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Ebiosience

Manufactured by Thermo Fisher Scientific

The Thermo Fisher Scientific EBioscience is a line of laboratory equipment and reagents designed for use in life science research and diagnostics. The core function of the EBioscience product line is to provide researchers with high-quality tools for analysis, detection, and quantification of various biomolecules and cellular components. The specific features and capabilities of individual EBioscience products may vary, and further details would require a more detailed review of the specific item in question.

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2 protocols using ebiosience

1

Measuring Inflammatory Factors by ELISA

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The levels of inflammatory factors in the cell supernatant were measured by TNF-α, IL-6 and IL-1β ELISA kits (cat. nos. BMS223-4, BMS213-2 and BMS224-2, respectively; eBiosience; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol.
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2

Multiparametric Flow Cytometry Analysis of Immune Cell Phenotypes

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The B cells isolated from the spleen or MLNs were stained with APC anti‐CD19 antibody (6D5, #115512) and then fixed and permeabilized with the fixation/ permeabilization buffer (Biolegend) according to the manufacturer's protocol. Permeabilized cells were stained with FITC anti‐IL‐10 antibody (JES5‐16E3, #505006). To identify CD4+ T cell polarization, APC anti‐CD3 antibody (17A2, #100236), FITC anti‐CD4 antibody (GK1.5, #100406), PE‐anti‐IL‐4 antibody (11B11, #504104), PE‐anti‐IFN‐γ antibody (XMG1.2, #505808) and PerCP‐Cy5.5 anti‐IL‐17A antibody (TC11‐18H10.1, #506920) were used for detection of intracellular cytokine expression. All antibodies used in FCM analysis were purchased from Biolegend and eBiosience (Thermo Fisher Scientific).
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