The powdered form of OS was purchased from a local supplier (Ethno Resources, Sungai Buloh, Selangor Malaysia) with a particle size of 40 meshes and stored at room temperature in a dark environment. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin strep (PS), and trypsin were purchased from Gibco, Life Technologies (Rockville, MD, USA). Ethanol, acetonitrile, methanol, formic acid, and dimethyl sulfoxide (DMSO) were purchased from QRec Asia (Selangor, Malaysia). 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2’-Azino-bis(3-Ethylbenzothiazoline-6-sulphonic acid) (ABTS), standard rosmarinic acid, eupatorin, sinensetin, and 3′-hydroxy-5,6,7,4′-tetramethoxyflavone were acquired from Sigma-Aldrich, Taufkirchen, Germany. Trolox and ascorbic acid were purchased from Merck, Darmstadt Germany.
2 2 diphenyl 1 picrylhydrazyl dpph 2 2 azino bis 3 ethylbenzothiazoline 6 sulphonic acid
Manufactured by Merck Group
Sourced in Germany, United States
2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical compound used as a reagent in antioxidant activity assays. 2,2'-Azino-bis(3-Ethylbenzothiazoline-6-sulphonic acid) is a chromogenic compound used in enzymatic activity and antioxidant capacity assays.
Automatically generated - may contain errors
Lab products found in correlation
Ascorbic acid, by Merck Group (2 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Trolox, by Merck Group (1 mentions)
Sinensetin, by Merck Group (1 mentions)
Eupatorin, by Merck Group (1 mentions)
3 hydroxy 5 6 7 4 tetramethoxyflavone, by Merck Group (1 mentions)
Phosphate buffer, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Butylated hydroxyl anisole, by Merck Group (1 mentions)
3 5 dinitrosalicylic acid, by Merck Group (1 mentions)
2 protocols using 2 2 diphenyl 1 picrylhydrazyl dpph 2 2 azino bis 3 ethylbenzothiazoline 6 sulphonic acid
1
Antioxidant Evaluation of Orthosiphon stamineus Extract
2
Antioxidant and Enzyme Inhibition Assays
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Butylated Hydroxyl anisole (BHA), ascorbic acid (AA), FeCl 2 , FeCl 3 , NaOH, potassium sodium tartrate, sodium carbonate, Tris-HCl buffer, phosphate buffer, 3,5-dinitrosalicylic acid, potassium persulfate, 2,2'-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3ethylbenzothiazoline-6-sulphonic acid) (ABTS) were all purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA). Other reagents used are 4-nitrophenyl-a-D-glucopyranoside, p-nitrophenyl palmitate, orlistat, methanol, hexane, ethyl acetate, and water. All chemicals used were of analytical standard.
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