The tdTomato DNA sequence, fused to the human CD63 gene (Entrez gene ID 967), was synthesized by GenScript Biotech Inc., and cloned into the pLenti-puro vector (Addgene, #39481) to create the pLenti-tdTomato-CD63 vector. Lentivirus particles of pLenti-tdTomato-CD63 were generated by transfection of HEK293FT cells with a transducing vector and the packaging vectors pLP1, pLP2, and pLP/VSV-G as previously described (Holehonnur et al., 2015 (link)). The supernatant containing virus particles was collected 48 and 72 h after transfection, filtered through a 0.45 μm Millex-HV filter (Millipore Sigma, SLHV033RS), concentrated by ultracentrifugation at 100,000 × g for 2 h, and stored at −80°C until use.
Entrez gene id 967
Manufactured by GenScript
Entrez gene ID 967 corresponds to the gene encoding the protein Adenylosuccinate synthetase. This enzyme catalyzes the first committed step in the de novo biosynthesis of adenosine monophosphate (AMP) from inosine monophosphate (IMP). It plays a crucial role in the purine nucleotide synthesis pathway.
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Lab products found in correlation
2 protocols using entrez gene id 967
1
Lentiviral Vector for tdTomato-CD63 Expression
2
Lentiviral Vector for tdTomato-CD63 Expression
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The tdTomato DNA sequence, fused to the human CD63 gene (Entrez gene ID 967), was synthesized by GenScript Biotech Inc., and cloned into the pLenti-puro vector (Addgene, #39481) to create the pLenti-tdTomato-CD63 vector. Lentivirus particles of pLenti-tdTomato-CD63 were generated by transfection of HEK293FT cells with a transducing vector and the packaging vectors pLP1, pLP2, and pLP/VSV-G as previously described (Holehonnur et al., 2015 (link)). The supernatant containing virus particles was collected 48 and 72 h after transfection, filtered through a 0.45 μm Millex-HV filter (Millipore Sigma, SLHV033RS), concentrated by ultracentrifugation at 100,000 × g for 2 h, and stored at −80°C until use.
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