (Sigma, F1804).
Wang S., Hu B., Ding Z., Dang Y., Wu J., Li D., Liu X., Xiao B., Zhang W., Ren R., Lei J., Hu H., Chen C., Chan P., Li D., Qu J., Tang F, & Liu G.H. (2018). ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells. Cell Discovery, 4, 2.
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