Identification of constituents of ethylacetate partitioned fraction of A. digitata using HRGC-MS. The GC-MS analysis was performed using Agilent Technologies GC-MS (Model 7890A) equipped with Agilent 19091 S–433HP-5MS 5% phenyl methyl polysiloxane column (30 m × 250 μm x 0.25μm f.t). The analysis works on the principle that a mixture will separate into individual substances when heated. Pure helium gas was used as a carrier gas at a flow rate of 1.5 mL/min. GC-MS analysis resulting in chromatogram was compared to complete library using data base of National Institute of Standard and Technology (NIST).
Gc ms model 7890a
Manufactured by Agilent Technologies
The Agilent 7890A Gas Chromatograph coupled with a Mass Spectrometer (GC-MS) is an analytical instrument designed for the separation, identification, and quantification of chemical compounds. It combines the high-resolution separation capabilities of gas chromatography with the powerful identification and structural elucidation capabilities of mass spectrometry.
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2 protocols using gc ms model 7890a
1
Ethylacetate Fraction Chemical Analysis
2
GC/MS Analysis of A. comosus Extract
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A concentrated extract of A. comosus was dissolved in methanol and the solution was used for the GC/MS analysis. The analysis was performed using Agilent Technologies GC/MS (Model 7890A) equipped with Agilent 19091IS-433HP-5MS 5% Phenyl Methyl Silox column (30 m × 250 µm × film thickness 0.25 µm) coupled with mass spectrometry. Pure helium gas as carrier gas at 1.5 mL/min constant rate was used. The injector temperature was 250 ºC.
GC/MS analysis resulting in chromatogram was compared to the complete library using a data base of the National Institute of Standard and Technology (NIST). The values were presented as the relative percentage of the chemical components expressed as a percentage by peak area. The GC/MS profiling was performed at the Department of Chemical Engineering, University of Ilorin, Ilorin, Nigeria.
GC/MS analysis resulting in chromatogram was compared to the complete library using a data base of the National Institute of Standard and Technology (NIST). The values were presented as the relative percentage of the chemical components expressed as a percentage by peak area. The GC/MS profiling was performed at the Department of Chemical Engineering, University of Ilorin, Ilorin, Nigeria.
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