Several urinary cytokines including angiotensinogen (AGT), malondialdehyde (MDA), monocyte chemoat-tractant protein-1 (MCP-1), adiponectin, and podocalyxin were measured. Urinary concentrations of AGT were measured with a Human AGT ELISA Kit (Cat No. CSB-E08564h; Cusabio Biotech, College Park, MD, USA), MDA was measured with MDA assay kits (Cat No. STA-330; Cell Biolab, Inc., San Diego, CA, USA), MCP-1 was measured with a Human MCP-1 ELISA Kit (Quantikine kit; R&D Systems, Abingdon, UK), adiponectin was measured with a highly sensitive ELISA (BioVendor, Brno, Czech), and podocalyxin was measured with human ELISA kit (Cat No. CSB-E09891h; Cusabio Biotech) according to the manufacturers’ protocols. All cytokine concentrations were measured in duplicate and adjusted for urinary creatinine concentration.
Human agt elisa kit
Manufactured by Cusabio
Sourced in United States
The Human AGT ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human Angiotensinogen (AGT) levels in serum, plasma, and other biological fluids. The kit utilizes two specific antibodies that bind to different epitopes of the AGT protein, allowing for its quantification.
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Lab products found in correlation
2 protocols using human agt elisa kit
1
Urinary Cytokine Biomarker Analysis
2
Quantitative AGT ELISA Protocol
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We used a human AGT ELISA kit (Cusabio, Wuhan, China), which employs a quantitative sandwich enzyme immunoassay, to detect serum AGT levels in the case and control groups, following the manufacturer’s instructions. In brief, a microplate was pre-coated with an antibody specific to AGT. Standards and samples were pipetted into individual wells, such that all AGT was bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific to AGT was added. After washing, we added avidin-conjugated horseradish peroxidase to the wells. Following another wash to remove any unbound avidin-enzyme reagent, a substrate solution was added to the wells, which developed color in proportion to the amount of AGT bound in the initial step. After color development stopped, we measured the intensity of the color.
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