The chemicals and solutions used for tissue fixation, histology, and immunohistochemistry were purchased from Applichem (acetone, catalogue number A1582; ethylenediaminetetra-acetic acid, catalogue number A3553), Carl Roth (Alizarin red S or Alizarin sulphonic acid sodium salt; catalogue number 0348.2; ethanol, catalogue number K9285; formaldehyde solution 37%, catalogue number 4979.1; glacial acetic acid, catalogue number 3738.4; hydrogen peroxide, catalogue number 9681.4; molybdatophosphoric acid hydrate, catalogue number 4440.3; nuclear fast red aluminum sulfate solution, catalogue number N069.1; Orange G, catalogue number 0318.2; Roticlear, catalogue number A538.1; Weigert's hematoxylin solution A and B, catalogue numbers X906.1 and X907.1; xylene, catalogue number 9713.5), Dako (antibody diluent, background reducing, S302283–2), Sigma-Aldrich (acid fuchsin, catalogue number F8129; citric acid monohydrate, catalogue number C1909; ponceau xylidine, catalogue number P2395; potassium hexacyanoferrate, catalogue number P3289; saturated picric acid solution, catalogue number P6744; and zinc formaline, catalogue number Z2902–3), Vector Laboratories (AEC Peroxidase Substrate Kit, catalogue number SK-4200; Vectastain Elite ABC-HRP kit, catalogue number PK-6100) or Waldeck GmbH (azophloxine, catalogue number 1B-103; light green SF, catalogue number 1B-211R).
Xylene
Manufactured by Agilent Technologies
Xylene is a clear, colorless, flammable, and aromatic organic solvent commonly used in various laboratory applications. It has a distinctive sweet odor and is derived from natural gas or petroleum. Xylene's primary function is as a versatile solvent, suitable for dissolving a wide range of organic compounds, including resins, oils, paints, and varnishes. It is often utilized in chemical synthesis, sample preparation, and instrumental analysis in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Acid fuchsin, by Merck Group (1 mentions)
Hydrogen peroxide, by Agilent Technologies (1 mentions)
Acetone, by AppliChem (1 mentions)
Ethylenediaminetetraacetic acid, by Carl Roth (1 mentions)
3 3 diaminobenzidine (dab), by Agilent Technologies (1 mentions)
Ab183105, by Abcam (1 mentions)
Anti rabbit anti mouse envision, by Agilent Technologies (1 mentions)
2 protocols using xylene
1
Tissue Fixation and Histological Staining Protocols
2
Immunohistochemical Analysis of TERT in RCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Representative tissue cores from formalin-fixed and paraffin-embedded RCC tumors were selected by an experienced pathologist and collected in a tissue microarray (TMA), as recently published [16] . Briefly, all TMA sections were deparaffinized at 60 °C for 20 minutes and dehydrated with graded alcohol. Endogenous peroxides and non-reactive staining were blocked with 3% H2O2 for 20 minutes at room temperature. After washing the tissue sections three times, antigen retrieval was performed by immersing the tissues in citrate buffer (pH =6.0) for 10 minutes in an autoclave. The tissue sections were incubated with primary antibody, anti-Telomerase reverse transcriptase antibody (ab183105, abcam, dilution: 1/500), overnight at 4°C. TMA slides were then incubated with anti-rabbit/anti-mouse Envision (Dako, Denmark) as a secondary antibody for 30 minutes. Staining patterns were visualized by exposure to 3, 3′-diaminobenzidine (DAB, Dako) followed by counterstaining with hematoxylin visualize antigen (Dako). Finally, the slides were dehydrated in alcohol, cleared in xylene (Dako), and mounted for examination. In each run of the experiment, human tonsillar tissue was used as a positive control, and for a negative control, the primary antibody was replaced with Tris-buffered saline.
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