CC patient and control tissues were collected from the Affiliated Kunshan Hospital of Jiangsu University. The patient tissues were preserved in liquid nitrogen and lysed using RIPA lysis buffer (Strong) (MedChemExpress, China). A BCA Protein Quantification Kit (Vazyme, China) was used to measure the protein concentration. Proteins were transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA) after separation through 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Then, the membranes were incubated with primary antibodies against the following: NUP107/SEC13/ALDH7A1/ALG1/CHPF/FAM162A/FBP2/GALK1/IDH1/TGFA/VLDLR/XYLT2/OGDHL (Abcam, USA, 1:1000) and GAPDH (Abcam, USA, 1:3000). The membranes were washed and incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1:5000; Sigma). The signals were visualized using BIO-RADXR.
Ripa lysis buffer strong
Manufactured by MedChemExpress
Sourced in China
RIPA lysis buffer (Strong) is a solution designed for the efficient extraction and solubilization of proteins from cellular samples. It is a robust and effective buffer that can disrupt a wide range of cell types, releasing their protein contents for further analysis.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Abcam (2 mentions)
Nitrocellulose membrane, by Merck Group (2 mentions)
Horseradish peroxidase labeled goat anti rabbit igg, by Merck Group (1 mentions)
Bca protein quantification kit, by Vazyme (1 mentions)
Bca protein quanti cation kit, by Vazyme (1 mentions)
Hrp labeled goat anti rabbit igg, by Merck Group (1 mentions)
2 protocols using ripa lysis buffer strong
1
Comparative Proteomics of Colorectal Cancer Tissues
2
Western Blot Analysis of Gene Expression
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Model gene validation by Western blot analysis CC patients' tissues and control were collected from A liated Kunshan Hospital of Jiangsu University. Patients' tissues were preserved in liquid nitrogen and lysed with RIPA Lysis Buffer (Strong) (MedChemExpress, China). BCA Protein Quanti cation Kit (Vazyme, China) was used to measure protein concentration. Proteins were transferred to nitrocellulose membrane (Millipore, USA) after separated by 10% SDS-PAGE. Then, incubated with primary antibodies: NUP107/SEC13/ALDH7A1/ALG1/CHPF/FAM162A/FBP2/GALK1/IDH1/TGFA/VLDLR/XYLT2/OGDHL (Abcam, USA, 1:1000), GAPDH (Abcam, USA, 1:3000). The membrane was washed and was incubated with HRP-labeled goat anti-rabbit IgG (1:5000, Sigma). Signals were visualized with BIO-RADXR.
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