Inosine triphosphate

Manufactured by Merck Group

Sourced in Italy

Inosine triphosphate is a nucleotide compound found in living cells. It serves as a substrate for various enzymatic reactions, particularly those involved in energy metabolism and nucleic acid synthesis.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Inosine (74 citations)

6 protocols using inosine triphosphate

Poly(U) Polymerase Assay for RNA Tailing

Check if the same lab product or an alternative is used in the 5 most similar protocols

To test for tailing ³²P-labeled A24 as a substrate using residues (ATP, CTP, GTP, UTP, or ITP) a reaction containing labeled A24 RNA, 0.5 mM NTP, 50 mM NaCl, 10 mM MgCl₂, 10 mM Tris-Cl pH 7.9, 1 mM DTT, 500 µg/mL Bovine Serum Albumin (BSA), and 0.6 units of NEB poly(U) polymerase in a final volume of 5 µL was incubated at 37°C for 30 min. The standard reaction for adding homopolymers to the 3′ends of model mRNAs and yeast RNA preparations using Cid1 poly(U) polymerase is as follows: RNA (various amounts) in 0.1 mM EDTA in volumes up to 2.95 µL is denatured at 95°C for 2 min then placed on ice for 2 min. The RNA is added to a reaction containing 4mM NTP (ATP, CTP, GTP, UTP, or ITP) 50 mM NaCl, 13.5 mM MgCl₂, 10 mM Tris-Cl pH 7.9, 1 mM DTT, 500 µg/mL BSA, and 0.6 units of NEB poly(U) polymerase in a final volume of 7.5 µL and incubated at 37°C for 1 h. To test for incorporation of modified U residues, 200 fmol MYL6(A+) RNA was incubated with 1 mM concentration of modified UTP analog or a combination of 0.5 mM modified UTP analog and 0.5 mM UTP following the standard poly(U) polymerase reaction. Inosine triphosphate was purchased from Sigma. Modified UTP analogs were purchased from TriLink, Inc.

Vo J.M., Mulroney L., Quick-Cleveland J., Jain M., Akeson M, & Ares M J.r. (2021). Synthesis of modified nucleotide polymers by the poly(U) polymerase Cid1: application to direct RNA sequencing on nanopores. RNA, 27(12), 1497-1511.

+ Open protocol

+ Expand

Vascular Reactivity Regulation Mechanisms

Check if the same lab product or an alternative is used in the 5 most similar protocols

NaCl, KCl, MgCl₂, KH₂PO₄,CaCl₂, NaHCO₃, glucose, NaHS, l‐cysteine, phenylephrine, ACh, 5‐HT, l‐NIO (N⁵‐(1‐Iminoethyl)‐L‐ornithine dihydrochloride), Tris‐HCl, sodium deoxycholate, sodium dodecyl sulfate, EDTA, Igepal, protease inhibitor cocktail, BSA, Tween 20, β‐actin, methanol, ammonium acetate, acetic acid, creatine phosphokinase, creatine phosphate DEA‐NO, zinc acetate and, inosine triphosphate and stock solution of cGMP, cAMP and cIMP were supplied by Sigma‐Aldrich, (Milan, Italy). Inosine 3′‐5′‐cyclic monophoshate sodium salt was supplied by (Merck, Germany). Anti‐p‐PDE4A and anti‐p‐PDE5 were supplied by Fabgennix (Frisco, TX, USA). Anti‐PDE4A was supplied by ProteinTech (Manchester, UK). Anti‐PDE5 was supplied by Santa Cruz Biotechnology (Heidelberg, Germany). Anti‐eNOS was supplied by BD Transduction Laboratories (USA). Anti‐β‐actin was supplied by Merck (Germany). ODQ, U46619, FR 139317 and BQ788 were supplied by Tocris (Bristol, UK).

Mitidieri E., Vellecco V., Brancaleone V., Vanacore D., Manzo O.L., Martin E., Sharina I., Krutsenko Y., Monti M.C., Morretta E., Papapetropoulos A., Caliendo G., Frecentese F., Cirino G., Sorrentino R., d'Emmanuele di Villa Bianca R, & Bucci M. (2021). Involvement of 3′,5′‐cyclic inosine monophosphate in cystathionine γ‐lyase‐dependent regulation of the vascular tone. British Journal of Pharmacology, 178(18), 3765-3782.

+ Open protocol

+ Expand

Quantitative Analysis of Nucleotides

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adenosine, Adenosine Monophosphate (AMP), Adenosine triphosphate (ATP), Guanosine, Guanosine monophosphate (GMP), guanosine triphosphate (GTP), Inosine, Inosine monophosphate (IMP), and Inosine triphosphate (ITP) were purchased from Sigma Aldrich, St. Louis, MO; isotopic internal standards for each, (Ribose ¹³C₅) were from Cambridge Isotope Laboratories, Andover, MA.
Analytical grade reagents were purchased from Fisher Scientific, Fairlawn, NJ, (acetonitrile, methanol, formic acid, potassium chloride, phosphoric acid, and ammonium hydroxide). Ammonium acetate 5 M solution was purchased from Ambion^® and alkaline phosphatase was purchased from Sigma Aldrich, St. Louis. Ultrapure (UP) water was prepared in house from deionized water with a Barnstead Nanopure System (Thermo Fisher Scientific, Waltham, MA). Other supplies included Waters Sep-Pak Accell Plus QMA Cartridge, 3 cc (500 mg) (Waters Corporation, Milford, MA) and Varian Bond-Elut LRC Phenylboronic Acid (PBA) Cartridge 100mg/10 mL (Agilent, Santa Clara, CA). PAXgene^® tubes were from Qiagen, Valencia, CA.

Jimmerson L.C., Clayton C.W., MaWhinney S., Meissner E.G., Sims Z., Kottilil S, & Kiser J.J. (2016). Effects of ribavirin/sofosbuvir treatment and ITPA phenotype on endogenous purines. Antiviral research, 138, 79-85.

+ Open protocol

+ Expand

Quantitative Analysis of Nucleotides

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adenosine, Adenosine Monophosphate (AMP), Adenosine triphosphate (ATP), Guanosine, Guanosine monophosphate (GMP), guanosine triphosphate (GTP), Inosine, Inosine monophosphate (IMP), and Inosine triphosphate (ITP) were purchased from Sigma Aldrich, St. Louis, MO; isotopic internal standards for each, (Ribose ¹³C₅) were from Cambridge Isotope Laboratories, Andover, MA.
Analytical grade reagents were purchased from Fisher Scientific, Fairlawn, NJ, (acetonitrile, methanol, formic acid, potassium chloride, phosphoric acid, and ammonium hydroxide) as well as Whatman^® 903 DBS cards, bags and desiccant for DBS preparation and storage. Ammonium acetate 5M solution was purchased from Ambion® and alkaline phosphatase was purchased from Sigma Aldrich, St. Louis. Ultrapure (UP) water was prepared in house from deionized water with a Barnstead Nanopure System (Thermo Fisher Scientific, Waltham, MA). Other supplies included Waters Sep-Pak Accell Plus QMA Cartridge, 3cc (500mg) (Waters Corporation, Milford, MA) and Varian Bond-Elut LRC Phenylboronic Acid (PBA) Cartridge 100mg/10mL (Agilent, Santa Clara, CA). Blood products for lysed cellular matrix were obtained from Bonfils, Denver CO. Cell preparation tubes (CPT) and dipotassium EDTA tubes were purchased from Becton, Dickinson and Company, Franklin Lakes, NJ.

Jimmerson L.C., Bushman L.R., Ray M.L., Anderson P.L, & Kiser J.J. (2016). A LC-MS/MS method for quantifying adenosine, guanosine and inosine nucleotides in human cells. Pharmaceutical research, 34(1), 73-83.

+ Open protocol

+ Expand

Redox Reactions and Nucleotides Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hexaammineruthenium(III) chloride (Ru(NH3)6 2+/3+ ), potassium ferricyanide (Fe(CN)6 3-/4-), ammonium iron(II) sulfate hexahydrate (Fe 2+/3+ ), uridylic acid (UMP) and inosine triphosphate (ITP) were purchased from Sigma-Aldrich.

Takemoto M., Kamata T., Kato D, & Hara M. (2020). Controlling Surface Oxygen Concentration of a Nanocarbon Film Electrode for Improvement of Target Analytes. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36(4).

+ Open protocol

+ Expand

Tailing A24 RNA with Poly(U) Polymerase

Check if the same lab product or an alternative is used in the 5 most similar protocols

To test for tailing 32 P-labeled A24 as a substrate using residues (ATP, CTP, GTP, UTP or ITP) a reaction containing labeled A24 RNA, 0.5mM NTP, 50mM NaCl, 10mM MgCl 2 , 10 mM Tris-Cl pH 7.9, 1mM DTT, 500 ug/ml Bovine Serum Albumin (BSA), and 0.6 units of NEB poly(U) polymerase in a final volume of 5ul was incubated at 37°C for 30 minutes. The standard reaction for adding homopolymers to the 3′ends of model mRNAs and yeast RNA preparations using Cid1 poly(U) polymerase is as follows: RNA (various amounts) in 0.1mM EDTA in volumes up to 2.95uL is denatured at 95°C for 2 minutes then placed on ice for 2 minutes. The RNA is added to a reaction containing 4mM NTP (ATP, CTP, GTP, UTP or ITP) 50mM NaCl, 13.5mM MgCl 2 , 10 mM Tris-Cl pH 7.9, 1mM DTT, 500 ug/ml BSA, and 0.6 units of NEB poly(U) polymerase in a final volume of 7.5ul and incubated at 37°C for 1 hour. To test for incorporation of modified U residues, 200fmol MYL6(A+) RNA was incubated with 1mM concentration of modified UTP analog or a combination of 0.5mM modified UTP analog and 0.5mM UTP following the standard poly(U) polymerase reaction.
Inosine triphosphate was purchased from Sigma. Modified UTP analogs were purchased from TriLink, Inc.

Vo J., Mulroney L., Quick-Cleveland J., Jain M., Akeson M., & Ares M. (2021). Synthesis of modified nucleotide polymers by the poly(U) polymerase Cid1: Application to direct RNA sequencing on nanopores.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!