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2 protocols using actinomyces viscosus

1

Multi-species Biofilm Peri-implantitis Model

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The fifteen multi-species biofilm that were used to contaminate the titanium discs are the essential bacterial strains found in peri-implantitis sites. The included bacterial strains were Porphyromonas gingivalis (ATCC 33277), Aggregatibacter actinomycetemcomitans (ATCC43718), Prevotella intermedia (ATCC 25611), Fusobacterium nucleatum (ATCC10953), Streptococcus mutans (ATCC 25175), and Streptococcus sobrinus (ATCC 33478). Commensal bacteria were also included in the study in order to mimic as possible the peri-implant biofilm. The commensal bacteria were Actinomyces viscosus (ATCC 15987), Streptococcus cristatus (ATCC 49999), Actinomyces naeslundii (ATCC 51655), Streptococcus mitis (ATCC 49456), Streptococcus oralis (DSM 20627), Streptococcus sanguinis (LMG 14657), Streptococcus gordonii (ATCC 49818), Veillonella parvula (DSM 2008), and Streptococcus parasanguinis (DSM 6778).
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2

Oral Microbiome Strain Collection

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The following strains commonly present in the oral microbiome (Aas et al., 2005 (link); Maddi and Scannapieco, 2013 (link); Loozen et al., 2014 (link)) were obtained from the American Type Culture Collection (ATCC): A. actinomycetemcomitans (ATCC 43718), F. nucleatum (ATCC 10953), P. gingivalis (ATCC 33277), P. intermedia (ATCC 25611), S. mutans (ATCC 25175), S. sobrinus (ATCC 33478), T. forsythia (ATCC 43037), Actinomyces naeslundii (ATCC 51655), Capnocytophaga sputigena (ATCC 33612), Streptococcus gordonii (ATCC 49818), Actinomyces viscosus (ATCC 15987), and S. mitis (ATCC 49456). Veillonella parvula was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSM 2007); S. sanguinis was acquired from the BCCM/LMG Bacteria Collection (LMG 14657). S. salivarius strain TOVE-R (Tanzer et al., 1985 (link)) was utilized. Bacterial strains were classified as either commensal (indigenous), potentially health-associated or disease-associated, as indicated in Table 1.
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