The following antibodies were used for flow cytometric analysis: rat anti-CD4-PE-Cy7 (BD PharmingenTM, Allschwil, Switzerland, #552775; 1:100); rat anti-CD8-PE-Cy5 (BD PharmingenTM, Allschwil, Switzerland, #553034; 1:100); anti-CD19-PE-Cy7 (Biolegend, San Diego, CA, USA, #115520; 1:100); hamster anti-mouse anti-CD3-FITC (BD PharmingenTM, Allschwil, Switzerland, #561827; 1:100); rat anti-mouse anti-CD43-FITC (BD PharmingenTM, Allschwil, Switzerland, #561856; 1:100); rat anti-mouse anti-CD45R/B220-APC (BD PharmingenTM, Allschwil, Switzerland; #553092; 1:100); rat anti-mouse anti-IgM-PE-Cy7 (BD PharmingenTM, Allschwil, Switzerland, #552867; 1:100); rat anti-mouse IgG1-APC (BD PharmingenTM, Allschwil, Switzerland; #550874; 1:100). A LIVE/DEAD™ fixable violet dead cell stain kit (ThermoFisher Scientific, Waltham, MA, USA; #L34955; 1:1000) was used to identify dead cells.
Rat anti cd4 pe cy7
Manufactured by BD
Sourced in Switzerland
The Rat anti-CD4-PE-Cy7 is a flow cytometry reagent used for the detection and quantification of CD4+ T cells. It consists of a monoclonal antibody specific for the CD4 surface antigen, conjugated to the fluorescent dyes PE-Cy7. This reagent can be used to identify and characterize CD4+ T lymphocytes in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Live dead fixable violet dead cell stain kit, by Thermo Fisher Scientific (2 mentions)
Anti cd19 pe cy7, by BioLegend (2 mentions)
Rat anti mouse igg1 apc, by BD (2 mentions)
Rat anti cd8 pe cy5, by BD (2 mentions)
Hamster anti mouse anti cd3 fitc, by BD (2 mentions)
Rat anti mouse anti cd43 fitc, by BD (2 mentions)
Rat anti mouse anti cd45r b220 apc, by BD (2 mentions)
Rat anti mouse anti igm pe cy7, by BD (2 mentions)
2 protocols using rat anti cd4 pe cy7
1
Flow Cytometric Immunophenotyping of Mouse Immune Cells
2
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used for flow cytometric analysis: rat anti-CD4-PE-Cy7 (BD Pharmingen TM , Allschwil, Switzerland, #552775; 1:100); rat anti-CD8-PE-Cy5 (BD Pharmingen TM , Allschwil, Switzerland, #553034; 1:100); anti-CD19-PE-Cy7 (Biolegend, San Diego, CA, USA, #115520; 1:100); hamster anti-mouse anti-CD3-FITC (BD Pharmingen TM , Allschwil, Switzerland, #561827; 1:100); rat anti-mouse anti-CD43-FITC (BD Pharmingen TM , Allschwil, Switzerland, #561856; 1:100); rat antimouse anti-CD45R/B220-APC (BD Pharmingen TM , Allschwil, Switzerland; #553092; 1:100); rat antimouse anti-IgM-PE-Cy7 (BD Pharmingen TM , Allschwil, Switzerland, #552867; 1:100); rat anti-mouse IgG1-APC (BD Pharmingen TM , Allschwil, Switzerland; #550874; 1:100). A LIVE/DEAD™ fixable violet dead cell stain kit (ThermoFisher Scientific, Waltham, MA, USA; #L34955; 1:1000) was used to identify dead cells.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!