Immunoblotting was performed as described previously.50 (link) Anti-BRD4 antibody (Bethyl Laboratories, Montgomery, AL, USA), anti-PD-L1 antibody (Abcam, Cambridge, UK), and anti-IDO1 antibody (Abcam, Cambridge, UK) were used in this study.
Anti ido1 antibody
Manufactured by Abcam
Sourced in United Kingdom
The Anti-IDO1 antibody is a research tool used for the detection and analysis of indoleamine 2,3-dioxygenase 1 (IDO1) in various biological samples. IDO1 is an enzyme that plays a role in the metabolism of the amino acid tryptophan. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to study the expression and distribution of IDO1 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Anti brd4 antibody, by Fortis Life Sciences (1 mentions)
Anti pd l1 antibody, by Abcam (1 mentions)
Anti mtor antibody, by Cell Signaling Technology (1 mentions)
Ecl kit, by Wuhan Servicebio Technology (1 mentions)
Anti ps6k1 antibody, by Cell Signaling Technology (1 mentions)
Anti s6k1 antibody, by Cell Signaling Technology (1 mentions)
Anti akt antibody, by Cell Signaling Technology (1 mentions)
Anti p mtor antibody, by Cell Signaling Technology (1 mentions)
Anti p akt antibody, by Cell Signaling Technology (1 mentions)
Ripa lysis buffer, by Merck Group (1 mentions)
Anti gapdh antibody, by Proteintech (1 mentions)
2 protocols using anti ido1 antibody
1
Immunoblotting of BRD4, PD-L1, and IDO1
2
Protein Expression Analysis by Western Blot
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Cultured cells or heart tissues were used to extract total protein with RIPA lysis buffer (Millipore) with protease inhibitor cocktail (Biomake). Next, standard Western blot was performed to determine the levels of interested proteins according to a protocol modified from previous studies [31 (link), 32 (link)]. The primary antibodies used for Western blot assay are :
Anti-IDO1 antibody (Abcam; 1:1000), anti-GAPDH antibody (Proteintech; 1:2000), anti-pAKT antibody (Cell Signaling Technology; 1:1000), anti-AKT antibody (Cell Signaling Technology; 1:1000), anti-pmTOR antibody (Cell Signaling Technology; 1:1000), anti-mTOR antibody (Cell Signaling Technology; 1:1000), anti-pS6K1 antibody (Cell Signaling Technology; 1:1000), anti-S6K1 antibody (Cell Signaling Technology; 1:1000). The secondary antibodies (1:5000) and ECL kit were purchased from Servicebio.
Anti-IDO1 antibody (Abcam; 1:1000), anti-GAPDH antibody (Proteintech; 1:2000), anti-pAKT antibody (Cell Signaling Technology; 1:1000), anti-AKT antibody (Cell Signaling Technology; 1:1000), anti-pmTOR antibody (Cell Signaling Technology; 1:1000), anti-mTOR antibody (Cell Signaling Technology; 1:1000), anti-pS6K1 antibody (Cell Signaling Technology; 1:1000), anti-S6K1 antibody (Cell Signaling Technology; 1:1000). The secondary antibodies (1:5000) and ECL kit were purchased from Servicebio.
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