Pm150810

In order to clarify the effect of circPOLR1C on the biological function of EC cells, we purchased human esophageal normal cell line Het-1A (ATCC® CRL-2692™, American Type Culture Collection) and various EC cell lines (KYSE-30, CL-0577, Procell, China; KYSE150, CL-0638, Procell, China; KYSE-220, laboratory preserved; TE-9, laboratory preserved; and EC9706, MZ-1077, MingZhouBio, China) and carried out differential expression research. According to the instructions of the manufacturer, cells were cultured with their specific complete cell culture media as follows: Het-1A cells were cultured in BEGM™ bronchial epithelial cell growth medium (CC-3171, Lonza/Clonetics Corporation, Switzerland) containing fetal bovine serum (FBS, 10%, SFBS, Bovogen, Australia); KYSE-30, KYSE150, KYSE-220, and TE-9 cells were maintained in RPMI-1640 medium (PM150110, Procell, China) supplemented with Ham's F-12 nutrient mixture (PM150810, Procell, China), FBS (10%), and penicillin-streptomycin solution (P/S, 1%, PB180120, Procell, China); EC9706 cells were grown in high-sugar Dulbecco's modified Eagle's medium (DMEM, C11995500BT, Gibco, USA) blended with FBS (10%) and P/S (1%). All cells were cultivated in a 5% MCO-20AIC CO₂ incubator (SANYO, Japan) at 37°C.

Human normal gastric mucosal cell line GES-1 and GC cell line AGS, HGC27, SNU-5, and MKN-45 were bought from Procell (CL-0563, CL-0022, CL-0107, CL-0444, and CL-0292, Wuhan, China). GES-1 and MKN-45 cells were cultured in RPMI-1640 medium (PM150110, Procell, China) enriched with 10% fetal bovine serum (FBS; 164210, Procell, China) and 1% penicillin-streptomycin solution (P/S; PB180120, Procell, China). AGS cells were incubated in Ham's F-12 Nutrient Mixture (PM150810, Procell, China) with 10% FBS and 1% P/S; HGC27 cells were cultured in RPMI-1640 medium enriched with 20% FBS and 1% P/S; SNU-5 cells were incubated in Iscove's modified Dulbecco medium (IMDM; PM150510, Procell, China) containing 20% FBS and 1% P/S. All cells were cultured at 37°C with 5% CO₂.