Era antibody

Manufactured by Santa Cruz Biotechnology

The ERa antibody is a laboratory reagent used to detect the presence and localization of the estrogen receptor alpha (ERa) protein in biological samples. It is a specific and highly sensitive tool for researchers studying the role of ERa in various cellular processes and disease states.

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Lab products found in correlation

Phospho p44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions) Fgfr1 antibody, by Abcam (1 mentions) Phospho frs2, by Cell Signaling Technology (1 mentions)

2 protocols using era antibody

FGF2-Induced Signaling Pathway Analysis

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Cells were plated in DMEM/10% FBS supplemented with 2 ng/mL FGF2 and treated for 6 h with inhibitors or vehicle. Lysates were generated as previously described²⁸. For immunoblot analysis, the following antibodies were used: phospho-FRS2, phospho-p44/42 MAPK (Erk1/ 2), total p44/p42 MAPK (Erk½), and actin (Cell Signaling Technologies); FGFR1 antibody was obtained from Abcam; ERa antibody was obtained from SantaCruz. Immunoreactive proteins were visualized by enhanced chemiluminescence (Pierce).

Drago J., Formisano L., Juric D., Niemierko A., Servetto A., Wander S., Spring L., Vidula N., Peppercorn J., Younger J., Malvarosa G., Yuen M., Sgroi D., Isakoff S.J., Moy B., Ellisen L.W., Iafrate J., Arteaga C.L, & Bardia A. (2019). FGFR1 gene amplification mediates endocrine resistance but retains TORC sensitivity in metastatic hormone receptor positive (HR+) breast cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(21), 6443-6451.

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Carmine Staining of Whole Mounts

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All reagents used for carmine staining of whole mounts, including ethanol, xylene, and glacial acetic acid, were purchased from Fisher Scientific (Hampton, NH) unless stated otherwise. The NSD3 antibody used for western blotting and immunostaining was purchased from ProteinTech Group Inc. (Cat. No. 11345-1-AP) and the ERa antibody was purchased from Santa Cruz (Cat. No. sc-7207). Primers used for genotyping were purchased from IDT.

Turner-Ivey B., Smith E.L., Rutkovsky A.C., Spruill L.S., Mills J.N, & Ethier S.P. (2017). Development of mammary hyperplasia, dysplasia, and invasive ductal carcinoma in transgenic mice expressing the 8p11 amplicon oncogene NSD3. Breast cancer research and treatment, 164(2), 349-358.

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