Classic vortex mixer

Manufactured by VELP Scientifica

Sourced in Italy

The Classic Vortex Mixer is a laboratory instrument designed to mix and agitate samples. It utilizes a rotating motion to create a vortex within the sample container, effectively mixing the contents.

Automatically generated - may contain errors

Lab products found in correlation

U 2900 spectrophotometer, by Hitachi (2 mentions) Orion 9609bnwp, by Thermo Fisher Scientific (1 mentions) Orion ea 940, by Thermo Fisher Scientific (1 mentions) Rqflex10 reflectometer, by Merck Group (1 mentions) Reflectoquant, by Merck Group (1 mentions) Purelink microbiome dna purification kit, by Thermo Fisher Scientific (1 mentions) Qubit fluorometer, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Classic advanced vortex mixer Vortex mixer

5 protocols using classic vortex mixer

Emulsifying and Stability Indices of Aquafaba

Check if the same lab product or an alternative is used in the 5 most similar protocols

The emulsifying activity index (EAI) and the emulsion stability index (ESI) were determined according to the procedure described by Cheung et al. [31 (link)] but developed initially by Pearce and Kinsella [32 (link)]. In brief, 5.0 g of aquafaba or egg yolk was homogenized with 5.0 g of RRO using a homogenizer at a speed of 8000 rpm for 5 min. Then, a 50 µL aliquot of the emulsion was diluted to 7.5 mL of 0.1% sodium dodecyl sulphate (SDS) and vortexed using a classic vortex mixer (Velp Scientifica Srl, Usmate (MB), Italy) for 10 s. The absorbance of the diluted emulsion samples was measured at λ = 500 nm by a Hitachi U-2900 spectrophotometer (Tokyo, Japan). The EAI and ESI were calculated using the following equations:

EAI (\frac{m^{2}}{g}) = \frac{2 \cdot 2.303 \cdot A_{0} \cdot N}{c \cdot φ \cdot ϕ \cdot 10000}

ESI (\min) = \frac{A_{0}}{A_{0} - A_{10}} \cdot t

where, A₀ and A₁₀ are the absorbance values measured at an initial time, and after 10 min, respectively, t is the time interval (10 min), N is the dilution factor, c is the protein concentration (g/mL), φ is the oil volume fraction of the emulsion and ϕ is an optical path (1 cm).

Włodarczyk K., Zienkiewicz A, & Szydłowska-Czerniak A. (2022). Radical Scavenging Activity and Physicochemical Properties of Aquafaba-Based Mayonnaises and Their Functional Ingredients. Foods, 11(8), 1129.

+ Open protocol

+ Expand

DPPH Radical Scavenging Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

In brief, film samples (0.1 g) were ground in an electric laboratory mill (FW100; Chemland; Stargard Szczeciński, Poland). Then, 6 mL of DPPH solution was added to the test tube containing the film sample. In the next step, samples were shaken vigorously (Classic Vortex Mixer; Velp Scientifica Srl; Usmate (MB), Italy) for 10 min to facilitate the reaction with the reagent. After shaking, test tubes were put in the dark for 15 min. After this time the absorbance of the optically clear supernatant was measured spectrophotometrically at 517 nm using a Hitachi U-2900 spectrophotometer (Tokyo, Japan).

Tymczewska A., Furtado B.U., Nowaczyk J., Hrynkiewicz K, & Szydłowska-Czerniak A. (2022). Functional Properties of Gelatin/Polyvinyl Alcohol Films Containing Black Cumin Cake Extract and Zinc Oxide Nanoparticles Produced via Casting Technique. International Journal of Molecular Sciences, 23(5), 2734.

+ Open protocol

+ Expand

Fluoride Concentration Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluoride concentrations were measured using an ion-specific fluoride electrode (Orion 9609 BNWP, Thermo Fisher Scientific Inc., Waltham, MA, USA) coupled to an ion analyzer (Orion EA-940 Thermo Fisher Scientific Inc., Waltham, MA, USA). Before each reading, samples were shaken with a vibrator (Classic Vortex Mixer, Velp Scientifica, Italy) to homogenize the sample. The electrode was calibrated beforehand with standard solutions from 0.125 to 2.0 ppm F, mixing 1 mL of each standard solution with 1 mL of TISAB II (1.0 M pH acetate buffer 5.0; 1.0 M NaCl and 4% CDTA). Once calibrated, the samples were read, for which we mixed 1 mL of each saliva sample with 1 mL of TISAB II (Hanna Instruments, Woonsocket, RI, USA). The results in mV were converted into fluoride concentrations (ppm) using the standard calibration curves measured immediately before the analysis.

Poza-Pascual A., Serna-Muñoz C., Pérez-Silva A., Martínez-Beneyto Y., Cabello I, & Ortiz-Ruiz A.J. (2021). Effects of Fluoride and Calcium Phosphate-Based Varnishes in Children at High Risk of Tooth Decay: A Randomized Clinical Trial. International Journal of Environmental Research and Public Health, 18(19), 10049.

+ Open protocol

+ Expand

Saliva pH and Lactic Acid Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Saliva samples were thawed and shaken for 10 s at 20 °C (ClassicVortex Mixer, Velp Scientifica, Usmate Velate, Monza y Brianza, Italy) and 15 µL of the saliva sample was poured onto a pH test strip (range 4.0–9.0; Code. 1.16996.0001; Reflectoquant^™ Merck, Darmstadt, Germany) which was introduced in a RQflex^®10 reflectometer (Merck Millipore, Darmstadt, Germany) to provide the pH value. A volume of 30 µL of the saliva sample was poured onto a lactic acid test strip (range 1.0–60.0 mg/L; Code. 1.16127.0001; Reflectoquant^™ Merck, Darmstadt, Germany) which was introduced in a RQflex^® 10 reflectometer (Merck Millipore, Darmstadt, Germany) to provide the lactic acid value.

+ Open protocol

+ Expand

Stool DNA Extraction and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was isolated from a 0.2 g stool sample using a commercial kit (PureLink^TM Microbiome DNA Purification Kit) following the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) with little modifications. The use of standard bead-beater recommended by the manufacturer was replaced by simple laboratory benchtop vortexer (CLASSIC Vortex Mixer product code F202A0173, VELP Scientifica, Via Stazione 16-20865-Usmate Velate (MB), Italy). Bead-tubes containing the samples were fixed horizontally on the pad of bench top vortexer with the help of scotch tape at room temperature and vortexed at 2000 rpm for 8 min. To assure the NGS quality control (QC), all the extracted DNA samples were then quantified using Qubit fluorometer following manufacturer’s instructions (Qubit™ fluorometer, Invitrogen, Carlsbad, CA, USA) [29 (link)]. The quality of DNA samples were also checked using 1.0% agarose gel. Following quality control (DNA qualitative and quantitative analysis), all the DNA samples were then normalized to 0.2 ng/µL (1 ng/5µL).

Afridi O.K., Ali J, & Chang J.H. (2021). Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing. Microorganisms, 9(3), 616.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!