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Coomassie brilliant blue g 250 0.22

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Coomassie Brilliant Blue G-250 0.22% is a laboratory reagent used for protein quantification and detection in electrophoresis applications. It is a dye-based solution that binds to proteins, resulting in a color change that can be measured spectrophotometrically.

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Lab products found in correlation

Mini protean 3 cell, by Bio-Rad (2 mentions)

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Coomassie Brilliant Blue G-250 (31 citations) Coomassie Blue G-250 (10 citations) Coomassie Brilliant Blue (4 citations)

2 protocols using coomassie brilliant blue g 250 0.22

1

One-Dimensional Protein Separation by SDS-PAGE

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Protein separation was performed using one-dimensional sodium dodecyl sulfate electrophoresis on a 15% polyacrylamide gel under nonreducing conditions in the Mini-PROTEAN 3 Cell (Bio-Rad) [32 (link)]. Prior to electrophoresis, aliquots of the preparations were boiled for 3 minutes in lysis buffer (Tris-HCl 30 mM, pH 6.8; SDS 1%; urea 3 M; glycerin 10%; bromophenol blue 0.02%). Gels were stained with Coomassie Brilliant Blue G-250 0.22% (Serva).
Fedorova N.V., Ksenofontov A.L., Serebryakova M.V., Stadnichuk V.I., Gaponova T.V., Baratova L.A., Sud'ina G.F, & Galkina S.I. (2018). Neutrophils Release Metalloproteinases during Adhesion in the Presence of Insulin, but Cathepsin G in the Presence of Glucagon. Mediators of Inflammation, 2018, 1574928.
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2

SDS-PAGE for Protein Separation

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For protein separation, we used one-dimensional sodium dodecyl sulfate electrophoresis under nonreducing conditions on a 15% polyacrylamide gel in the Mini-PROTEAN 3 Cell (Bio-Rad, Berkeley, CA, USA). Before electrophoresis, aliquots of the samples were boiled for 3 min in lysis buffer (Tris-HCl 30 mM, pH 6.8; SDS 1%; urea 3 M; glycerin 10%; bromophenol blue 0.02%). Separated proteins were stained with Coomassie Brilliant Blue G-250 0.22% (Serva).
Galkina S.I., Fedorova N.V., Ksenofontov A.L., Serebryakova M.V., Golenkina E.A., Stadnichuk V.I., Baratova L.A, & Sud’ina G.F. (2021). Neutrophil Adhesion and the Release of the Free Amino Acid Hydroxylysine. Cells, 10(3), 563.
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