For immunostaining, the following primary antibodies were applied: polyclonal rabbit anti-DSG2 (#610121, Progen) and monoclonal mouse anti-N-cad (#610921, BD Transduction). As secondary antibodies, Cy2- and Cy3-conjugated goat anti-rabbit or goat anti-mouse secondary antibodies (Dianova, Hamburg, Germany), STAR red goat anti-rabbit IgG (Abberior GmbH, Göttingen, Germany, #2-0012-011-9) or Alexa Fluor 594 goat anti-Mouse IgG (Abcam, Berlin, Germany, #ab150116) for STED were applied. For western blots, monoclonal mouse anti-Dsg1/2 (#61002, Progen, monoclonal mouse anti-N-cad (#610921, BD Transduction), mouse monoclonal anti-PG (#61005. Progen) and monoclonal mouse anti-Tubulin (#ab7291) primary antibodies were used. Polyclonal goat anti-rabbit (#111-035-045, Jackson ImmunoResearch Europe Ltd.) and mouse (#115-035-068, Jackson ImmunoResearch Europe Ltd.) HRP conjugated antibodies were used as secondary bodies.
Yeruva S., Kempf E., Egu D.T., Flaswinkel H., Kugelmann D, & Waschke J. (2020). Adrenergic Signaling-Induced Ultrastructural Strengthening of Intercalated Discs via Plakoglobin Is Crucial for Positive Adhesiotropy in Murine Cardiomyocytes. Frontiers in Physiology, 11, 430.
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