7600 tem

Manufactured by Hitachi

Sourced in United States

The Hitachi 7600 TEM is a high-performance transmission electron microscope (TEM) designed for advanced materials analysis. It features a stable electron optical system and high-resolution imaging capabilities, enabling detailed examination of the microstructure and composition of a wide range of materials.

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Lab products found in correlation

Amtv image capture software, by Advanced Microscopy Techniques (2 mentions) Diatome diamond knife, by Electron Microscopy Sciences (1 mentions) Ultracut e, by Reichert Technologies (1 mentions) Macrofire monochrome progressive scan ccd camera, by Optronics (1 mentions) Eponate 12 resin, by Ted Pella (1 mentions) Ultracut e microtome, by Reichert Technologies (1 mentions) Diamond knife, by Electron Microscopy Sciences (1 mentions) Ultracut e, by Electron Microscopy Sciences (1 mentions)

6 protocols using 7600 tem

Transmission Electron Microscopy of Tissue

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Fresh liver and tissue samples were collected after euthanasia and processed for transmission electron microscopy (TEM) imaging. Tissue pieces (3 mm³) were fixed in 2.0% glutaraldehyde, 2% paraformaldehyde (freshly prepared), 0.1 M sodium cacodylate, 1% sucrose and 3 mM CaCl₂, at pH 7.4 and 4 ºC overnight. The samples were rinsed with buffer and post-fixed in 2% osmium tetroxide with 0.1 M sodium cacodylate and 3 mM calcium chloride on ice protected from light for one hour. After brief washing D-H₂O, the cells were placed in 2% uranyl acetate (0.22 µm filtered) at room temperature in the dark for one hour. Following en-bloc staining, the cells were dehydrated in a dilution series of up to 100% ethanol, transferred through propylene oxide, embedded in Eponate 12 and cured at 60 ºC for two days.
The sections were cut on a Reichert-Jung Ultracut E (Reichert-Jung, Vienna, Austria) with a DiATOME Diamond knife (DiATOME, Hatford, USA). Sections of 80 nm were picked up on formvar coated 1×2 mm copper slot grids. Grids were viewed on a Hitachi 7600 TEM (Hitachi, Berkshire, UK) operating at 80 kV and digital images were captured with an AMT 1 K x 1 K CCD camera.

van Breugel J.M., Geschwind J.F., Mirpour S., Savic L.J., Zhang X., Duran R., Lin M., Miszczuk M., Liapi E, & Chapiro J. (2019). Theranostic application of lipiodol for transarterial chemoembolization in a VX2 rabbit liver tumor model. Theranostics, 9(13), 3674-3686.

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Negative Staining of EVs for TEM

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Negative staining method is used for EV imaging. EVs were fixed in 2% paraformaldehyde for 5 min and loaded on 200 mesh copper grids. TEM was performed at the Electron Microscopy Core of University of Florida on a Hitachi 7600 TEM (Hitachi High-Technologies America) equipped with a MacroFire monochrome progressive scan CCD camera (Optronics).

Yan H., Wen Y., Tian Z., Hart N., Han S., Hughes S.J, & Zeng Y. (2023). A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs. Nature biomedical engineering, 7(12).

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Ultrastructural Analysis of Liver Tissue

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Liver tissues were fixed in 2.5% glutaraldehyde and 4% paraformaldehyde in 100 mM sodium phosphate (pH 7.2). Samples were washed with 100 mM Na cacodylate (pH 7.4), postfixed in 2% osmium tetroxide, and then washed again. The samples were dehydrated in a graded series of ethanol and propylene oxide and embedded in epoxy resin (TAAB 812 Resin; Marivac Industries, Montreal, QC, Canada). Ultrathin (60-70 nm) sections were counterstained with uranyl acetate and lead citrate and viewed using a Hitachi 7600 TEM (Hitachi High-Technologies America, Inc., Schaumburg, IL, USA) equipped with a MacroFire monochrome progressive scan CCD camera (Optronics, Inc., Muskogee, OK, USA) and AMTV image capture software (Advanced Microscopy Techniques, Corp., Danvers, MA, USA).

Wang Y., Zhao T., Deng Y., Hou L., Fan X., Lin L., Zhao W., Jiang K, & Sun C. (2019). Genipin Ameliorates Carbon Tetrachloride-Induced Liver Injury in Mice via the Concomitant Inhibition of Inflammation and Induction of Autophagy. Oxidative Medicine and Cellular Longevity, 2019, 3729051.

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Cardiac Muscle Ultrastructure Analysis

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Thin slices of fresh cardiac muscle were fixed overnight in 2% glutaraldehyde, 0.1 M cacodylate buffer, 3% sucrose and 3 mM CaCl₂ at 4^oC with agitation. Fixed tissues were subjected to 1% osmium tetroxide reduced in potassium ferrocyanide for 1 hour and stained en bloc with 2% aqueous uranyl acetate and serially dehydrated in graded ethanol and propylene oxide. Prepared samples were embedded in Eponate 12 Resin (Ted Pella). Multiple thin sections (70–90 nm) were cut for each sample on a Reichert-Jung Ultracut E microtome, placed on 200 mesh copper grids, stained with uranyl acetate and lead citrate, and viewed on a Hitachi 7600 TEM (Hitachi High Technologies America, Inc) with an AMT Advanced Microscopy Techniques digital camera (Danvers, MA).

Burks T.N., Marx R., Powell L., Rucker J., Bedja D., Heacock E., Smith B.J., Foster D.B., Kass D., O'Rourke B., Walston J.D, & Abadir P.M. (2015). Combined effects of aging and inflammation on renin-angiotensin system mediate mitochondrial dysfunction and phenotypic changes in cardiomyopathies. Oncotarget, 6(14), 11979-11993.

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Ultrastructural Analysis of Liver Tissue

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Liver tissues were fixed in 2.5% glutaraldehyde and 4% paraformaldehyde in 100 mM sodium phosphate (pH 7.2). Samples were washed with 100 mM Na cacodylate (pH 7.4), post-fixed in 2% osmium tetroxide and then washed again. The samples were dehydrated in a graded series of ethanol and propylene oxide and embedded in epoxy resin (TAAB 812 Resin; Marivac Industries, Montreal, QC, Canada). Ultrathin (60–70 nm) sections were counterstained with uranyl acetate and lead citrate and viewed using a Hitachi 7600 TEM (Hitachi High-Technologies America, Inc., Schaumburg, IL, USA) equipped with a Macrofire monochrome progressive scan CCD camera (Optronics, Inc., Muskogee, OK, USA) and AMTV image capture software (Advanced Microscopy Techniques, Corp., Danvers, MA, USA).

Zhao T.M., Wang Y., Deng Y., Fan X.F., Cao X.C., Hou L.J., Mao L.H., Lin L., Zhao W., Wang B.M., Jiang K., Zhao J.W, & Sun C. (2020). Bicyclol Attenuates Acute Liver Injury by Activating Autophagy, Anti-Oxidative and Anti-Inflammatory Capabilities in Mice. Frontiers in Pharmacology, 11, 463.

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Electron Microscopy Imaging of Monolayers

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Monolayers were fixed overnight at 4°C and processed for SEM as previously described^{39 (link)}. Briefly, filters were postfixed in 2% osmium tetroxide and 0.1 μM sodium cacodylate at 4°C for 1h. Filters were dehydrated through a graded series of ethanol and passed through pure HDMS. Drying agent was decanted and filters were desiccated overnight. Filters were attached to aluminum stubs via carbon sticky tabs and coated with 20 nm AuPd. Stubs were viewed and images captured on a Leo 1530 FESEM operating at 1-3 kV.
Monolayers were processed for TEM as previously described^{12 (link)}. Briefly, filters were fixed for 2h in solution containing 2% glutaraldehyde, 2% PFA, 0.1 M Na-cacodylate, 3 mM CaCl₂, pH 7.4 at room temperature. Filters were incubated in 1% H₂O₂ for 1h then post-fixed in 2% osmium tetroxide in 0.1 M Na-cacodylate for 1h then placed in 2% uranyl acetate for 1h. Following en-bloc staining, filters were dehydrated through a graded series of ethanol to 100%, transferred through propylene oxide, embedded in Eponate 12 (Pella) and cured at 60°C for 2d. Sections of 80 nm were cut on a Riechert Ultracut E with a Diatome Diamond knife then collected on formvar coated 1×2 mm copper slot grids and stained with uranyl acetate followed by lead citrate. Grids were viewed and captured on a Hitachi 7600 TEM operating at 80 kV.

In J., Foulke-Abel J., Zachos N.C., Hansen A.M., Kaper J.B., Bernstein H.D., Halushka M., Blutt S., Estes M.K., Donowitz M, & Kovbasnjuk O. (2015). Enterohemorrhagic Escherichia coli Reduces Mucus and Intermicrovillar Bridges in Human Stem Cell-Derived Colonoids. Cellular and Molecular Gastroenterology and Hepatology, 2(1), 48-62.e3.

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