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Env 203

Manufactured by Med Associates
Sourced in Sao Tome and Principe

The ENV-203 is a compact environmental control unit designed for laboratory applications. It maintains precise temperature and humidity conditions within an enclosed space. The device utilizes advanced thermoelectric technology to provide reliable performance in a space-efficient form factor.

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6 protocols using env 203

1

Operant Conditioning in Rodents

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Animals were trained and tested in operant chambers (ENV-007 Med Associates INC, St. Albans, VT; dimensions: 30.5 cm x 24.1 cm x 21.0 cm) located within light- and sound-attenuating boxes (55.9 cm x 38 cm x 35.6 cm). Each chamber had two retractable levers that were positioned on either side of a food trough, with a house light located on the opposite wall. A pellet dispenser (ENV-203, MED Associates INC) delivered single 45 mg banana-flavored sucrose pellets (Bio-Serv, Frenchtown, NJ) into the food trough. The location of the rewarded lever was assigned randomly to each animal, with presses on the other lever also recorded, but not activating the pellet dispenser. On each training day, rats were placed individually into an operant chamber, with each session beginning with the activation of- and ending with the termination of the house-light. Rats were allowed five minutes to explore the chambers prior to session onset on the day of magazine training, with sessions each day thereafter beginning within one minute after placement into the operant chambers. Following each session, the operant chambers were wiped down with a 6% solution of hydrogen peroxide in tap water, and allowed to dry before the next group of animals was tested.
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2

Automated Rodent Behavior Monitoring

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Twenty-four experiment chambers (Med Associates, dimensions 25 × 30 × 30 cm) were situated in two separate experiment rooms (twelve in each room). Each chamber was contained in a sound-attenuating box (Med Associates, dimensions 74 × 38 × 60 cm) with a fan for ventilation. Each experimental chamber was equipped with a pellet dispenser (Med Associates, ENV-203) on the front wall that delivered the reward into a food cup. A head entry into this cup interrupted a photo beam (Med Associates, ENV-254). On both sides of the food cup, there were two retractable levers. On the opposite wall, a water bottle protruded into the chamber allowing ad libitum access to water during the session. A lick on the spout of the water bottle completed an electric circuit. Four Gateway Pentium III/500 computers running Med-PC for Windows (version 1.15) controlled the experiments and recorded the data. The interruption of the photo beam and the completion of the lick and lever circuits were recorded in time-event format with 2-ms accuracy.
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3

Operant Behavior Testing Platform for Neuroscience Research

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The operant behavior testing (OBT) components were obtained from Med Associates, Inc. (Fairfax, VT): nose poke (ENV-114AM), cue light (ENV-221M), tone generator (ENV-223AM), pellet dispenser (ENV-203) and pellet receptacle (ENV-200R2M). Lithium-Ion batteries (12 V, 10 Ah Model: CR12V10Ah with BMS protection circuit) were purchased from Dakota Lithium Battery (Seattle, WA). An MD-ROOC uses one battery at a time. A bidirectional digital I/O interface (NI 9401), wireless data acquisition unit (cDAQ 9191) and interface software (LabVIEW 2016) originated from National Instruments (Austin, TX). The wireless-LAN router (E1200 N300 Wi-Fi Router) was from Linksys (Irvine, CA). The battery delivers the power for all OBT and National Instruments components. A locally constructed digital interface contains an in-house built printed circuit board that accepts digital inputs to control MedAssociates components as well as receiving signals from the nose poke. (Supplementary Information Figures S.13).
Ordinarily, OBT componentsuse 28 V for both power and control signals. However, they can be configured, or modified, to use 12 V signals instead. Table 1 lists the modifications neccessary to allow the OBT components to be operated at 12 V.
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4

Operant Conditioning Chamber Setup for Behavioral Studies

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Daily experimental sessions were conducted in operant conditioning chambers (modified ENV 007, Med Associates, Inc. St. Albans, VT) housed within sound-attenuating cubicles (ENV-018, Med Associates, Inc.). The chambers contained two levers mounted 5.1 cm above the floor and equidistant from the center of the front wall of the chamber. Pairs of light-emitting diodes (LEDs) were mounted above each lever, and a 28 v d.c. lamp was mounted at the top of the front panel for ambient illumination. A 45-mg food-pellet (Bio-Serv, Inc., Frenchtown, NJ) dispenser (ENV-203, Med Associates, Inc.) was mounted behind the front panel and connected via plastic tubing to a trough contained within a ~5×5 cm opening that was centered between the two levers and accessible to the subject. White noise masked extraneous sounds. A relay mounted behind the front panel produced a click for each response, providing auditory feedback for responses.
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5

Operant Conditioning and Intravenous Injections

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Operant-conditioning chambers (modified ENV-008CT; Med Associates, St. Albans, VT) that measured 25.5 cm × 32.0 cm × 25.0 cm were enclosed within sound-attenuating cubicles equipped with a fan for ventilation and white noise to mask extraneous sounds. Within the chamber, on the front wall, were two response levers, 5.0 cm from the midline and 4.0 cm above the grid floor. A downward displacement of either lever with a force approximating 20 g (0.20 N) produced an audible “feedback” click of a relay mounted behind the front wall of the chamber and defined a response. Three light-emitting diodes (LEDs) were located in a row above each lever. A pellet dispenser (ENV-203; Med Associates) delivered 20-mg sucrose food pellets (Bio-Serv, Flemington, NJ) to a receptacle mounted behind accessible from a 5.0 × 5.0 cm opening in the front wall midway between the two levers and 2.0 cm above the floor.
A syringe driver (model 22; Harvard Apparatus, Holliston, MA) placed above each chamber delivered injections of specified volumes and durations from a 10-ml syringe. The syringe was connected by Tygon tubing to a single-channel fluid swivel (375 Series Single Channel Swivels; Instech Laboratories, Inc., Plymouth Meeting, PA) which was mounted on a balance arm above the chamber. Tygon tubing from the swivel connecting the subject’s catheter was protected by a surrounding metal spring.
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6

Operant Conditioning Chamber Setup for Animal Studies

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Experimental sessions were conducted with animals placed in operant-conditioning chambers (modified ENV-203; MED Associates, St. Albans, VT) that measured 25.5 × 32.05 × 25.5 cm and were enclosed within sound-attenuating cubicles equipped with a fan for ventilation and white noise to mask extraneous sounds. On the front wall of each chamber were two response levers, 5.0 cm from the midline and 4.0 cm above the floor. A downward displacement of the lever with a force equivalent to approximately 20 g defined a response, which always activated a relay mounted behind the front wall of the chamber, producing an audible feedback click. Three light-emitting diodes (LEDs) were located above each lever. A syringe infusion pump (model 22; Harvard Apparatus, Holliston, MA) placed above each chamber delivered injections of specified volumes and durations from a 10 mL syringe. The syringe was connected by Tygon tubing to a single-channel fluid swivel (375 Series Single Channel Swivels; Instech Laboratories, Inc. Plymouth Meeting, PA) that was mounted on a balance arm above the chamber. Tubing from the swivel was connected to the subject’s catheter using a tether provided by Envigo, equipped with a protective metal spring around the tubing.
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