Immunocytochemistry was performed to determine the nuclear distribution of γH2AX in individual cells. The cells were grown on chambered slides 1 day prior to the TTF treatment. The TTF treatment was performed for 48 h while the cells remained attached to the slides, followed by fixation with 4% paraformaldehyde and permeabilization with 0.5% Triton X-100 in PBS. The detection was performed after blocking the slides in 10% FBS/1% bovine serum albumin for 1 h with a 1:1000 dilution of a FITC-labeled mouse monoclonal antibody against γH2AX (Millipore, Billerica, MA, USA).
Fitc labeled mouse monoclonal antibody against γ h2ax
Manufactured by Merck Group
Sourced in United States
FITC-labeled mouse monoclonal antibody against γ-H2AX. This product is a fluorescently-labeled antibody directed against phosphorylated histone H2AX, a marker of DNA double-strand breaks.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using fitc labeled mouse monoclonal antibody against γ h2ax
1
Quantifying DNA Damage via γH2AX
2
Quantifying Nuclear γ-H2AX Distribution
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Immunocytochemistry was performed to determine the nuclear distribution of γ-H2AX in individual cells. Cells were grown on chambered slides for 1 day prior to IR. After exposure, cells were irradiated and incubated for 1 or 24 h. All treatments were performed while cells remained attached to the slides, followed by fixation with 4% (w/v) paraformaldehyde and permeabilization with 0.5% (v/v) Triton™ X-100 in PBS. Detection was performed after the slides were blocked in 10% (v/v) FBS/1% (v/v) bovine serum albumin for 1 h, followed by incubation with a 1:1,000 dilution of FITC-labeled mouse monoclonal antibody against γ-H2AX (Millipore).
3
Immunocytochemical Analysis of γ-H2AX
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Immunocytochemistry was performed to determine the nuclear distribution of γ-H2AX in individual cells. Cells were grown on chambered slides for 1 day prior to IR or ZOL treatment. After 24 h ZOL exposure, cells were irradiated and treated for 1 h or 24 h. All treatments were performed while cells remained attached to the slides, followed by fixation with 4% (w/v) paraformaldehyde and permeabilization with 0.5% (v/v) Triton™ X-100 in PBS. Detection was performed after the slides were blocked in 10% (v/v) FBS/1% (v/v) bovine serum albumin for 1 h with a 1:1000 dilution of FITC-labeled mouse monoclonal antibody against γ-H2AX (Millipore).
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