Zebrafish at 7 dpf were fixed in 4% paraformaldehyde in PBS for 1 h at room temperature. The fish were washed with PBS subsequently to remove paraformaldehyde, incubated in 60% 2-propanol for 10 min, followed by freshly filtered 0.3% oil red O (ORO) (Xiya Reagent, Chengdu, China) in 60% 2-propanol for 10 min. The staining was terminated by washing twice with 60% 2-propanol. The samples were transferred to 75% glycerol and imaged using Lecia M205 FCA (Lecia Wetzlar, Germany).
Lecia m205 fca
Manufactured by Leica
Sourced in Germany
The Leica M205 FCA is a stereo microscope designed for high-performance imaging and analysis. It features a fully apochromatic optical system, providing high-quality, low-distortion images with excellent color fidelity. The microscope is equipped with various magnification options and can be adapted for a range of applications, including materials science, quality control, and precision engineering.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lecia m205 fca
1
Lipid Droplet Visualization in Zebrafish
2
Isolation of Zebrafish Pancreatic Islets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seven days postfertilization, Tg(gcga:GFP) and gcgr−/−;Tg(gcga:GFP) zebrafish larvae were sacrificed and digested gently with collagenase P (1.2 mg/ml, Sigma–Aldrich) for 5 min at 37 °C, then the islets were hand-picked using forceps, from the homogenate under a fluorescence microscope (Lecia M205 FCA, Lecia Wetzlar). The isolated islets were further digested to a single cell suspension with Liberase DH (100 μg/ml, Roche) for 50 min at 36 °C. Finally, the GFP-positive cells were collected by fluorescence-activated cell sorting (BD AriaIII) into 96-well plate containing lysis buffer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!