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Rotina 380

Manufactured by Hettich
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Sourced in Germany, Switzerland

The Rotina 380 is a centrifuge designed for general laboratory use. It features a maximum speed of 4,000 rpm and a maximum relative centrifugal force (RCF) of 3,220 x g. The Rotina 380 can accommodate a variety of rotor sizes and sample volumes.

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Lab products found in correlation

Cobas c501, by Roche (1 mentions) Unimax 2010, by Heidolph (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Peg 10 000, by Merck Group (1 mentions) Zn no3 2 6h2o, by Merck Group (1 mentions) Cu no3 2 3h2o, by Carl Roth (1 mentions) Universal 320r, by Hettich (1 mentions) Auw220d, by Shimadzu (1 mentions)

Spelling variants of this product

Rotina 380 R (46 citations) Rotina 380 R Benchtop (2 citations)

12 protocols using rotina 380

1

Vitamin D and Calcium Serum Analysis

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Blood samples were drawn from the antecubital vein using a blood collection system (S-Monovette® 4.9 mL Z, Sarestedt, Nümbrecht, Germany). Samples were immediately packed into an opaque plastic tube to protect them from any ultraviolet radiation. The samples were then immediately centrifuged at 20 °C at 3000 rpm for 10 min (Rotina 380, Hettich GmbH, Tuttlingen, Germany) in the in-house laboratory of the Swiss Paraplegic Centre, Nottwil, Switzerland. After centrifugation, the samples were stored at −25 °C for later analysis.
Serum 25-hydroxyvitamin D (25[OH]D) was analyzed with an automated benchtop immunoanalyzer (Vidas®, bioMérieux, Marcy l’Etoile, France) using enzyme-linked fluorescent assay (ELFA). Serum calcium concentration was assayed with a photometric technique method (Cobas c501, Roche Diagnostic GmbH, Mannheim, Germany).
Flueck J.L., Schlaepfer M.W, & Perret C. (2016). Effect of 12-Week Vitamin D Supplementation on 25[OH]D Status and Performance in Athletes with a Spinal Cord Injury. Nutrients, 8(10), 586.
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2

Optimizing Microbial Lipid Production

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Chemicals were purchased from Sigma-Aldrich and Fisher Scientific and used without further purification. Centrifugations took place at 4667 × g and room temperature for 10 min (Rotina 380, Hettich) . All cultivations took place at 20 °C (Abeln et al., 2019; Santomauro et al., 2014) .
Erlenmeyer flasks were filled at 20 % (v/v) working volume and placed on orbital shakers (Unimax 2010, Heidolph) at 180 rpm. Rates were calculated as average between two sample points (typically daily). The lipid flux was expressed as lipid production rate (rP) over lipid-free biomass production rate (rX*).
Abeln F, & Chuck C.J. (2019). Achieving a high-density oleaginous yeast culture: Comparison of four processing strategies using Metschnikowia pulcherrima. Biotechnology and bioengineering, 116(12).
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3

Efficient EV Depletion from FBS

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FBS (Gibco) was depleted from EVs using ultracentrifugation (for breast cancer cell MCF7 experiments) or polyethylene glycol (PEG 10000) (for melanoma cell A2058 experiments). Shortly, the EV‐depleted FBS was prepared by ultracentrifugation at 110,000 × g for 16 h. After centrifugation, the supernatant was collected and sterile‐filtered (0.22 μm). A PEG 10000 (Sigma‐Aldrich, USA) 50% (w/v) stock solution was prepared in Dulbecco's phosphate buffered saline (dPBS) [sterile filtered (0.2 μm)]. The PEG stock solution was stored protected from light and at 4°C. The FBS and PEG stock solution were mixed in a 5:1 ratio by gently inverting 5–10 times and incubated for 2 h at 4°C protected from light. After, the mix of PEG‐FBS was centrifugated for 30 min at 4°C at 1500 × g in a swinging‐bucket rotor (four place, angle 90°, Hettich, Germany) in benchtop centrifuge (Rotina 380, Hettich, Germany). The supernatant of the PEG‐FBS solution was collected leaving a layer of at least 0.5 cm on top of the pellet, and again sterile filtered (0.1 μm) into aliquots stored at –20°C until used (Laukkanen et al., 2020 (link)).
Kyykallio H., Faria A.V., Hartman R., Capra J., Rilla K, & Siljander P.R. (2022). A quick pipeline for the isolation of 3D cell culture‐derived extracellular vesicles. Journal of Extracellular Vesicles, 11(10), 12273.
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4

Coprecipitation of Cu-Zn Oxalate Precursors

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A 1.0 M aqueous solution containing the desired proportions of Cu(NO3)2•3 H2O (>99 %, Carl Roth) and Zn(NO3)2•6 H2O (>99 %, Sigma-Aldrich) was prepared. The pH was adjusted to 0.5 by addition of HNO3 (>65 wt %, Carl Roth). The precipitating agent was aqueous 1.2 M K2C2O4•H2O (>98 %, Carl Roth). The reaction vessel was filled with Millipore water (50 mL). With strict control of pH (pH 1.0) and temperature (303 K), the metal salt solution was added dropwise over 20 min with vigorous stirring. To keep the pH constant at 1.0, appropriate amounts of K2C2O4 solution was added during the coprecipitation step. The precipitation was followed by an aging time of 5 min (303 K, pH 1.0). Afterwards, precipitate was collected by filtration or centrifugation (Rotina 380, Hettich) and washed several times with Millipore water until the conductivity of the washing medium was <0.5 mS cm -1 . The solid was dried at 353 K in air overnight and stored in a desiccator over silica gel orange.
Zwiener L., Girgsdies F., Schlögl R, & Frei E. (2018). Investigations of Cu/Zn Oxalates from Aqueous Solution: Single-Phase Precursors and Beyond. Chemistry (Weinheim an der Bergstrasse, Germany), 24(56).
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5

Vacuum Freeze Drying Purification Protocol

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The vacuum freeze dryer (Christ-Alpha, Osterode, Germany), ultrasonic cleaner (DTC-27, Suzhou City, China), ultrapure water preparation machine (Millipore-Q, Bedford, MA, USA), centrifuge (Hettich Rotina-380, Tuttlingen, Germany), Nanodrop (Thermo-2000, Waltham, MA, USA), gel imaging system (Thermo-Gene, Waltham, MA, USA), 0.2 μm filter membrane, sand core funnel, shaker, centrifuge tube and other instrument consumables were all domestic. Other instruments included a high-performance liquid chromatograph (LC2020, Shimadzu, Kyoto, Japan) and chromatographic column (Shimadzu, Kyoto, Japan, SB-C18, 4.6 × 250 mm, 5 μm).
An agarose gel recovery kit, PCR mix, markers, sterile enzyme-free water, PBS solution, ethanol, sodium hypochlorite, Tween 80, and liquid nitrogen were also used.
Liu C., Lin H., Wang K., Zhang Z., Huang J, & Liu Z. (2023). Study on the Trend in Microbial Changes during the Fermentation of Black Tea and Its Effect on the Quality. Foods, 12(10), 1944.
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6

Lipid and Moisture Content of Fish

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The lipid and moisture content of fish homogenate samples was determined by gravimetric methods according to our work [19 (link)]. For total lipid determination, 5 g of fish homogenate was extracted with 5 mL of acetone/ethyl acetate solvent mixture (6:4, v/v) by shaking with a vortex mixer (Stuart SA8, Bibby Scientific, Stone, UK) for 3 min and, after addition of 2 g of MgSO4 and 0.5 g NaCl and shaking for 3 min, the organic phase was separated by centrifugation (centrifuge Rotina 380, Hettich, Tuttlingen, Germany). An aliquot of the organic phase was dried to constant weight at 103 °C, and the percent lipid content was calculated from the mass of the final residue. The moisture content was determined from the mass difference of 2–3 g portions of fish homogenate before and after a 24 h drying at 60 °C. For all fish sample homogenates, the lipid content was determined in triplicates (results in the range 0.63–16%) and the moisture content in duplicates (58–81%).
Nagyová S, & Tölgyessy P. (2019). Validation Including Uncertainty Estimation of a GC–MS/MS Method for Determination of Selected Halogenated Priority Substances in Fish Using Rapid and Efficient Lipid Removing Sample Preparation. Foods, 8(3), 101.
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7

Direct Ink Writing of Cellulose Nanostructures

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TEMPO-oxidized CNF and CNC were printed using a direct ink writing (DIW) Ultimaker 2+ instrument (my3DWorld GmbH, Switzerland) enclosed in a plexiglass enclosure to maintain sterile conditions during printing steps. The cellulose ink was loaded into a sterile 20 mL plastic Luer-lock syringe inside a laminar flow-hood using a sterile spatula. The syringe was capped with an ethanol sterilized syringe barrel end cap (S&P Shop, H. Sigrist & Partner AG, Switzerland) and then centrifuged for 5 to 10 min at 3,000 rpm (Rotina 380, Hettich AG, Switzerland) before printing. At the printing step, the end cap was replaced with an ethanol-sterilized, 0.84 mm diameter, tapered dispensing tip (S&P Shop, H. Sigrist & Partner AG, Switzerland). Printing was carried out at room temperature. The printing platform and the plexiglass enclosure were sterilized with 70% ethanol before printing.
Reyes C., Sajó Z., Lucas M.S., Sinha A., Schwarze F.W., Ribera J, & Nyström G. (2022). Cocultivation of White-Rot Fungi and Microalgae in the Presence of Nanocellulose. Microbiology Spectrum, 10(5), e03041-22.
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8

Direct Ink Writing of Cellulose Nanostructures

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TEMPO-oxidized CNF and CNC were printed using a direct ink writing (DIW) Ultimaker 2+ instrument (my3DWorld GmbH, Switzerland) enclosed in a plexiglass enclosure to maintain sterile conditions during printing steps. The cellulose ink was loaded into a sterile 20 mL plastic Luer-lock syringe inside a laminar flow-hood using a sterile spatula. The syringe was capped with an ethanol sterilized syringe barrel end cap (S&P Shop, H. Sigrist & Partner AG, Switzerland) and then centrifuged for 5 to 10 min at 3,000 rpm (Rotina 380, Hettich AG, Switzerland) before printing. At the printing step, the end cap was replaced with an ethanol-sterilized, 0.84 mm diameter, tapered dispensing tip (S&P Shop, H. Sigrist & Partner AG, Switzerland). Printing was carried out at room temperature. The printing platform and the plexiglass enclosure were sterilized with 70% ethanol before printing.
Reyes C., Sajó Z., Lucas M.S., Sinha A., Schwarze F.W., Ribera J, & Nyström G. (2022). Cocultivation of White-Rot Fungi and Microalgae in the Presence of Nanocellulose. Microbiology Spectrum, 10(5), e03041-22.
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9

Fibroin Viscosity Modulation Protocol

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The lyophilized fibroin was weighed on a precision balance (Discovery SeriesDV215CDM from Ohaus). Afterward, small pieces of fibroin were added to a predetermined volume of deionized water while slow circular movements were performed to avoid the formation of surface scum in the sample. The solution was placed in a centrifuge (Rotina 380 from Hettich) at 5000 rpm and 24ºC for10 minutes to induce the decantation of non-dissolved pieces of fibroin. Finally, the fibroin solution was deposited into a new container and stored in the fridge at 8ºC. A total of three fibroin samples in which the viscosity was modified by changing the solute concentrations (0.062, 0.075 and 0.125 g/mL) were prepared. The viscosity values (η=3.31, η=4.12 and η=7.97mPa.s) were obtained at room temperature using a rotational viscometer (Alpha Series from Fungilab). It is worth noting that one of the viscosities (η=4.12mPa.s) was used as a phantom system that mimicked the biological-media viscosity of cytoplasm (η=4 mPa.s) [20] .
Urbano-Bojorge A.L., Casanova-Carvajal O., Félix-González N., Fernández L., Madurga R., Sánchez-Cabezas S., Aznar E., Ramos M, & Serrano-Olmedo J.J. (2018). Influence of medium viscosity and intracellular environment on the magnetization of superparamagnetic nanoparticles in silk fibroin solutions and 3T3 mouse fibroblast cell cultures. Nanotechnology, 29(38).
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10

Oxidation of Microcrystalline Cellulose

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Microcrystalline cellulose was suspended in an aqueous solution (deionized water) of sodium metaperiodate with a 1.25 molar ratio between the oxidant and cellulose (anhydroglucose unit). The suspension was stirred for 24 h in the dark at 35 °C. The product was separated by centrifugation (5000 rpm for 20 min, Hettich Rotina 380, Westphalia, Germany) and washed. The never-dried DAC was suspended in water (5% of solid content) and heated to 100 °C for 90 min for solubilization [36 (link)]. The pH was adjusted to 3.5 with acetic acid. Determination of the aldehyde content was performed with the oxime titration method, as reported in the literature [37 (link)].
Lucia A., Bacher M., van Herwijnen H.W, & Rosenau T. (2020). A Direct Silanization Protocol for Dialdehyde Cellulose. Molecules, 25(10), 2458.
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