Quantiglo il 6 immunoassay

Manufactured by R&D Systems

Sourced in United States

The QuantiGlo IL-6 immunoassay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of interleukin-6 (IL-6) levels in cell culture supernates, serum, and plasma. The assay utilizes a microplate coated with a monoclonal antibody specific for IL-6 and a luminescent detection system.

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Lab products found in correlation

Quantikine scd14 immunoassay, by R&D Systems (2 mentions) Star automated coagulation analyzer, by Diagnostica Stago (1 mentions) Quantikine enzyme linked immunosorbent assay elisa kit, by R&D Systems (1 mentions) Spectramax absorbance reader, by Molecular Devices (1 mentions)

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QuantiGlo (10 citations)

3 protocols using quantiglo il 6 immunoassay

Biomarker Quantification in Epidemiological Study

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As is described elsewhere (28 (link), 34 (link)), blood samples were collected at one time point between 2005–2006 using serum separator and EDTA tubes and were shipped to a central repository at the Massachusetts Veterans Epidemiology Research and Information Center. Assays for biomarkers examined here were conducted at the Laboratory for Clinical Biochemistry Research at the University of Vermont and used four controls per sample to assess interassay coefficients of variability (CVs). sCD14 was quantified by an enzyme-linked immunosorbent assay (Quantikine sCD14 Immunoassay, R&D Systems, Minneapolis, MN) with a detectable range of 40–3,200 ng/ml. The interassay CVs ranged from 7.2–8.1%. IL-6 was quantified by a chemiluminescent immunoassay (QuantiGlo IL-6 immunoassay, R&D Systems, Minneapolis, MN) with detectable range of 0.4–10,000 pg/mL. The interassay CVs ranged from 7.7–12.3%. D-dimer was quantified by a STAR automated coagulation analyzer (Diagnostica Stago, Parsippany, NJ) using an immunoturbidometric assay (Liatest D-DI) with a detectable range of 0.01–20 ug/mL. The interassay CVs ranged from 2.8–14.8%. All three biomarkers were natural log transformed before analysis to approximate a normal distribution.

Stewart J.C., Polanka B.M., So-Armah K.A., White J.R., Gupta S.K., Kundu S., Chang C.C, & Freiberg M.S. (2020). Associations of Total, Cognitive/Affective, and Somatic Depressive Symptoms and Antidepressant Use with Cardiovascular Disease-Relevant Biomarkers in HIV: Veterans Aging Cohort Study. Psychosomatic medicine, 82(5), 461-470.

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Serum IL-6 and sCD14 Quantification

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Serum IL‐6 levels were measured in all subjects using a chemiluminescent immunoassay (QuantiGlo IL‐6 immunoassay, R&D Systems, Minneapolis, MN). Calibration was performed by the manufacturer and is traceable to National Institute for Biological Standards and Control 89/548 (IU/mL). Soluble CD14 (sCD14) was measured with an enzyme‐linked immunosorbent assay (Quantikine sCD14 Immunoassay, R&D Systems) with a detectable range of 40–3200 ng/mL, using a standard 200‐fold sample dilution. Statistical differences in cytokine levels between groups were assessed using two‐tailed t‐test, and across all four groups using one‐way analyses of variance (ANOVA) (GraphPad Software, La Jolla, CA).

Crothers K., Petrache I., Wongtrakool C., Lee P.J., Schnapp L.M, & Gharib S.A. (2016). Widespread activation of immunity and pro‐inflammatory programs in peripheral blood leukocytes of HIV‐infected patients with impaired lung gas exchange. Physiological Reports, 4(8), e12756.

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P2X7 Receptor Modulates Cytokine Release

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After washing, cells were incubated with P2X7 receptor agonist 2′(3′)-O-(4-Benzoylbenzoyl)adenosine-5′-triphosphate (BzATP) and supernatant was collected at the indicated times. IL-6 levels were determined using the QuantiGlo IL-6 Immunoassay or Quantikine enzyme-linked immunosorbent assay (ELISA) kits (both R&D Systems, Minneapolis, MN, USA) with signal detected using a luminometer (Thermo Fisher, Inc.) or SpectraMax Absorbance reader (Molecular Devices, San Jose, CA, USA), respectively. Parallel approaches were used to detect IL-1β levels using the QuantiGlo Human IL-1B/IL-1F2 Immunoassay (R&D Systems). Mg²⁺-free isotonic solution [(in mM) 105 NaCl, 5 KCl, 6 HEPES acid, 4 Na HEPES, 5 NaHCO₃, 60 mannitol, 5 glucose, and 1.3 CaCl₂] was used in some experiments as Mg²⁺ is reported to block the P2X7 receptor [23 (link)]. The absolute levels of IL-6 varied across experiments, perhaps due to small differences in extracellular volume or the freshness of the IL-6 solution used for standard curves.

Shao X., Guha S., Lu W., Campagno K.E., Beckel J.M., Mills J.A., Yang W, & Mitchell C.H. (2020). Polarized Cytokine Release Triggered by P2X7 Receptor from Retinal Pigmented Epithelial Cells Dependent on Calcium Influx. Cells, 9(12), 2537.

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