Spinning disk confocal imaging was performed with a Quorum spinning disk confocal head on a Zeiss Axiovert 200M microscope, equipped with a 63× NA 1.4 oil objective and 25× NA 0.8 multi-immersion objective, a 1.5× tube lens, and a back-thinned EM-CCD camera (C9100–13, Hamamatsu). Acquisition settings were controlled using Volocity software and were acquired at equal laser and exposure settings within experiments across compared conditions. Lightning microscopy was performed on a Leica SP8 Lightning Confocal DMI6000 microscope utilizing a 100× 1.4 NA (O, STED) objective and two HyD detectors. Acquisitions were driven by a Leica motorized XY stage and Leica SuperZ Galvo with Adaptive Focus Control. Acquisition settings were controlled using the Leica LAS, Lightning Module software, with matched settings within experiments.
Sp8 lightning confocal dmi6000 microscope
Manufactured by Leica
The SP8 Lightning Confocal DMI6000 microscope is a high-performance confocal imaging system designed for advanced microscopy applications. It features a modular design, allowing for customization to meet specific research needs. The microscope provides high-resolution imaging capabilities, enabling the visualization and analysis of biological samples at the cellular and subcellular levels.
Automatically generated - may contain errors
Lab products found in correlation
Axiovert 200m microscope, by Zeiss (2 mentions)
Back thinned em ccd camera, by Hamamatsu Photonics (2 mentions)
Motorized xy stage, by Leica (2 mentions)
Superz galvo, by Leica (2 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Anti cathepsin b, by Cell Signaling Technology (1 mentions)
Anti stem121, by Takara Bio (1 mentions)
Anti phospho histone h3, by Cell Signaling Technology (1 mentions)
Anti cd44, by Abcam (1 mentions)
Anti γh2ax, by Cell Signaling Technology (1 mentions)
3 protocols using sp8 lightning confocal dmi6000 microscope
1
Spinning Disk and Lightning Confocal Imaging
2
Comprehensive Tissue Analysis via IHC and H&E
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Immunohistochemistry and H&E staining were performed on cryo- or paraffin-embedded tissue sections. Primary antibodies were anti-STEM121 (1:100; Takara Bio Inc., Y40410), anti-cleaved Caspase-3 (1:200; Cell Signaling Technology, 9661), anti-cathepsin B (1:200; Cell Signaling Technology, 31718S), anti-γH2Ax (1:500; Cell Signaling Technology, 2577S), anti–phospho-Histone H3 (1:200; Cell Signaling Technology, 9706S), and anti-CD44 (1:200; Abcam, ab243894). Secondary antibodies conjugated to Alexa Fluor dyes (488, 555, and 647) at a dilution of 1:200 were used. Terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling kit (Sigma-Aldrich, cat. no. S7110,) was used for apoptosis quantification. DAPI (Sigma-Aldrich, cat. no. D9564) was used for nuclear counterstain. Images were acquired using a Leica SP8 Lightning Confocal DMI6000 microscope. Images were analyzed using Imaris software.
3
Spinning Disk and Lightning Confocal Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Spinning disk confocal imaging was performed with a Quorum spinning disk confocal head on a Zeiss Axiovert 200M microscope, equipped with a 63× NA 1.4 oil objective and 25× NA 0.8 multi-immersion objective, a 1.5× tube lens, and a back-thinned EM-CCD camera (C9100–13, Hamamatsu). Acquisition settings were controlled using Volocity software and were acquired at equal laser and exposure settings within experiments across compared conditions. Lightning microscopy was performed on a Leica SP8 Lightning Confocal DMI6000 microscope utilizing a 100× 1.4 NA (O, STED) objective and two HyD detectors. Acquisitions were driven by a Leica motorized XY stage and Leica SuperZ Galvo with Adaptive Focus Control. Acquisition settings were controlled using the Leica LAS, Lightning Module software, with matched settings within experiments.
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