To test motility on plates, 3μl of log-phase cells were spotted onto semi-solid agar (1% Tryptone, 0.5% Sodium Chloride, 0.25% Agar), and grown 7 hours at 37°C under green (525nm) or red light (650nm). To observe cell motility by microscopy, 10 μl of cell culture was mounted on a glass slide by placing the drop between two #1.5 coverslips, then covering it with a third #1.5 coverslip. Videos of motile and non-motile cells were recorded using the streaming mode of a Hamamatsu Orca-Flash4.0 sCMOS camera mounted on a Zeiss Axio Imager Z2 epifluorescence microscope, viewed through a Plan-Apochromat, 20x/0.18 Oil Ph1 objective. Phase contrast and fluorescence movies (filter 63HE) consisted of 500 image frames, acquired continuously with 30ms exposure times, and were acquired independently but in rapid succession, focused on the same field. The TrackMate plugin for ImageJ was used to analyze these movies in order to track cell motility.
Orca flash4.0 scmos camera
Manufactured by Zeiss
The Orca-Flash4.0 sCMOS camera is a scientific CMOS (sCMOS) camera produced by Zeiss. It features a high-resolution sensor with a large field of view and fast readout speeds. The camera is designed for various applications in scientific research and imaging.
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Lab products found in correlation
2 protocols using orca flash4.0 scmos camera
1
Visualizing Microbial Motility Dynamics
2
Visualizing Microbial Motility Dynamics
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To test motility on plates, 3μl of log-phase cells were spotted onto semi-solid agar (1% Tryptone, 0.5% Sodium Chloride, 0.25% Agar), and grown 7 hours at 37°C under green (525nm) or red light (650nm). To observe cell motility by microscopy, 10 μl of cell culture was mounted on a glass slide by placing the drop between two #1.5 coverslips, then covering it with a third #1.5 coverslip. Videos of motile and non-motile cells were recorded using the streaming mode of a Hamamatsu Orca-Flash4.0 sCMOS camera mounted on a Zeiss Axio Imager Z2 epifluorescence microscope, viewed through a Plan-Apochromat, 20x/0.18 Oil Ph1 objective. Phase contrast and fluorescence movies (filter 63HE) consisted of 500 image frames, acquired continuously with 30ms exposure times, and were acquired independently but in rapid succession, focused on the same field. The TrackMate plugin for ImageJ was used to analyze these movies in order to track cell motility.
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