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Thewta

Manufactured by Merck Group
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TheWTA is a specialized laboratory instrument designed for the detection and measurement of analytes. It employs a proprietary technology to perform high-throughput analysis. The core function of TheWTA is to provide accurate and reliable data to support scientific research and clinical applications.

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Lab products found in correlation

Agilent 8 60 gene chips, by Agilent Technologies (2 mentions) Rneay micro kit, by Qiagen (2 mentions)

2 protocols using thewta

1

Macrophage RNA Isolation and Microarray Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
To isolated RNA, macrophages were directly sorted into QLT buffer
containing 2-mercaptoethanol and RNA isolated using the RNeay micro kit
(Qiagen) per manufacturer’s instructions. Gene expression profiling
was performed using microarray analysis in collaboration with the Genome
Technology Access Core at Washington University. RNA was amplified using the
WTA (Sigma) system and hybridized to Agilent 8×60 gene chips. Data
analysis was performed using Partek genome suite software.
Bajpai G., Schneider C., Wong N., Bredemeyer A., Hulsmans M., Nahrendorf M., Epelman S., Kreisel D., Liu Y., Itoh A., Shankar T.S., Selzman C.H., Drakos S.G, & Lavine K.J. (2018). The Human Heart Contains Distinct Macrophage Subsets with Divergent Origins and Functions. Nature medicine, 24(8), 1234-1245.
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2

Macrophage RNA Isolation and Microarray Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
To isolated RNA, macrophages were directly sorted into QLT buffer
containing 2-mercaptoethanol and RNA isolated using the RNeay micro kit
(Qiagen) per manufacturer’s instructions. Gene expression profiling
was performed using microarray analysis in collaboration with the Genome
Technology Access Core at Washington University. RNA was amplified using the
WTA (Sigma) system and hybridized to Agilent 8×60 gene chips. Data
analysis was performed using Partek genome suite software.
Bajpai G., Schneider C., Wong N., Bredemeyer A., Hulsmans M., Nahrendorf M., Epelman S., Kreisel D., Liu Y., Itoh A., Shankar T.S., Selzman C.H., Drakos S.G, & Lavine K.J. (2018). The Human Heart Contains Distinct Macrophage Subsets with Divergent Origins and Functions. Nature medicine, 24(8), 1234-1245.
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