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Sureselect all exon v5 or v6

Manufactured by Agilent Technologies
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The SureSelect All Exon v5 or v6 is a targeted enrichment solution for whole exome sequencing. It allows for the capture and sequencing of the protein-coding regions of the human genome.

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Lab products found in correlation

Nextseq 500 device, by Illumina (4 mentions) Agilent sureselectxt reagent kit, by Agilent Technologies (3 mentions) Nextseq 500, by Illumina (1 mentions) Sureselectxt reagent kit, by Agilent Technologies (1 mentions)

Spelling variants of this product

SureSelect Human All Exon V5 or V6 kit (3 citations) SureSelect Human All Exon V5 or V6 (3 citations) SureSelect XT Human All Exon V5+UTR or V6+UTR Kit (2 citations) SureSelect XT Human All Exon v5 or v6 kit (2 citations)

5 protocols using sureselect all exon v5 or v6

1

Genomic DNA Exome Sequencing

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Two hundred nanogram of genomic DNA were used for library preparation by using the Agilent SureSelectXT reagent kit (Agilent Technologies, Santa Clara, CA, USA). The totality of the enriched library was used in the hybridization and captured with the SureSelect All Exon v5 or v6 (Agilent Technologies) baits. Following hybridization, the captured libraries were purified according to the manufacturer’s recommendations and amplified by polymerase chain reaction (12 cycles). Normalized libraries were pooled, and DNA was sequenced on an Illumina NextSeq500 device using 2 × 111-bp paired-end reads and multiplexed. More than 90% of the target sequence were covered with a read depth of at least 10X for somatic DNA.
Lecuelle J., Aarnink A., Tharin Z., Truntzer C, & Ghiringhelli F. (2021). Using Exome Sequencing to Improve Prediction of FOLFIRINOX First Efficacy for Pancreatic Adenocarcinoma. Cancers, 13(8), 1851.
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2

Exome Sequencing Library Preparation

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Two hundred nanograms of genomic DNA was used for library preparation, using the Agilent SureSelectXT Reagent Kit. The totality of the enriched library was used in the hybridization and captured with SureSelect All Exon v5 or v6 (Agilent) baits. After hybridization, the captured libraries were purified according to the manufacturer’s recommendations and amplified by polymerase chain reaction (12 cycles). Normalized libraries were pooled, and DNA was sequenced on an Illumina NextSeq 500 device using 2× 111-base pair (bp) paired-end reads and multiplexed.
Thibaudin M., Fumet J.D., Chibaudel B., Bennouna J., Borg C., Martin-Babau J., Cohen R., Fonck M., Taieb J., Limagne E., Blanc J., Ballot E., Hampe L., Bon M., Daumoine S., Peroz M., Mananet H., Derangère V., Boidot R., Michaud H.A., Laheurte C., Adotevi O., Bertaut A., Truntzer C, & Ghiringhelli F. (2023). First-line durvalumab and tremelimumab with chemotherapy in RAS-mutated metastatic colorectal cancer: a phase 1b/2 trial. Nature Medicine, 29(8), 2087-2098.
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3

Whole Exome Sequencing Protocol

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Two hundred ng of genomic DNA were used for library preparation, using the Agilent SureSelectXT reagent kit (Agilent Technologies, Santa Clara, CA, USA). The totality of the enriched library was used in the hybridization and captured with the SureSelect All Exon v5 or v6 (Agilent Technologies) baits. Following hybridization, the captured libraries were purified according to the manufacturer’s recommendations and amplified by polymerase chain reaction (12 cycles). Normalized libraries were pooled, and DNA was sequenced on an Illumina NextSeq500 device using 2 ×111-bp paired-end reads and multiplexed. More than 90% of the target sequence was covered with a read depth of at least 10X for somatic DNA (Supplementary Table 3).
Galland L., Ballot E., Mananet H., Boidot R., Lecuelle J., Albuisson J., Arnould L., Desmoulins I., Mayeur D., Kaderbhai C., Ilie S., Hennequin A., Bergeron A., Derangère V., Ghiringhelli F., Truntzer C, & Ladoire S. (2022). Efficacy of platinum-based chemotherapy in metastatic breast cancer and HRD biomarkers: utility of exome sequencing. NPJ Breast Cancer, 8, 28.
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4

Exome Sequencing of Tumor and Germline DNA

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Two hundred ng of genomic DNA were used for library preparation, using the Agilent SureSelectXT reagent kit (Agilent Technologies, Santa Clara, USA). The totality of enriched library was used in the hybridization and captured with the SureSelect All Exon v5 or v6 (Agilent Technologies) baits. Following hybridization, the captured libraries were purified according to the manufacturer's recommendations and amplified by polymerase chain reaction (12 cycles). Normalized libraries were pooled and DNA was sequenced on an Illumina NextSeq500 device using 2 × 111-bp paired-end reads and multiplexed. Tumor and germline DNA sequencing generated mean target coverages of 78X and 90X respectively, and a mean of more than 90% of the target sequence was covered with a read depth of at least 10X for somatic DNA.
Réda M., Richard C., Bertaut A., Niogret J., Collot T., Fumet J.D., Blanc J., Truntzer C., Desmoulins I., Ladoire S., Hennequin A., Favier L., Bengrine L., Vincent J., Hervieu A., Dusserre J.F., Lepage C., Foucher P., Borg C., Albuisson J., Arnould L., Nambot S., Faivre L., Boidot R, & Ghiringhelli F. (2020). Implementation and use of whole exome sequencing for metastatic solid cancer. EBioMedicine, 51, 102624.
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5

Exome Sequencing Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two hundred ng of genomic DNA was used for library preparation, using the Agilent SureSelectXT reagent kit (Agilent Technologies, Santa Clara, CA, USA). The totality of the enriched library was used in the hybridization and captured with the SureSelect All Exon v5 or v6 (Agilent Technologies) baits. Following hybridization, the captured libraries were purified according to the manufacturer’s recommendations and amplified by polymerase chain reaction (12 cycles). Normalized libraries were pooled, and DNA was sequenced on an Illumina NextSeq500 device using 2 × 111 bp paired-end reads and multiplexed. Tumor and germline DNA sequencing generated mean target coverages of 78× and 90×, respectively, and a mean of more than 90% of the target sequence was covered with a read depth of at least 10× for somatic DNA.
Dalens L., Lecuelle J., Favier L., Fraisse C., Lagrange A., Kaderbhai C., Boidot R., Chevrier S., Mananet H., Derangère V., Truntzer C, & Ghiringhelli F. (2023). Exome-Based Genomic Markers Could Improve Prediction of Checkpoint Inhibitor Efficacy Independently of Tumor Type. International Journal of Molecular Sciences, 24(8), 7592.
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