MCF7 or MDA-MB-231 cell lysates were resolved by 12% SDS–PAGE, electrotransferred to polyvinylidene difluoride membrane followed by immunoblotting using antibodies against PDCD4 (Cell Signaling Technology), HuR, La/SSB, and GAPDH (Santacruz Biotechnology) as primary antibodies, and horseradish peroxidase-conjugated β-Actin (Genscript) or anti-mouse or anti-Rabbit (Cell Signaling Technology) as secondary antibodies according to the primary antibodies used. The bands were detected using Femtolucent chemiluminescence detection kit (G-Biosciences).
Femtolucent chemiluminescence detection kit
Manufactured by G Biosciences
Sourced in United States
The Femtolucent chemiluminescence detection kit is a laboratory product designed for the detection and quantification of chemiluminescent signals. It is a sensitive tool used for various applications in life science research, such as protein and nucleic acid analysis.
Automatically generated - may contain errors
2 protocols using femtolucent chemiluminescence detection kit
1
Immunoblotting for PDCD4, HuR, and La/SSB
2
Immunoblotting Analysis of PDCD4, HuR, and Myc
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Equal amounts of cell lysates were resolved by 12% SDS–PAGE, electro-transferred to polyvinylidene difluoride membrane followed by immunoblotting using antibodies against PDCD4 (Cell Signaling Technology, Danvers, MA, USA), HuR (Santacruz Biotechnology, Dallas, TX, USA), GAPDH (Santacruz Biotechnology), myc (Cell Signaling Technology) and horseradish peroxidase-conjugated β-Actin (Genscript, Piscataway, NJ, USA) or anti-Mouse (Cell Signaling Technology) or anti-Rabbit (Cell Signaling Technology) secondary antibodies. The bands were detected using Femtolucent chemiluminescence detection kit (G-Biosciences, St Louis, MO, USA).
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