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2 protocols using anti ccna1

1

Antibody Panel for Cellular Signaling Analysis

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Antibodies used in this study include anti-β-catenin (BD Biosciences, Cat# 610153, RRID: AB_397554), anti-KI67 (Abcam, Cat# ab15580, RRID: AB_443209), anti-YAP (Cell Signaling Technology, Cat# 14074, RRID: AB_2650491), anti-CTGF (Sangon Biotech, Cat# D260212, Shanghai, China), anti-CYR61 (Cell Signaling Technology, Cat# 39382, RRID: AB_2799154), anti-ANKRD1 (Sangon Biotech, Cat# D121628, RRID: AB_2819216), anti-CCNA1 (Sangon biotech, Cat# D220507, RRID: AB_2819214), anti-CCND1 (Sangon biotech, Cat# D220509), anti-CCNE1 (Sangon biotech, Cat# D151593), anti-p-YAP Ser127 (Cell Signaling Technology, Cat# 13008, RRID: AB_2650553), anti-β-actin (Cell Signaling Technology, Cat# 8457, RRID: AB_10950489), anti-lamin B (Cell Signaling Technology, Cat# 13435, RRID: AB_2737428), anti-c-Myc (Cell Signaling Technology, Cat# 9402, RRID: AB_2151827), anti-CTNNB1 (Sangon biotech, Cat# D199519), anti-MET (Sangon biotech, Cat# D160981) and anti-FOXM1 (Proteintech, Cat# 13147-1-AP, RRID: AB_2106213, Rosemont, IL, USA).
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2

Signaling Pathway Antibody Panel

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Anti-YAP (Cat# 14074S, RRID: AB_2650491), anti-Phospho-YAP (Ser127) (Cat# 13008S, RRID: AB_2650553), anti-Cyclin D1 (Cat# 2922S, RRID: AB_2228523), anti-Cyclin D1 (Cat# 55506S, RRID: AB_2827374), anti-Cyclin E1 (Cat# 20808, RRID: AB_2783554), anti-β-actin (Cat# 4970S, RRID, AB_2223172), anti-Lamin B1 (Cat# 13435S, RRID: AB_2737428), anti-GAPDH (Cat# 2118S, RRID: AB_561053), anti-rabbit, IgG HRP-linked antibody (Cat# 7074S, RRID: AB_2099233), anti-mouse IgG, HRP-linked antibody (Cat# 7076S, RRID: AB_330924), signalstain® boost IHC detection reagent (HRP, rabbit) (Cat# 8114S, RRID: AB_10544930), signalstain® boost IHC detection reagent (HRP, mouse) (Cat# 8125S, RRID: AB_10547893) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-CCNA1 (Cat# D220507, RRID: AB_2819214), anti-CYR61 (Cat# D122190, RRID: AB_2819221), anti-ANKRD1 (Cat# D121628, RRID: AB_2819216) and anti-CTGF (Cat# D160212, RRID: AB_2819217) were purchased from Sangon Biotech (Shanghai, China). Anti-β-catenin (Cat# 610153, RRID: AB_397554) was purchased from BD Biosciences (Franklin Lakes, NJ, USA). Anti-caspase-3 (Cat# sc-56053, RRID: AB_781826) was purchased from Santa Cruz Biotechnology, Inc. (Carpinteria, CA, USA).
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