The Chiralpak AS-H is a chiral HPLC column designed for the separation of enantiomers. It is based on amylose tris(3,5-dimethylphenylcarbamate) as the chiral stationary phase. The column is available in various sizes and dimensions to meet different analytical requirements.
Example 72
The stereoisomers of Example 71 (51 mg) prepared as described above are separated by HPLC using a chiral stationary phase to give 16.5 mg of stereoisomer 1 (Example 72) and 16.4 mg of stereoisomer 2 (Example 73).
Method for Separation:
HPLC apparatus type: Waters 600 Pump, 2767 Autosampler, UV Detector 2489; column: Daicel Chiralpak AS-H, 5.0 μm, 250 mm×20 mm; method: eluent hexane/ethanol 90:10; flow rate: 15 mL/min, temperature: 25° C.; UV Detection: 230 nm
Example 67
The stereoisomers of Example 66 (109 mg) prepared as described above are separated by HPLC using a chiral stationary phase to give 12.4 mg of stereoisomer 1 (Example 67) and 12.6 mg of stereoisomer 2 (Example 68).
Method for Separation:
HPLC apparatus type: Waters 600 Pump, 2767 Autosampler, UV Detector 2489; column: Daicel Chiralpak AS-H, 5.0 μm, 250 mm×20 mm; method: eluent hexane/ethanol 65:35; flow rate: 15 mL/min, temperature: 25° C.; UV Detection: 230 nm
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