Flow cytometry experiments were performed on a CytoFLEX V2-B4-R2 Flow Cytometer (eight detectors, three lasers) (Beckman-Coulter, Germany). Antibodies used for surface staining are listed in ESM Table 1 . For measuring intracellular thiol levels, ThiolTracker dye (T10095, Thermo Fisher Switzerland), was used according to the manufacturer’s directions. Cells were washed and subsequently analysed.
Thioltracker dye
Manufactured by Thermo Fisher Scientific
Sourced in Switzerland
ThiolTracker dye is a fluorescent probe used to detect and quantify thiol-containing molecules in biological samples. It binds reversibly to reduced thiols, allowing for the visualization and measurement of thiol levels in cells and tissues.
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Lab products found in correlation
2 protocols using thioltracker dye
1
Flow Cytometry Analysis of Cell Thiols
2
Flow Cytometry Analysis of Macrophages
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Flow cytometry experiments were performed on a CytoFLEX V2-B4-R2 Flow Cytometer (8 Detectors, 3 Lasers) (Beckman Coultier, Germany). In short, cells were harvested as described above and subsequently stained with the appropriate antibodies and dyes. For flow cytometry experiments, BMDMs or peritoneal lavage was harvested. F4/80 APC-Cy7 (Cat nr. 123113, Biolegend, UK) and CD11b PE-Cy7 (Cat nr. 101215, Biolegend, UK) positive cells were considered macrophages.
For measuring intracellular thiol levels, Thioltracker dye (T10095, Thermo Fisher Switzerland), was used according to manufacturer’s directions.
For measuring intracellular thiol levels, Thioltracker dye (T10095, Thermo Fisher Switzerland), was used according to manufacturer’s directions.
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