Alliance ld2 77wl system

Manufactured by Uvitec

Sourced in United Kingdom

The Alliance LD2-77WL system is a laboratory equipment designed for specific analytical applications. It serves as a core function within the research or testing environment, without further interpretation of its intended use.

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Lab products found in correlation

Anti β actin, by Merck Group (3 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (2 mentions) Anti flag m2, by Merck Group (1 mentions) Anti gapdh, by Thermo Fisher Scientific (1 mentions) Anti timp1, by Abcam (1 mentions) Anti p53, by Abcam (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Agilent Technologies (1 mentions) Amersham ecl detection reagent, by GE Healthcare (1 mentions) Anti ilf3, by BD (1 mentions)

Close spelling variants of this product

Alliance Ld2 (8 citations) Alliance system (5 citations) Alliance LD2-77WL imaging system (4 citations)

4 protocols using alliance ld2 77wl system

Western Blot Analysis of Protein Expression

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For Western blot analysis, cell pellets were directly resuspended in Laemli sample buffer, briefly sonicated, boiled and loaded on poly-acrilamide gels.
Primary antibodies used in this study include anti-GFP rabbit polyclonal antibody (Life Techologies, Cat. No. A6445), used 1:1000, anti-FLAG M2 (SIGMA, Cat. No. 3165), 1:1000, anti-β-actin (SIGMA), 1:5000, and anti-TRAF6 (Abnova), used 1:500. To detect endogenous DJ-1 protein an antibody produced in our laboratory was used (Zucchelli et al., 2009 (link); Foti et al., 2010 (link)). For the detection, anti-mouse-HRP or anti-rabbit-HRP (Dako) in combination with ECL (GE Healthcare) was used. Image detection was performed with Alliance LD2-77WL system (Uvitec, Cambridge). Image quantification was done using Adobe Photoshop CS5.

Zucchelli S., Fasolo F., Russo R., Cimatti L., Patrucco L., Takahashi H., Jones M.H., Santoro C., Sblattero D., Cotella D., Persichetti F., Carninci P, & Gustincich S. (2015). SINEUPs are modular antisense long non-coding RNAs that increase synthesis of target proteins in cells. Frontiers in Cellular Neuroscience, 9, 174.

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Western Blot Analysis of Cellular Proteins

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For western blot analysis, cell pellets were resuspended in sample buffer, quickly sonicated, boiled and loaded onto polyacrylamide gels. Anti-GFP rabbit polyclonal antibodies (Life Technologies) and anti-P53 (Abcam) were both used at a dilution of 1:1000. Anti-GAPDH (Invitrogen) was diluted at a ratio of 1:5000, and anti-PTEN (Invitrogen) and anti-TIMP1 (Abcam) were diluted at a ratio of 1:500. ECL was used in conjunction with anti-mouse-HRP or anti-rabbit-HRP (Dako) for detection (GE Healthcare). The Alliance LD2-77WL system (Uvitec, Cambridge) was employed for image detection.

Cao C., Li A., Xu C., Wu B., Liu J, & Liu Y. (2023). Enhancement of protein translation by CRISPR/dCasRx coupled with SINEB2 repeat of noncoding RNAs. Nucleic Acids Research, 51(6), e33.

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Quantitative Western Blot Analysis

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For Western blot analysis, cell pellets were dissolved in Laemmli sample buffer, briefly sonicated, boiled and loaded on 12% poly-acrilamide gels. Immunoblotting was performed with the following primary antibodies: anti-UCHL1 rabbit polyclonal antibody (Millipore, Cat. No. AB1761-I,) 1:5000 and anti-β-actin (SIGMA, Cat. No. A5441), 1:2000. Signals were revealed after incubation with horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, Glostrup, Denmark) in combination with Amersham^™ ECL^™ Detection Reagents (GE Healthcare by SIGMA, Cat. No. RPN2105). Image detection was performed with Alliance LD2-77WL system (Uvitec, Cambridge). Image quantification was done using ImageJ software.

Podbevšek P., Fasolo F., Bon C., Cimatti L., Reißer S., Carninci P., Bussi G., Zucchelli S., Plavec J, & Gustincich S. (2018). Structural determinants of the SINE B2 element embedded in the long non-coding RNA activator of translation AS Uchl1. Scientific Reports, 8, 3189.

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Western Blot and RNA-IP Analysis

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For Western blot analysis, cell pellets were directly dissolved in Laemmli sample buffer. For RNA-IP experiments, ILF3 immunoprecipitation efficiency was monitored by loading the whole fraction of proteins recovered from the organic phase after Trizol extraction, following resuspension in Laemmli sample buffer. All lysates were briefly sonicated, boiled, and loaded on 10% polyacrylamide gels. Immunoblotting was performed with the following primary antibodies: anti-ILF3 (612154; BD Biosciences), 1:500 overnight, and anti–β-actin (A5441; MilliporeSigma), 1:2000. Signals were revealed after incubation with HRP secondary antibodies (Agilent Technologies, Santa Clara, CA, USA) 1:1000 for 1 h at room temperature, in combination with ECL (GE Healthcare). Image detection was performed with Alliance LD2-77WL system (Uvitec, Cambridge, United Kingdom). Image quantification was done using ImageJ software.

Fasolo F., Patrucco L., Volpe M., Bon C., Peano C., Mignone F., Carninci P., Persichetti F., Santoro C., Zucchelli S., Sblattero D., Sanges R., Cotella D, & Gustincich S. (2019). The RNA-binding protein ILF3 binds to transposable element sequences in SINEUP lncRNAs. The FASEB Journal, 33(12), 13572-13589.

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