Vero cells (# CCL-81), BHK-21 (# CCL-10), CHO-K1 (# CCL-61), CHO-pgsA-745 (# CRL-2242), CHO-pgsB-618 (# CRL-2241), CHO-pgsE-606 (# CRL-2246), HEK293-FT (# CRL-11268), Rhabdomyosarcoma (RD) cells (# CCL-136), and MRC-5 (# CCL-171) cells were from the American Type Culture Collection (ATCC). MonoMac-1 cells (# ACC 252) were from the DSMZ-German Collection of Microorganisms and Cell Cultures. Vero, BHK-21, MRC-5 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1, CHO-pgsA-745, CHO-pgsB-618, and CHO-pgsE-606 cells were grown in F-12K medium (Thermo Fisher # 21127022). MonoMac-1 cells were grown in RPMI 1640 (Thermo Fisher # 11875119). All cell media were supplemented with 8% (v/v) not heat inactivated FBS (Hyclone # SH30071.03). Cells were cultured at 37 °C with 5% CO2. Cells were passaged at ~80 to 90% confluence and seeded as detailed for each individual assays. MucilAir™ (Epithelix # EP02MP) and SmallAir™ (Epithelix # EP21SA) HAE reconstituted from human primary cells obtained from nasal or bronchial biopsies were maintained in air–liquid interphase with specific culture medium (Epithelix # EP04MM, # EP64SA), in Costar Transwell inserts (Corning, NY, USA) per the manufacturer’s instructions.
Ep04mm
Manufactured by Corning
Sourced in United States
The EP04MM is a lab equipment product from Corning. It serves as a core function in laboratory settings, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Cho k1, by American Type Culture Collection (2 mentions)
Mrc 5, by American Type Culture Collection (2 mentions)
Bhk 21, by American Type Culture Collection (2 mentions)
Cho pgse 606, by American Type Culture Collection (2 mentions)
Cho pgsb 618, by American Type Culture Collection (2 mentions)
Bhk 21, by Thermo Fisher Scientific (2 mentions)
Cho pgsa 745, by Thermo Fisher Scientific (2 mentions)
Cho pgsb 618, by Thermo Fisher Scientific (2 mentions)
Cho pgse 606, by Thermo Fisher Scientific (2 mentions)
Cho pgsa 745, by American Type Culture Collection (2 mentions)
F 12k medium, by Thermo Fisher Scientific (2 mentions)
Hek293ft, by American Type Culture Collection (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Vero cell, by American Type Culture Collection (2 mentions)
Costar transwell insert, by Corning (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Hek293ft, by Thermo Fisher Scientific (2 mentions)
Cho k1, by Thermo Fisher Scientific (2 mentions)
Mrc 5, by Thermo Fisher Scientific (2 mentions)
Monomac 1 cells, by Leibniz Institute DSMZ (2 mentions)
2 protocols using ep04mm
1
Characterization of Cell Lines and Airway Epithelia
2
Cell Culture Protocols for Diverse Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Vero cells (# CCL-81), BHK-21 (# CCL-10), CHO-K1 (# CCL-61), CHO-pgsA-745 (# CRL-2242), CHO-pgsB-618 (# CRL-2241), CHO-pgsE-606 (# CRL-2246), HEK293-FT (# CRL-11268), Rhabdomyosarcoma (RD) cells (# CCL-136), and MRC-5 (# CCL-171) cells were from the American Type Culture Collection (ATCC). MonoMac-1 cells (# ACC 252) were from the DSMZ-German Collection of Microorganisms and Cell Cultures. Vero, BHK-21, MRC-5 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1, CHO-pgsA-745, CHO-pgsB-618, and CHO-pgsE-606 cells were grown in F-12K medium (Thermo Fisher # 21127022). MonoMac-1 cells were grown in RPMI 1640 (Thermo Fisher # 11875119). All cell media were supplemented with 8% (v/v) not heat inactivated FBS (Hyclone # SH30071.03). Cells were cultured at 37° C with 5% CO2. Cells were passaged at ~80–90% confluence and seeded as detailed for each individual assays. MucilAir™ (Epithelix # EP02MP) and SmallAir™ (Epithelix # EP21SA) HAE reconstituted from human primary cells obtained from nasal or bronchial biopsies were maintained in air-liquid interphase with specific culture medium (Epithelix # EP04MM, # EP64SA), in Costar Transwell inserts (Corning, NY, USA) per the manufacturer’s instructions.
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