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Ovation biotin rna amplification and labelling system

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The Ovation Biotin RNA Amplification and Labelling System is a laboratory equipment product from Tecan. It is designed to amplify and label RNA samples for use in various downstream applications, such as microarray analysis and gene expression studies. The system utilizes a proprietary technology to convert small amounts of total RNA into amplified and biotinylated cDNA, which can then be used for hybridization onto microarray platforms.

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Ovation RNA Amplification System (11 citations) Ovation Biotin System (2 citations)

2 protocols using ovation biotin rna amplification and labelling system

1

T-cell Activation and Transcriptome Analysis

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Cells purified by FACS sorting were stimulated for 2 h with 0.5 μg mL−1 plate‐coated anti‐CD3 (OKT3) and 5 ng mL−1 rhIL‐2 before RNA isolation using the ARCTURUS PicoPure Isolation Kit (ThermoFisher). The concentration of small quantities of RNA was determined using the Nanodrop. Linear amplification of 10 ng of total RNA was performed using the Ovation Biotin RNA amplification and labelling system (NuGEN). Fragmented, labelled cDNA was hybridized to Affymetrix U133 plus 2 arrays.
Callender L.A., Carroll E.C., Beal R.W., Chambers E.S., Nourshargh S., Akbar A.N, & Henson S.M. (2017). Human CD8+ EMRA T cells display a senescence‐associated secretory phenotype regulated by p38 MAPK. Aging Cell, 17(1), e12675.
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2

T Cell Activation and Transcriptional Profiling

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Cells purified by FACS were stimulated for 2 hours with 0.5 μg/ml plate-coated anti-CD3 (OKT3) and 5 ng/ml rhIL-2 before RNA isolation using the ARCTURUS PicoPure Isolation Kit (ThermoFisher). The concentration of small quantities of RNA was determined using Nanodrop. Linear amplification of 10 ng of total RNA was performed using the Ovation Biotin RNA amplification and labelling system (NuGEN). Fragmented, labelled cDNA was hybridized to Affymetrix U133 plus 2 arrays.
Pereira B.I., De Maeyer R.P., Covre L.P., Nehar-Belaid D., Lanna A., Ward S., Marches R., Chambers E.S., Gomes D.C., Riddell N.E., Maini M.K., Teixeira V.H., Janes S.M., Gilroy D.W., Larbi A., Mabbott N.A., Ucar D., Kuchel G.A., Henson S.M., Strid J., Lee J.H., Banchereau J, & Akbar A.N. (2020). Sestrins induce natural killer function in senescent-like CD8+ T cells. Nature immunology, 21(6), 684-694.
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