The MEK inhibitor PD184352, the AKT inhibitor AKTi, Epidermal Growth Factor (EGF), basic Fibroblast Growth Factor (bFGF), Polyethylenimine (PEI), Avidin-FITC and 4′6-diamino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (Rehovot, Israel). Protein A/G PLUS-agarose beads were obtained from Santa Cruz Biotechnology, Inc. (CA, USA). Glutathione beads were purchased from GE-healthcare and Proximity ligation assay kit was from Olink Bioscience (both Uppsala, Sweden). Albumin bovine serum (BSA) was purchased from MP biomedical (OH, USA). Si RNAs and Dharmafect were from Thermo Fisher Scientific (CO, USA). NBT/BCIP developing substrates were purchased from Promega (Wis, USA). Soybean trypsin inhibitor (SBTI) was purchased from Biological Industries (Beit HaEmek, Israel).
Nbt bcip developing substrates
Manufactured by Promega
Sourced in United States
NBT/BCIP developing substrates are colorimetric substrates used in biochemical and molecular biology applications. They are utilized to detect the presence of enzymes, such as alkaline phosphatase, in various assays. These substrates undergo a color change reaction when cleaved by the target enzyme, allowing for visual identification and quantification.
Automatically generated - may contain errors
Lab products found in correlation
Proximity ligation assay kit, by Olink (2 mentions)
Dharmafect, by Thermo Fisher Scientific (2 mentions)
4 6 diamino 2 phenylindole dapi, by Merck Group (2 mentions)
Sirnas, by Thermo Fisher Scientific (2 mentions)
Polyethylenimine (pei), by Merck Group (2 mentions)
Avidin fitc, by Merck Group (1 mentions)
Glutathione beads, by GE Healthcare (1 mentions)
Albumin bovine serum bsa, by MP Biomedicals (1 mentions)
Protein a g plus agarose bead, by Santa Cruz Biotechnology (1 mentions)
Pd184352, by Merck Group (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Tetradecanoyl phorbol acetate, by Merck Group (1 mentions)
Calf intestinal phosphatase, by New England Biolabs (1 mentions)
2 protocols using nbt bcip developing substrates
1
Investigating Cellular Signaling Pathways
2
Molecular Interaction Detection Toolkit
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Tetradecanoyl phorbol acetate (TPA), anisomicin, Polyethylenimine (PEI) and 4'6-diamino-2phenylindole (DAPI) were obtained from Sigma (Rehovot, Israel). A/G beads were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Albumin bovine serum (BSA) was purchased from MP niomedical (Solon, OH, USA). Si RNAs and Dharmafect were from Thermo Fisher Scientific (CO, USA). Proximity ligation assay kit was from Olink Bioscience (Uppsala, Sweden). NBT/BCIP developing substrates were purchased from Promega (WI, USA), Calf Intestinal Phosphatase (CIP) was from New England Biolabs (MA, USA).
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