Samples (100 µL) were supplemented with 20 µL of 1-OHbenz[α]anthracene-13C6, (at 1 mg/mL) and 10 µL of B[α]P-d12 (at 1 mg/mL) as internal standards and adjusted to pH 5.7 with 200 µL of 1 M sodium acetate buffer. The hydrolysis, extraction and purification procedures were carried out in accordance with the analytical method recently described by Peiffer et al. [38] (link). Plasma extracts were reconstituted in 25 µL of MSTFA (N-methyl-N-(trimethylsilyl) trifluoroacetamide, Derivatization of target analytes was completed after 30 min at 60°C, and 2 µL of the extract were injected into the GC–MS/MS system. Analyses were carried out with an Agilent 7890A gas chromatograph equipped with a HP-5MS capillary column (30 m, 0.25 mm i.d., 0.25 µm film thickness), coupled with an Agilent 7000B triple quadrupole mass spectrometer operating in electron impact ionization mode and an Agilent CTC PAL autosampler. Analytical conditions used for chromatography and MS/MS detection were as previously described [39] (link). Calibration curves were performed using plasma specimens supplemented with increased concentration levels of B[α]P and of their metabolites from 0 to 125 ng/mL of plasma. Limits of quantification (LOQs) were evaluated at 0.25 ng/mL of plasma for B[α]P, ranged from 0.1 to 1.0 ng/mL for monohydroxylated- and dihydroxylated- forms of B[α]P.
7000b triple quadrupole mass spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The 7000B triple quadrupole mass spectrometer is a laboratory instrument designed for the analysis of chemical compounds. It utilizes triple quadrupole technology to perform high-sensitivity and high-selectivity mass spectrometric analyses.
Automatically generated - may contain errors
Lab products found in correlation
7890a gas chromatograph, by Agilent Technologies (2 mentions)
Hp 5ms capillary column, by Agilent Technologies (2 mentions)
Ctc pal autosampler, by Agilent Technologies (1 mentions)
Helix pomatia, by Merck Group (1 mentions)
7693 autosampler, by Agilent Technologies (1 mentions)
7890a gc system, by Agilent Technologies (1 mentions)
Hp 5ms ultra inert analytical column, by Agilent Technologies (1 mentions)
Masshunter software, by Agilent Technologies (1 mentions)
Agilent 7890a gas chromatograph, by Agilent Technologies (1 mentions)
Hp 5ms ultra inert column, by Agilent Technologies (1 mentions)
J w db 5ms, by Agilent Technologies (1 mentions)
Masshunter b 06, by Agilent Technologies (1 mentions)
Agilent 7890 gc, by Agilent Technologies (1 mentions)
5 protocols using 7000b triple quadrupole mass spectrometer
1
Quantification of Benzo[a]pyrene and Metabolites
2
Quantifying PAH metabolites in urine
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Fifty metabolites of PAH (mono-hydroxylated-PAH, OH-PAH), corresponding to the most common metabolized forms of the 16 PAH used for animal treatment, were assessed in urine samples using a previously published method25 (link). Briefly, urine samples were incubated overnight at 37 °C in present of sulfatase and glucuronidase from Helix pomatia (Sigma-Aldrich, Bormen, Belgium) at pH 5.6. Upon acidification with HCl 32%, the samples were further purified using successively a liquid-liquid extraction with ethyl acetate/cyclohexane (50:50, v/v) and a solid phase extraction onto an Envi-Chrom P cartridge (Sigma-Aldrich, Bormen, Belgium). OH-PAH were eluted with acetate/cyclohexane (50:50, v/v), evaporated, resuspended in cyclohexane/methanol/water (50:40:10, v/v/v). The methanol-water layer containing OH-PAH was evaporated until fully dried. The residue was then further derivatized with 20 µL MtBSTFA for 30 min at 60 °C and then 1 µL was injected into an Agilent 7890 A gas chromatograph equipped with an HP-5MS capillary column (30 m, 0.25 mm i.d., 0.25 µm film thickness), coupled with an Agilent 7000B triple quadrupole mass spectrometer operating in electron impact ionization mode26 (link).
3
Comprehensive GC-MS/MS Analysis of Pesticides
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A total of 71 GC-amenable pesticides were analyzed using an Agilent (Santa Clara, CA, USA) 7890A GC system equipped with an Agilent 7693 autosampler, interfaced to an Agilent 7000B triple quadrupole mass spectrometer. An Agilent HP-5MS Ultra Inert analytical column (30 × 0.25 mm, 0.25 μm) was used in the residue separation, with helium as a carrier gas at a constant flow rate of 1.25 mL min−1. The GC oven was operated under the following conditions: initial temperature of 75 °C held for 2.5 min, 50 °C min−1 rate to 150 °C, then 20 °C min−1 rate to 200 °C, and finally 16 °C min−1 rate to 310 °C and held for 15 min. The injection port temperature was 280 °C and 5 μL volume was injected with a multimode inlet in programmable temperature vaporizer (PTV) mode.
The triple quadrupole mass spectrometer was operated in electron ionization (EI) mode with an ionization voltage of 35 eV, ion source temperature of 230 °C, quadrupole temperature of 150 °C, and transfer line temperature of 300 °C, scanning from m/z 50 to 500 at 2.5 s per scan, solvent delay 3.75 min. Default instrument settings of collision gas flow of N2 at 1.5 mL min−1 and quench gas of He at 2.35 mL min−1 were used. Agilent MassHunter software was used for acquisition, data handling, and reporting.
The triple quadrupole mass spectrometer was operated in electron ionization (EI) mode with an ionization voltage of 35 eV, ion source temperature of 230 °C, quadrupole temperature of 150 °C, and transfer line temperature of 300 °C, scanning from m/z 50 to 500 at 2.5 s per scan, solvent delay 3.75 min. Default instrument settings of collision gas flow of N2 at 1.5 mL min−1 and quench gas of He at 2.35 mL min−1 were used. Agilent MassHunter software was used for acquisition, data handling, and reporting.
4
Multi-Residue Pesticide Analysis by GC-MS/MS
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Pesticides analyses were performed using Agilent 7890A gas chromatograph coupled with 7000B triple quadrupole mass spectrometer (Agilent Technologies, USA) using Mass Hunter software. The mass ionization was carried out using electron ionization mode at + 70 eV, with a sample injection volume of 1 µL. The temperatures of the transfer line and ion source are 280 ºC and 300 ºC, respectively. The analysis was carried out with a solvent delay of 2 m with applying multiple reaction monitoring (MRM) for the studied pesticides. Most MRM parameters for the studied pesticides (Table 1) were obtained from previous studies [28] with exchanging between the selected quantifiers and qualifiers for many pesticides. In addition, few pesticides have been optimized to get more sensitive and selective MRMs include biphenyl, penconazole, and ethion.
Chromatographic separations carried out using HP-5ms Ultra Inert column (30 m × 0.25 mm, 0.25 μm) which obtained from Agilent Technologies (USA). A pure Helium gas (>99.999 %) was used as carrier gas with a constant flow rate of 1.83 mL/m. The optimized oven temperature program and back flushing were used to shorten the analysis time with reducing the times of system maintenance. Backflush parameters were installed as follow; holding for 3 m; inlet pressure of 1 psi; three way splitter pressure of 40 psi; at oven temperature of 280 ºC.
Chromatographic separations carried out using HP-5ms Ultra Inert column (30 m × 0.25 mm, 0.25 μm) which obtained from Agilent Technologies (USA). A pure Helium gas (>99.999 %) was used as carrier gas with a constant flow rate of 1.83 mL/m. The optimized oven temperature program and back flushing were used to shorten the analysis time with reducing the times of system maintenance. Backflush parameters were installed as follow; holding for 3 m; inlet pressure of 1 psi; three way splitter pressure of 40 psi; at oven temperature of 280 ºC.
5
GC-MS Analysis of Complex Samples
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The GC-MS analyses were performed on an Agilent 7890 GC (Agilent Technologies, Palo Alto, CA, USA) with a multimode injector and a splitless liner containing a piece of glass wool. A fused silica high-temperature capillary column J&W DB-5MS (30 m × 0.25 mm i.d.; 0.25 μm film thickness) from Agilent was used at constant flow. The detector was an Agilent 7000B triple quadrupole mass spectrometer with inert electron ionization (EI) ion source. The mass spectrometer worked in selected ion monitoring (SIM) mode with EI ionization source at 70 eV. Helium with a purity of 99.9999% was used as carrier and quenching gas, and nitrogen with a purity of 99.999% as collision gas, both supplied by Air Liquide (Madrid, Spain).
For control purposes and data analysis, Agilent Mass Hunter B.06.00 software was used.
For control purposes and data analysis, Agilent Mass Hunter B.06.00 software was used.
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