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Thunderbird probe qpcr master mix

Manufactured by Toyobo
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THUNDERBIRD Probe qPCR Master Mix is a ready-to-use solution for quantitative real-time PCR (qPCR) applications using probe-based detection methods. It contains all the necessary components, including DNA polymerase, buffer, and dNTPs, for efficient and accurate qPCR amplification.

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Lab products found in correlation

Cfx96 real time system, by Bio-Rad (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Taqman assay, by Thermo Fisher Scientific (1 mentions) Quantinova reverse transcription kit, by Qiagen (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Gdna remover kit, by Toyobo (1 mentions) Stepone real time system, by Thermo Fisher Scientific (1 mentions) Revetra ace qpcr master mix, by Toyobo (1 mentions)

Close spelling variants of this product

THUNDERBIRD Probe qPCR Mix (171 citations) THUNDERBIRD qPCR Mix (106 citations) QPCR Master Mix (4 citations) THUNDERBIRD Probe (3 citations)

2 protocols using thunderbird probe qpcr master mix

1

RNA Extraction and qRT-PCR Analysis

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Total RNAs were extracted with TRIZOL Reagents (Thermo Fisher Scientific) and reverse transcribed using QuantiNova Reverse Transcription Kit (Qiagen). Quantitative real-time RT-PCR were performed on a CFX96 Real-Time System (Bio-Rad) using Taqman Assays (Thermo Fisher Scientific) and THUNDERBIRD Probe qPCR Master Mix (TOYOBO). RPL7 was used as an internal control. Quantitative real-time RT-PCR was performed in triplicate for each sample.
Enomoto Y., Li P., Jenkins L.M., Anastasakis D., Lyons G.C., Hafner M, & Leonard W.J. (2021). Cytokine-enhanced cytolytic activity of exosomes from NK Cells. Cancer Gene Therapy, 29(6), 734-749.
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2

qPCR Gene Expression Analysis

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Isolated RNA was subjected to cDNA synthesis using ReveTraAce qPCR Master Mix with a gDNA Remover Kit (Toyobo). qPCR was performed using a StepOne Real‐Time System (Applied Biosystems/Thermo Fisher Scientific Inc.) with THUNDERBIRD Probe qPCR Master Mix (Toyobo Co., Ltd) and the indicated TaqMan probes (Applied Biosystems/Thermo Fisher Scientific, Inc.; Supporting Information File S1).
Hirai I., Funakoshi T., Kamijuku H., Fukuda K., Mori M., Sakurai M., Koda Y., Kato J., Mori T., Watanabe N., Noji S., Yaguchi T., Iwata T., Ohta S., Fujita T., Tanosaki R., Handa M., Okamoto S., Amagai M, & Kawakami Y. (2021). Adoptive cell therapy using tumor‐infiltrating lymphocytes for melanoma refractory to immune‐checkpoint inhibitors. Cancer Science, 112(8), 3163-3172.
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