Hrp conjugated donkey anti goat

Manufactured by Jackson ImmunoResearch

HRP-conjugated donkey anti-goat is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to goat primary antibodies in immunoassays and other immunochemical applications.

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Lab products found in correlation

Irdye 800cw streptavidin, by LI COR (2 mentions) Hrp conjugated donkey anti mouse, by Jackson ImmunoResearch (2 mentions) Hrp conjugated donkey anti rabbit, by Jackson ImmunoResearch (2 mentions) Tetramethylbenzidine dihydrochloride, by Merck Group (1 mentions) Maxisorp 96 well elisa plate, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Donkey anti-goat HRP (12 citations) HRP-conjugated donkey anti-goat IgG (12 citations) Donkey anti-goat-HRP conjugated antibody (6 citations) HRP-conjugated donkey anti-goat IgG antibody (3 citations) HRP-conjugated goat anti-rabbit IgG and donkey anti-mouse IgG (2 citations)

3 protocols using hrp conjugated donkey anti goat

Western Blot Antibody Validation Panel

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Rabbit monoclonal anti-Cav1 (clone D46G3, Cell Signaling 3267, 1:1000), Mouse monoclonal anti-Ephx1 (clone 17, Santa Cruz sc-135984, 1:200), Rabbit polyclonal anti-Gpr107 (Proteintech 25076–1-AP, 1:600), Goat polyclonal anti-Gas1 (Novus AF2644, 1:1000), Rabbit polyclonal anti-Reep5 (Proteintech 14643–1-AP, 1:5000), Rabbit polyclonal anti-Tmem199 (Sigma-Aldrich HPA027051, 1:1000), Rabbit polyclonal anti-Tmem97 (Proteintech 26444–1-AP, 1:500), Rabbit polyclonal anti-VDAC (Cell Signaling 4866, 1:1000),Mouse monoclonal anti-Na+/K+-ATPase α1 (clone 464.6, Novus NB300–146, 1:5000), Mouse monoclonal anti-β-Actin (clone C4, Santa Cruz sc-47778, 1:750), IRDye 800CW Streptavidin (Licor 926–32230, 1:10000), Rabbit polyclonal anti-NPC1 (Novus NB400–148, 1:1500), Mouse monoclonal anti-c-Myc (clone 9E10, Sigma-Aldrich M4439, 1:2500), Mouse monoclonal anti-Flag M2 (clone M2, Sigma-Aldrich F1804, 1:5000), Mouse monoclonal anti-FLAG (clone H-5, Santa Cruz sc-166355, 1:1000), HRP-conjugated donkey anti-mouse (Jackson ImmunoResearch 715-035-151, 1:10000), HRP-conjugated donkey anti-rabbit (Jackson ImmunoResearch 715-035-152, 1:10000), HRP-conjugated donkey anti-goat (Jackson ImmunoResearch 715-035-003, 1:10000)

Cheng Y.S., Zhang T., Ma X., Pratuangtham S., Zhang G.C., Ondrus A.A., Mafi A., Lomenick B., Jones J.J, & Ondrus A.E. (2021). A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes. Nature chemical biology, 17(12), 1271-1280.

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Quantification of Complement Proteins

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MaxiSorp 96-well ELISA plates (Nalge Nunc International, Penfield, NY) were coated with 100 µl of diluted serum (1∶32,000 for C3; 1∶16,000 for C3a; 1∶6000 for C4), or with dilutions of purified C3, C3a or C4 human standards (all from Complement Technology). Plates were incubated at 4°C overnight, washed three times with PBS/Tween (0.2%), and wells were blocked with 200 µl of PBS containing 5% milk for 1 hr at 37°C. 100 µl of diluted primary goat anti-human C3 (1∶2000), rabbit anti-human C3a (1∶5000) or goat anti-human C4 (1∶2000) (all from Calbiochem) was added to each well and incubated for 1 h at room temperature (RT). Wells were washed five times with PBS/Tween before incubation at RT for 1 h with 100 µl per well of HRP-conjugated donkey anti-goat (1∶20,000 for C3 and C4) or goat anti-rabbit IgG (1∶50,000 for C3a) (Jackson ImmunoResearch Laboratories, PA) followed by development with substrate TMB (tetramethylbenzidine dihydrochloride, Sigma). The absorbance was determined at 450 nm on a ELx800 plate Absorbance Microplate Reader (Bio Tek Instruments, Inc., Vermont).

Mayer A.E, & Parks G.D. (2014). An AGM Model for Changes in Complement during Pregnancy: Neutralization of Influenza Virus by Serum Is Diminished in Late Third Trimester. PLoS ONE, 9(11), e112749.

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Western Blot Antibody Validation Panel

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