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Puromycin antibody

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Puromycin antibody is a lab equipment product used to detect the presence of puromycin, an antibiotic commonly used in cell culture experiments. The antibody can be used to identify cells that have been exposed to puromycin, which is often used as a selection marker.

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Lab products found in correlation

Rapamycin, by Merck Group (2 mentions) Pras40, by Cell Signaling Technology (2 mentions) Pan akt, by Cell Signaling Technology (2 mentions) Hygromycin b, by Corning (2 mentions) C flip antibody, by Enzo Life Sciences (2 mentions) Hexadecylsulfonylfluoride hdsf, by Santa Cruz Biotechnology (2 mentions) P s6 s235 6, by Cell Signaling Technology (2 mentions) β tubulin antibody, by Merck Group (2 mentions) Rag b, by Cell Signaling Technology (2 mentions) Tw 37, by Selleck Chemicals (2 mentions) C myc, by Santa Cruz Biotechnology (2 mentions) Mg132, by Merck Group (2 mentions) Cleaved parp, by Cell Signaling Technology (2 mentions) Bafilomycin a1, by Merck Group (2 mentions) Mcl 1, by Cell Signaling Technology (2 mentions) Puromycin, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions)

2 protocols using puromycin antibody

1

Comprehensive Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies recognizing pan-AKT, p-AKT (S473), GSK3β, p-GSK3β (S9), TSC2, p-TSC2 (T1462), S6K, p-S6K (T389), S6, p-S6 (S235/6), PRAS40, ULK1, Rag A, Rag B, Rag C, AMPK, cleaved PARP, and Mcl-1 were from Cell Signaling Technology. β-tubulin antibody was from Sigma-Aldrich. XPB (TFIIH p89 S-19), p53, c-Myc, and GFP antibodies were from Santa Cruz. puromycin antibody was from KeraFAST. REDD1/DDIT4 antibodies were from Novus and Bethyl. C-FLIP antibody was from Enzo Life Sciences. All antibodies were used at final concentrations of 1:1000 – 1:2000 except for cleaved PARP, c-Myc, p-TSC2, ULK1, and XPB (1:500), AMPK, P70S6K, and TSC2 (1:750), and β-tubulin, S6, and p-S6 (1:4000). DMSO, cycloheximide, puromycin, rapamycin, bafilomycin A1, and MG-132 were from Sigma-Aldrich. Hygromycin B was from Corning. Hexadecylsulfonylfluoride (HDSF) was from Santa Cruz. TW-37 was from Selleckchem. Torin1 was a kind gift from David Sabatini and Nathanael Gray. Smac mimetic was a kind gift from Xiaodong Wang.
Potts M.B., McMillan E.A., Rosales T.I., Kim H.S., Ou Y.H., Toombs J.E., Brekken R.A., Minden M.D., MacMillan J.B, & White M.A. (2015). Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B. Nature chemical biology, 11(6), 401-408.
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2

Comprehensive Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies recognizing pan-AKT, p-AKT (S473), GSK3β, p-GSK3β (S9), TSC2, p-TSC2 (T1462), S6K, p-S6K (T389), S6, p-S6 (S235/6), PRAS40, ULK1, Rag A, Rag B, Rag C, AMPK, cleaved PARP, and Mcl-1 were from Cell Signaling Technology. β-tubulin antibody was from Sigma-Aldrich. XPB (TFIIH p89 S-19), p53, c-Myc, and GFP antibodies were from Santa Cruz. puromycin antibody was from KeraFAST. REDD1/DDIT4 antibodies were from Novus and Bethyl. C-FLIP antibody was from Enzo Life Sciences. All antibodies were used at final concentrations of 1:1000 – 1:2000 except for cleaved PARP, c-Myc, p-TSC2, ULK1, and XPB (1:500), AMPK, P70S6K, and TSC2 (1:750), and β-tubulin, S6, and p-S6 (1:4000). DMSO, cycloheximide, puromycin, rapamycin, bafilomycin A1, and MG-132 were from Sigma-Aldrich. Hygromycin B was from Corning. Hexadecylsulfonylfluoride (HDSF) was from Santa Cruz. TW-37 was from Selleckchem. Torin1 was a kind gift from David Sabatini and Nathanael Gray. Smac mimetic was a kind gift from Xiaodong Wang.
Potts M.B., McMillan E.A., Rosales T.I., Kim H.S., Ou Y.H., Toombs J.E., Brekken R.A., Minden M.D., MacMillan J.B, & White M.A. (2015). Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B. Nature chemical biology, 11(6), 401-408.
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