The following antibodies were used for ChIP-seq: rabbit polyclonal anti-HIF-2α (Novus Biologicals, Centennial, CO, #NB100-122), mouse monoclonal anti-HIF-1β (Novus Biologicals, #NB100-124), rabbit polyclonal anti-V5 (Abcam, Cambridge, UK, #ab9116). The following antibodies were used for Cut&Run: mouse monoclonal anti-V5 tag (Thermo Fisher, #R960-25) diluted to 0.01 mg/ml, mouse IgG (Jackson ImmunoResearch #015-000-003) diluted to 0.01 mg/ml, rabbit anti-mouse IgG H&L (Abcam, #ab46540) diluted to 0.01 mg/ml. The following antibodies were used for western blotting: rabbit monoclonal anti-HIF-2α (Cell Signaling, Danvers, MA, #D9E3) diluted at 1:1000, rabbit monoclonal anti-HIF-1β (Cell Signaling, #D28F3) diluted at 1:1000, mouse monoclonal anti-V5 tag (Thermo Fisher, #R960-25) diluted at 1:2500, mouse monoclonal anti-HaloTag (Promega, Madison, WI, #G9211) diluted at 1:1000, mouse monoclonal anti-TBP (Abcam, #ab51841) diluted at 1:2500, goat-anti-mouse-HRP (Thermo Fisher, #31430) diluted at 1:2000, goat-anti-rabbit-HRP (Thermo Fisher, #31462) diluted at 1:2000.
Mouse monoclonal anti halotag
Manufactured by Promega
The Mouse monoclonal anti-HaloTag is a laboratory reagent used to detect the presence of the HaloTag protein in biological samples. It is a mouse-derived monoclonal antibody that specifically binds to the HaloTag protein, allowing for the identification and quantification of HaloTag-labeled proteins.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit polyclonal anti v5, by Abcam (2 mentions)
Goat anti mouse hrp, by Thermo Fisher Scientific (2 mentions)
Goat anti rabbit hrp, by Thermo Fisher Scientific (2 mentions)
Rabbit polyclonal anti hif 2α, by Novus Biologicals (2 mentions)
Mouse monoclonal anti hif 1β, by Novus Biologicals (2 mentions)
Mouse monoclonal anti v5 tag, by Thermo Fisher Scientific (2 mentions)
Rabbit monoclonal anti hif 2α, by Cell Signaling Technology (2 mentions)
Rabbit monoclonal anti hif 1β, by Cell Signaling Technology (2 mentions)
Mouse monoclonal anti tbp, by Abcam (2 mentions)
Rabbit anti mouse igg h l, by Abcam (1 mentions)
2 protocols using mouse monoclonal anti halotag
1
Antibody validation for HIF-1/2α ChIP-seq and Western blot
2
Antibodies for ChIP-seq and Western Blotting
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The following antibodies were used for ChIP-seq: rabbit polyclonal anti-HIF-2α (Novus Biologicals, Centennial, CO, #NB100-122), mouse monoclonal anti-HIF-1β (Novus Biologicals, #NB100-124), rabbit polyclonal anti-V5 (Abcam, Cambridge, UK, #ab9116). The following antibodies were used for western blotting: rabbit monoclonal anti-HIF-2α (Cell Signaling, Danvers, MA, #D9E3) diluted at 1:1000, rabbit monoclonal anti-HIF-1β (Cell Signaling, #D28F3) diluted at 1:1000, mouse monoclonal anti-V5 tag (ThermoFisher, # R960-25) diluted at 1:2500, mouse monoclonal anti-HaloTag (Promega, Madison, WI, # G9211) diluted at 1:1000, mouse monoclonal anti-TBP (Abcam, #ab51841) diluted at 1:2500, goat-anti-mouse-HRP (ThermoFisher, #31430) diluted at 1:2000, goat-anti-rabbit-HRP (ThermoFisher, # 31462) diluted at 1:2000.
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