Alexa fluor 568 goat anti rabbit

Manufactured by Abcam

Sourced in United States

Alexa Fluor® 568 Goat Anti-Rabbit is a secondary antibody conjugated with the Alexa Fluor® 568 fluorescent dye. It is designed to detect and visualize rabbit primary antibodies in immunological assays.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Anti cdh1, by Santa Cruz Biotechnology (2 mentions) Anti pin1, by Abcam (2 mentions) Alexa fluor 514 goat anti mouse, by Thermo Fisher Scientific (2 mentions) Las x software, by Leica (2 mentions) Alexa fluor 568 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Biotracker nir694, by Merck Group (1 mentions) Rabbit anti β 3 tubulin, by Abcam (1 mentions) Alexa fluor 488 goat anti rabbit, by Abcam (1 mentions) Alexa fluor 488 goat anti chicken, by Thermo Fisher Scientific (1 mentions) Chicken anti gfp, by Aves Labs (1 mentions) Anti citrullinated histone h3, by Abcam (1 mentions) Alexa fluor 488 goat anti rat, by Abcam (1 mentions) Anti ne, by Abcam (1 mentions)

Close spelling variants of this product

Alexa Fluor 568-conjugated goat anti-rabbit IgG Alexa Fluor 568 goat anti-rabbit IgG Alexa Fluor 568-conjugated goat anti-rabbit IgG antibody Goat anti-rabbit IgG H&L-Alexa Fluor 568 Alexa Fluor 568 Alexa 568 Rabbit anti-

4 protocols using alexa fluor 568 goat anti rabbit

Immunohistochemical Staining Antibodies

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The following antibodies were used for IHC studies: chicken anti-GFP (Aves Labs, reference GFP-1020, 1/1,000), mouse anti-Ecadh (BD Biosciences, USA, reference 610182, 1/300), rabbit anti-Krt19 (Abcam, UK, reference ab52625, 1/200), rabbit anti-βIII tubulin (Abcam, reference ab18207, 1/1,000), goat anti-chicken Alexa Fluor 488 (Thermo Fisher Scientific, USA, reference A-32931, 1/500), goat anti-mouse Alexa Fluor 568 (Thermo Fisher Scientific, reference A-11004, 1/500), goat anti-rabbit Alexa Fluor 568 (Abcam, reference ab175471, 1/500), goat anti-rabbit Alexa Fluor 488 (Abcam, reference ab11008, 1/500), and goat anti-rabbit Alexa Fluor 568 (Abcam, reference ab175471, 1/500). Nuclei were counterstained with Hoechst 33342 (Thermo Fisher Scientific, reference H3570, 1/2,000) and BioTracker NIR694 (Merck, USA, reference SCT118, 1/400).

Gautier B., Meneux L., Feret N., Audrain C., Hudecek L., Kuony A., Bourdon A., Le Guiner C., Blouin V., Delettre C, & Michon F. (2022). AAV2/9-mediated gene transfer into murine lacrimal gland leads to a long-term targeted tear film modification. Molecular Therapy. Methods & Clinical Development, 27, 1-16.

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Histopathological Analysis of Lung Tissue

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For histopathological analysis, the right lung lobes were fixed in 4% PFA and embedded in paraffin. Five-micron sections were placed onto glass slides and stained with hematoxylin and eosin (H&E) for microscopy analysis. To identify NET formation in lungs in vivo, 5 μm sections of paraffin-embedded mouse lungs were prepared and mounted on glass slides. After dewaxing, samples were permeabilized with 0.1% Triton X-100 for 10 min and blocked with PBS containing 1% BSA and 0.1% Tween-20. The sections were incubated with primary antibodies – anti-citrullinated-histone H3 (1:100; Abcam) and anti-NE (1:50; Abcam), followed by detection with Alexa Fluor 488 goat anti-rat (1:500; Abcam) and Alexa Fluor 568 goat anti-rabbit (1:500; Abcam) secondary antibodies for 1 hr at room temperature. Samples were also stained with DAPI for DNA detection.

Okeke E.B., Louttit C., Fry C., Najafabadi A.H., Han K., Nemzek J, & Moon J.J. (2020). Inhibition of neutrophil elastase prevents neutrophil extracellular trap formation and rescues mice from endotoxic shock. Biomaterials, 238, 119836.

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Immunostaining for PIN1 and CDH1 Colocalization

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Except where indicated otherwise the steps were performed at room temperature. Cells were rinsed with PBS twice and fixed with 2% PFA for 15 min. Fixative was removed by washing with PBS 3 times. Cells were then permeabilized with 0.1% Triton for 10 min. After removing Triton, cells were blocked with 5% BSA for 1 h and then incubated with anti-PIN1 (Abcam) and anti-CDH1 (Santa Cruz) antibodies overnight at 4 °C. After three washes with PBS, the cells were then incubated with Alexa Fluor® 514 Goat Anti-Mouse (Invitrogen) and Alexa Fluor® 568 Goat Anti-Rabbit (Abcam) antibodies for 1 h. Following the incubation, the cells were washed 3X with PBS and mounted for STED imaging. Colocalization rates were calculated using the LAS X software (Leica).

Ke S., Dang F., Wang L., Chen J.Y., Naik M.T., Li W., Thavamani A., Kim N., Naik N.M., Sui H., Tang W., Qiu C., Koikawa K., Batalini F., Stern Gatof E., Isaza D.A., Patel J.M., Wang X., Clohessy J.G., Heng Y.J., Lahav G., Liu Y., Gray N.S., Zhou X.Z., Wei W., Wulf G.M, & Lu K.P. (2024). Reciprocal antagonism of PIN1-APC/CCDH1 governs mitotic protein stability and cell cycle entry. Nature Communications, 15, 3220.

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Colocalization of PIN1 and CDH1 in Cells

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Except where indicated otherwise the steps were performed at room temperature. Cells were rinsed with PBS twice and fixed with 2% PFA for 15 min. Fixative was removed by washing with PBS 3 times. Cells were then permeabilized with 0.1% Triton for 10 min. After removing Triton, cells were blocked with 5% BSA for 1 hour and then incubated with anti-PIN1 (Abcam) and anti-CDH1 (Santa Cruz) antibodies overnight at 4°C. After three washes with PBS, the cells were then incubated with Alexa Fluor^® 514 Goat Anti-Mouse (Invitrogen) and Alexa Fluor^® 568 Goat Anti-Rabbit (Abcam) antibodies for 1 hour. Following the incubation, the cells were washed 3X with PBS and mounted for STED imaging. Colocalization rates were calculated using the LAS X software (Leica).

Ke S., Dang F., Wang L., Chen J.Y., Naik M.T., Thavamani A., Liu Y., Li W., Kim N., Naik N.M., Sui H., Tang W., Qiu C., Koikawa K., Batalini F., Wang X., Clohessy J.G., Heng Y.J., Lahav G., Gray N.S., Zho X.Z., Wei W., Wulf G.M, & Lu K.P. (2023). Reciprocal inhibition of PIN1 and APC/CCDH1 controls timely G1/S transition and creates therapeutic vulnerability. Research Square.

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